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Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

The function of oculomotor nerve was observed periodically, and neuroanatomical and histological aspects were observed with light microscope and electron microscope.

术后观察动眼神经功能并进行神经组织学和解剖学研究。

Wear model and characteristics of the second cutting deformation zone,that is,between the rake face of cutter and chips were investigated by carrying out diamond PCD cutter ultraprecision machining test of SiC noncontinuous reinforced aluminum composites,and measuring analysis with atom force microscope and scan electronic microscope.

通过金刚石PCD刀具对非连续S iC增强铝基复合材料的超精密车削加工试验，考察了刀具第二切削变形区的摩擦磨损性能，并提出了相应的模型；采用爆炸式快速落刀装置制备出切屑根并分析了积屑瘤的影响因素；采用原子力显微镜对PCD刀具的刃口磨损形貌进行观察，并分析其磨损机理。

Based on the development of key techniques,such as fabrication of optical fiber probe and probe-sample distance nonoptical control, ete,we have built a scanning shear force near-field optical microscope ,which can be combined with an inverted optical microscope.

在发展光纤探针制备和探针与样品近场间距非光学控制等关键技术基础上，我们研制成能与倒置光学显微镜联合使用的扫描切变力/近场光学显微镜，并具有反射和透射等工作模式以及能在溶液环境中工作。

Methods After ZTO injection acted on human ovarian cancer SKOV3 cells, the changes in the nucleus were observed by fluorescence microscope, oncosis index was counted by projection electron microscope, the situation of cell DNA breakage was observed by agarose gel electrophoresis, and cell Bcl-2 gene expression was observed by immunohistochemical method.

以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后，通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。

The authors applied a series of eperimental and clinical determine methods forths first time in our country, such as electron microscope, specular microscope and ulrra- sonic pachymeter, to analyse and compare the effect to the cormeal endothelium between the posterion chamber phaco and intereaprular phaco. Therefore, the author evaluated objec- tively the intercapsulat phaco and it developing prospect.

我们在国内首次运用电镜、镜面反射角膜内皮显微镜及超声角膜测厚等一系列实验室和临床检测手段，分析比较后房超声乳化和囊间超声乳化对角膜内皮细胞的影响，对囊间法超声乳化及其发展前景做出较客观地评价。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

The study performed by the authors is of significance in finding favorable reservoir zones in Dongying depression. Many thin sections were analyzed by such means as standard petrographic microscope, X-ray diffraction and scanning electron microscope analysis so as to study the selected samples.

在对大量薄片和岩心进行分析的基础上，运用扫描电镜和X衍射粘土分析等手段，对东营凹陷砂岩储层的成岩作用和孔隙演化进行了研究。

After being treated with melatonin for 8 weeks, pathological changes under photics microscope and electron microscope were observed.

3不同剂量Mel干预8周后，观察大鼠心肌组织在光镜和电镜下的组织学及超微结构的改变。

Cultured for 48 hours, the floating cells were removed and the adherent cells were continued to culture with cytokines including IL-4 and GM-CSF. After 14 days culture, the induced DCs were observed by photics microscope and electron microscope, also co-cultured with native T cells derived from spleen, its stimulating function was detected by MTT assay.

经过光镜，透射电镜观察培养DCs的形态学特征；通过同种T细胞混合培养，采用MTT比色分析法测定不同浓度的DCs发同种T细胞增殖的能力。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。