查询词典 microscope stage

Results: Ameloblastin mRNA was negative under microscope in the ameloblast proliferate differentiation period, it showed weakly positive expression in intra-cellular and new-birth enamel matrix in secrete initial stage while it showed strongly positive expression in intra-cellular and new-birth enamel matrix when it came to secretion phase.

结果：釉鞘蛋白mRNA在成釉细胞增殖分化期表达为阴性，分泌初期细胞内及新生釉基质中开始出现弱阳性表达；至分泌期，细胞内及新生釉基质中均呈强阳性表达。

Cell of Ela of 鳫 of fleeing Yu Piao, the stage longs for blue refus to catch a law to detect wither of cell of revulsive Hela of EVn-50 of observation of microscope of fluorescence of coloring of cellular vigor;AO/EB dies electrophoresis of gel of morphology change;DNA is corroborant wither of cell of EVn-50 revulsive Hela dies action;PI coloring sheds type cell appearance to detect wither of cell of EVn-50 revulsive Hela dies rate.

体外培养Hela细胞，台盼蓝拒染法检测细胞活力；AO/EB染色荧光显微镜观察EVn-50诱导Hela细胞凋亡形态学改变；DNA凝胶电泳确证EVn-50诱导Hela细胞凋亡功能；PI染色流式细胞仪检测EVn-50诱导Hela细胞凋亡率。

Results there were unconsciousness, respiratory rhythm disturbance and hyporeflexia of pupil light reflex immediately after injury, and reactiveness decrease and activity retardation still existed even after resuming consciousness. at anatomical scene, subarachnoid hemorrhage or cerebroventricular haemorrhage were widespread. at an early stage, there were swelling, collapse, and axonal retraction ball formation at cortico-medulla junction, callosum, brainstem, and cerebellar white matter under microscope. but at the later stage, gitter cell proliferation and nest-like aggregation were major pathophysiological changes at focal brain tissue.

结果 伤后大鼠均即刻出现原发昏迷，其中2只于损伤后20 min内死亡，余持续时间1-30 min不等；伤后大鼠呼吸节律紊乱，瞳孔对光反射减弱或消失，醒后均有程度不等的反应性下降，肢体活动迟缓；肉眼可见广泛蛛网膜下腔出血或脑室出血；光镜下可见皮髓交界区、胼胝体区、脑干、小脑白质等部位的神经轴索有不同程度的肿胀、断裂、轴索球形成，后期有小胶质细胞增生，局部呈巢样聚集。

Dimensions and positions of holes for stage clips and attachable mechanical stage on microscope stage

显微镜。载物台装压簧和移动尺用孔的尺寸和位置

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

The inhibition on the proliferation depended on the exposure time and dose.The cells showed morphological changes at the early stage of apoptosis under the light microscope:the shrunk and round cells;condensed cytoplasma and pycnosis nucleus and autophasosomes could be found under the transmission electron microscope.

在凋亡早期细胞形态学变化在光镜下可见细胞缩小变圆，在电镜上可见细胞质浓缩、核固缩及自噬溶酶体。4.0μmol/L As2O3作用72h后HT-29细胞凋亡率为25%。

Formation of infection thread and nodules at early symbiotic stage between 2-33 and A. sinicus were monitored in situ by using laser confocal scanning microscope and fluorescent microscope.

利用激光共焦扫描显微镜和荧光显微镜原位监测了标记菌株2-33与紫云英早期共生结瘤阶段的侵入线与根瘤形成过程。

Marlstone samples from the Early Cretaceous Shangkuh Formation in the Jianzishan section and geological pits in Dawu＇ergen were analyzed systematically using the polarizing microscope, fluorescence microscope and freezing-heating stage.

利用偏光显微镜、荧光显微镜和冷热台对大乌尔根尖子山剖面和地质浅井早白垩世上库力组的泥灰岩样品进行了系统研究。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

The user can make single polarizing view, orthogonal polarizing view, cone light view and micro-photograph. The microscope is equipped with attachment, such as gypsum,mica(1/4 ) test piece, quartz wedge and attachable mechanical stage. It is a novel microscope with maturity function and nicer quality

透射偏光显微镜就是非常适用的产品，可供广大用户做单偏光观察，正交偏光观察，锥光观察以及显微摄影，配置有石膏λ、云母 1/4 试片、石英楔子和移动尺等附件，是一组具有较完备功能和良好品质的新型产品。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。