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The morphological change was observed with transmission electron microscope, compared with the control cells, the irradiated cells have more cytoclasis and cytolysis, the pykonosis and apoptosis corpus were also observed.

电镜下胞浆中空泡形成,细胞溶解、坏死,细胞核固缩,出现典型的细胞凋亡改变,形成凋亡小体。

Methods: The Hela cells were cultured in vitro and treated with different concentrations(0.125~2.0 mg/ml) of matrine solution for 24~72 hours. MTT assay was performed to evaluate the cytotoxin effect of matrine on Hela cells. FCM was used to detect the apoptosis of Hela cells. Transmission Electron Microscope analyses were carried out to examine the cell morphology.

选用人宫颈癌Hela细胞进行体外培养,以0.125~2.0 mg/ml的苦参碱分别处理Hela细胞24~72 h后,MTT法检测细胞增殖,流式细胞仪检测细胞凋亡率,透射电镜进行形态学观察,RT-PCR半定量检测凋亡相关基因Bcl-2和Bax的mRNA表达水平。

The gauge block surface under test is imaged by the interference microscope. The interference image is acquired and transmitted to the computer by the digital CCD video camera. Digital image processing technology is used to extract the edge contour line and midline of the interference dark fringe.

待测量块表面经干涉显微镜所成的干涉图像由数字CCD 摄像头采集并传输到计算机中,利用数字图像处理技术,对干涉条纹的边缘和中线进行提取,计算出量块表面粗糙度评定参数。

The seeds of Datura innoxia were set up in retrievable satellites.After returning to earth,the ultrastructural changes were observed by means of electron microscope.

以药用植物洋金花的种子为材料,搭载返回式人造地球卫星,回到地面后用电镜观察其超微结构的变化。

Among pesticides insecticides exhibited best compatibility,then herbicides,the last was fungicides.The process of mycelium of SYAU-06 strain infection to dayflower leaf was observed under light and electronic microscope.

结果表明杀虫剂与SYAU-06菌株的相容性最好,杀菌剂与SYAU-06菌株完全不相容,除草剂与SYAU-06菌株的相容性介于杀虫剂和杀菌剂之间。3。

The prior cycles with higher axial stress and larger strain range greatly restrains ratchetting strain of subsequent cycling with lower ones. Creep behavior plays an important role in the ratchetting strain and its influence on ratchetting behavior can not be neglected. Fracture morphology characteristics of two kinds of materials were investigated by scanning electron microscope. It shows that failure of material A is induced by cavitation and that of material B is induced by decohesion. For material A, with the decreasing strain amplitude the fracture surface shows more larger diameter globular particles under uniaxial loading and can be seen more obvious and deep crack under multiaxial loading. However for material B, with the decreasing strain amplitude fracture surface shows larger and larger scalelike shape and becomes more smooth under uniaxial loading, but fracture surface under multiaxial loading looks like more smooth than that of uniaxial loading, and shows gradually wave-like morphology.

通过扫描电子显微镜研究表明:材料A为空穴引起的失效,材料B为剥离导致疲劳破坏;随着载荷幅值的减小,材料A在单轴载荷下,断口表面浮出更多更大直径的球状颗粒,而多轴载荷下,可以看到很深的裂纹;随着载荷幅值的减小,材料B在单轴载荷下,断口表面鱼鳞片越来越大,表面越来越平整,而多轴载荷与单轴载荷相比,断口表面看上去更加平整,并且随着载荷幅值的减小,断口逐渐呈现波浪形貌;通过微观的傅立叶变换红外光谱分析,结果表明随着应变幅值的增加,无论载荷是单轴还是多轴状态下,橡胶材料吸光度比值随着幅值的增加而增加,这说明体系中的结晶含量也随之增加。

Methods Twenty guinea pigs with gentamicin-induced hearing loss were randomized equally into IGF-1 group and control group. In both groups, scala tympani fenestration was performed for injection of IGF-l (10μ1) or artificial perilymphatic fluid (10μl). Auditory brainstem responses test was performed before and 7 and 14 days after surgery, respectively, and the cochlea was removed by decollation of 3 guinea pigs from each group after ABR test for observing the changes in the hair cells using scanning electron microscope.

豚鼠20只,施用庆大霉素致耳聋后均分为IGF-1组和对照组,行鼓阶开窗术,微孔技术注入试剂(IGF-1组注入鼠重组IGF-1,对照组注入人工生理外淋巴液)10μ1,分别在术前和术后7、14d行ABR测试;测试完后每组3只断头取听泡,在扫描电镜下观察耳蜗毛细胞改变。

Histopathological changes of lung tissue were observed under light microscope. The protein expression of MMP (MMP-2, MMP-9) and TIMP (TIMP-1) in lung tissue were determined by immunocytochemistry. RESULTS: Decompensate respiratory acidosis (pH =7.20±0.04, PaCO2 =7.84±0.15) developed in group B.

用ELISA法测定大鼠血清MMP-2、MMP-9、TIMP-1活性及NE活性;弹力纤维染色标本进行弹性纤维相对含量的测定;免疫组化方法检测肺组织中MMP-2、MMP-9及TIMP-1的表达及光镜下进行病理学观察。

Electron microscope: endocytoplasmic reticulums expanding and breaking, ribosomes defluxion, lysosomes increasing, vascular endothelial cells swelling and blood vessels congestion appeared in all groups, and the mitochondria swelling is serious in the groups which pressures were more than 400 kPa.

电镜下可见内质网扩张、断裂,核糖体脱落,溶酶体增多,血管内皮细胞肿胀、血管淤血等在各压力均可发生,线粒体肿胀变形以400KPa以上压力组为明显。

Results By using optical microscope, the neurocytes of hippocampus in experimental group were observed to denaturalize, tumefy, destroy and disappear.

点燃癫痫鼠海马区损伤越重,胶质细胞GFAP免疫反应性越强,通过检测GFAP可以推测星形胶质细胞与癫痫后脑损伤的程度。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。