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Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果:提取液主要为Ⅰ型胶原蛋白;72小时37℃恒温培养箱内培养无细菌生长;紫外分光光度计测定,吸收高峰为230nm;氨基酸成分主要为甘氨酸:占40%,脯氨酸:14.3%。显微镜下见:相互交织的胶原原纤维结构组成的网状结构;纤维均匀,有规则横纹。胶原蛋白液在37℃恒温下,可制成凝胶,用于培养细胞;用磷酸液冲洗胶原蛋白凝胶可成固体片状,制成胶原膜,用作医用材料。

Methods Patients with acne were checked during clinical conventional examination,for the presence of tollicle flocculence and chaff spore bacteria,by examining with microscope the sebum or follicular keratotic plug from the skin lesion after10%KOH was added.

在门诊痤疮患者中常规检查毛囊虫和糠秕孢子菌,于皮损上挤出少量皮脂或毛囊角栓,置于玻片上,加10%KOH,盖上盖玻片,在显微镜下检查。

My eyes, bulging from staring too long into a florescent microscope, illustrate how easy it is to forget the human aspect of research.

我目不转睛地凝视着显微镜,很容易就忘却了研究中"人"方面存在的因素。

Methods The double flourescent staining of apoptosis and Desmin was examined in heart tissue of Keshan disease by immunohistochemical and TUNEL,and the confocal microscope was used to observe the results.

应用原位末端标记法及免疫组化技术在亚急型和慢型克山病患者心肌石蜡包埋组织切片上进行细胞凋亡及Desmin双重荧光染色,并应用共聚焦显微镜观察结果。

Flower-like ZnO nanorods were prepared with the hydrothermal method and characterized by X-ray diffraction and scanning electron microscopy.

利用水热法合成花状的ZnO纳米棒,并对其进行X射线衍射(X-ray diffraction, XRD与扫描电子显微镜(scanning electron microscope, SEM 表征。

Objective: To evaluate the usage of fluorescent microscope in analyzing cellular apoptosis.

目的 :探讨荧光显微技术在检测细胞凋亡中的作用。

The effects of As2O3 on HeLa cells and AsPC - 1 cells survival and apoptosis were determined by MTT assay and light microscope, and cellular ROS was also measured by fluorometer.

结果:2μmol/L As2O3处理HeLa细胞48h后细胞的生长受到明显抑制,表现出细胞凋亡的特征,随着As2O3浓度的增加和作用时间的延长效果更加明显。

In order to further confirm its subcellular localization , pYES2.0-MxIRT1::GFP with a inducible promoter was constructed and then transformed into the yeast strain by LiAc method. We observed the cells under the fluoroscope and laser confocal microscope through a method of Fm4-64, red and green fluorescent co-localized in plasma membrane.

根据结构预测MxIRT1是一种膜蛋白,为进一步确认它的亚细胞定位,利用构建好的诱导型酵母表达载体pYES2.0-MxIRT1∷GFP,通过GFP与活细胞荧光膜染料FM4-64结合的方法在荧光显微镜和共聚焦激光显微镜下观察。

The field of view can be limited by inserting a stop in the focal plane of microscope.

可用一架显微镜,在其焦平面上安设光阑来控制现

The test was conducted in the following manner; MM-164 liver carcinoma (1 x 10 [6] cells) was injected to left rear footpad of mice and the footpad was cut after 48 hours. Then, normal feed was given to the control group, 20% Maitake powder was given to group and 1 mg/Kg of D-fraction was intraperitoneally given for 70 times to group with normal food. All three groups were bred for another 30 days, and the number of tumor focus metastasized in the liver was counted by microscope.

我们进行了以下实验:MM-164肝癌细胞(1*106)注射到小鼠的左后足,在48小时后,截掉小鼠的左后足,组A:正常饮食作为对照组,组B:每天灌喂20%的灰树花粉,组C:腹腔注射1mg/Kg.bw的灰树花D-组分,三十天后处死,用显微镜检测肝部转移位点,结果见表5。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。