查询词典 microscope

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

Results (1) CCD fluorescent microscope was superior to laser scanning confocal microscope in collecting the fluorescent image of DCF. CCD fluorescent microscope was chosen to be the system applied in this research with the consideration of operation, cost and et al. The mercury-arc lamp of fluorescent microscope was fit for the requirement of our study. The wavelength range of the light output was 460-490 nm and the power density was about 100mW/cm in the condition of our study. Organelle-cell fluorescence intensity ratio analysis was more suitable than other methods.

结果：(1)CCD荧光显微镜采集到的DCF荧光图像的质量优于共聚焦显微镜，同时综合考虑包括采图质量、光照射剂量的可控性、实验成本和操作简便性等诸多因素，最终确定了CCD荧光显微镜作为实验研究的工具；荧光军医进修学院硕士学位论文中文摘要显微镜的汞灯输出功率稳定，光斑均匀，经本实验所选取的光路系统后输出波长范围为460一490nm，功率密度约为1 00 mw/cm；细胞器一细胞荧光强度比值法对于荧光分布位置的确定优于其他方法。

Quality equipment categories: Programmable constant temperature and humidity box, high and low temperature test chamber, salt spray test machine, oven, heat aging test machine, cold / anti-yellowing chamber, hot and cold impact test machine, hot wire testing machine, Vertical + level of combustion testing machine, testing machine tracking, key life testing machine, plug and socket load life testing machine, spring testing machine, 90-degree peel strength test machine, the breakdown strength test machine, Central compressive strength testing machine, paper tube / carton compression testing machine, wearable rub tester, plug test machines, rotor life durability testing machine, testing machine Life expectancy clamshell mobile phone, mouse, roller-life testing machine, vibration test machine to simulate the vibration motor transport units, cell phone drop test machine, packaging drop test machine, torsion testing machine wire Qu, sudden pull plug wire tester, static tensile testing machine, wire bending rock testing machine, wire elongation testing machine, universal testing machine, tensile testing machine, drop-ball impact testing machine, fatigue life testing machine headphones, headsets Life expectancy tensile testing machine, push pull, the cloth, carvedilol, Victoria, Xiao, Lane, Shaw Sclerometer, video measuring instrument, precision mapping microscope, digital projectors, stereomicroscope , metallographic microscope, reading microscope, continuous zoom microscope, magnifying glass, caliper, micrometer, standard weight, height, the thickness, the slab of rock, electronic weight, said that electronic counting, electronic scales, analytical balance, standard light boxes, etc..

可程式恒温恒湿箱、高低温试验箱、盐水喷雾试验机、干燥箱、热老化试验机、耐寒/耐黄变试验箱、冷热冲击试验机、灼热丝试验机、垂直+水平燃烧试验机、漏电起痕试验机、按键寿命试验机、插头插座负载寿命试验机、弹簧试验机、90度剥离强度试验机、破裂强度试验机、环压强度试验机、纸管/纸箱抗压试验机、耐磨擦试验机、插拔力试验机、转轴寿命耐久试验机、手机翻盖寿命试验机、鼠标滚轴寿命试验机、振动试验机、模拟汽车运输振动台、手机跌落试验机、包装跌落试验机、电线曲挠试验机、插头线突拉试验机、静态拉力试验机、线材弯折摇摆试验机、线材伸长率试验机、万能材料试验机、拉力试验机、落球冲击试验机、耳机疲劳寿命试验机、耳机拉伸寿命试验机、推拉力计、布、洛、维、肖、里、邵氏硬度计、影像测量仪、精密测绘显微镜、数字投影仪、体视显微镜、金相显微镜、读数显微镜、连续变倍显微镜、放大镜、卡尺、千分尺、标准砝码、高度计、厚度计、岩石平板、电子计重称、电子计数称、电子天平、分析天平、标准光源箱等。

The main techniques in Nanobiology are scanning probe microscope and Optical Tweezer.

纳米生物学研究的技术手段目前主要是扫描探针显微术(Scanning Probe Microscope，SPM)和光钳技术。

In this thesis, rat-tails were used as experiment objects, there are 40 slices including transverse and longitudinal, HE-dyed and undyed. Excited by femtosecond laser, the changes of backward SHG and TPEF versus sample preparation, excitation wavelength, excitation power, penetration depth and so on were observed by two-photon laser scanning confocal microscope, effect factors of backward SHG and TPEF and differences of both were discussed and compared, and related comments were also presented.

以鼠尾组织作为实验对象，共40个切片，分为横向和纵向、HE染色和未染色四组，采用飞秒激光器作为激发光，用双光于激光扫描共焦显微镜(two-photon laser scanning confocal microscope， TPLSCM)观察和分析了样品在不同的制备方式、激发波长、激发功率、扫描深度等条件下的背向SHG和TPEF的变化曲线，讨论和比较了生物组织的背向SHG和TPEF的影响因素以及二者之间的异同，并尝试对实验现象做出了一定的解释。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

New technical terms have come into use, formed on latin or greek source-words because these can convey precise ideas in easily combinable forms, for example bacteriology, microscope, radioactive, and semiconductor.

新的技术术语在希腊语或拉丁语源词汇的基础上形成，并开始为人所用，因为这些词汇能以简便的组合形式准确地表达各种概念，如bacteriology、microscope、radioactive以及semiconductor等。

The full developmental stages from preovulatory oocyte to expanded blastocyst of mouse embryos were observed by inverted DIC microscope, and photos were taken by digital microphotography.

在这些研究中，使用微分干涉差显微镜(differential interference contrast microscope，DIC)技术，对小鼠的早期胚进行细致的观察与操作是不可或缺的关键步骤［1］。

The full developmental stages from preovulatory oocyte to expanded blastocyst of mouse embryos were observed by inverted DIC microscope, and photos were taken by digital microphotography.

在这些研究中，使用微分干涉差显微镜(differential interference contrast microscope，DIC)技术，对小鼠的早期胚进行细致的观察和操作是不可或缺的关键步骤［1］。

Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%, 86.8%, 90.5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84.3%, 88.1% and 83.3%, 87.9% respectively.

结果 观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%，与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%，与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%，流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。