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The tests have Simulated the rainstorm state and the order state, and the results indicated the disposal of microorganisms-aquatic plant system is efficient. The optimization removal rate time was ascertained under the static state test, in dynamic test, comparing and analyzing the effect on removal rates of different concentration wastewater. The results showed, without aeration, the removal rate of CODCr, TN was 85.9%,75.1%, respectively, the highest removal rate of TP was 64.6%, and the concentration of DO increased steadily from 2mg/L to 7mg/L during experiment. In dynamic test, the microorganisms-aquatic plant systems have also appeared better effect on freshet, and the removal rate of TN,TP and CODCr, was 64%,69% and 81%, respectively.

利用接种高效微生物菌剂促进填料快速挂膜,并和水生植物联合作用去除水体中的氮磷等污染物,在静态水体下研究达到最佳去除效率的时间,并在连续流试验下对比分析不同浓度下的去除效率,根据静态模拟试验结果表明,在不曝气,自然复氧条件下,CODCr和TN的最终去除率分别为84.3%和71.4%,TP最高去除率为62.9%,DO也从2mg/L稳定的升高到7mg/L左右;在连续流模拟试验结果表明,该系统对雨后入湖的河水中TN、TP及CODcr的去除率分别稳定在近64%,69%和81%以上。

Effect of SMF on Microorganisms (1) The influences of magnetic induction in SMF on microorganisms were studied.

以细菌、酵母和霉菌为对象,研究液体中不同强度静态磁场对微生物的影响,获得磁场作用24h后微生物的存活率与磁场磁感强度的拟合方程如下:(1)啤酒酵母〓(2)黑曲霉〓(3)枯草杆菌〓 2。

Soil-microorganisms system in two sites along Mosel river was chosen as typical soil-microorganisms system because the soil was highly polluted by PAHs and microorganisms were the majority of organisms.

土壤—微生物系统选择受PAHs污染严重且微生物为优势生物的Mosel河流沿岸土壤—微生物系统为例,研究结果发现,Temmels土壤添加的D_(10)-PA被很快释放,并被本土微生物迅速降解,在2周时间内降解了约92%,并在4周内降解了约99%,以后基本保持稳定。

To prevent epidemical disease prevalence caused by microorganisms in indoor air, a study was carried out to investigate the effect of ventilation, peracetic acid and ozonization on microorganisms reduction in the indoor air within a campus. The experimental results indicated that ventilation was an effective measure in reducing indoor air microorganisms with economy and simple operation.

为了防止室内空气微生物导致的传染病发生,对某校园室内的空气进行通风、过氧乙酸和臭氧灭菌降低微生物的实验研究结果表明,通风可以有效地降低室内微生物的浓度,是最为经济和易行的措施;过氧乙酸灭菌效果好,持续时间较长,可在不易实施通风情况下采用过氧乙酸灭菌;臭氧具有迅速杀菌的效果,使用时要与人保持一定距离,最好超出 1m。

The changes of nutrition in milk treated with different technologies are also determined. Results showed that, 1 higher pressure, longer holding time and more alternative times favour a larger destruction of microorganisms; 2 the combined effect of hurdle technology and pressure on the destruction of microorganisms in fresh raw milk is more effective than only using pressure treatment, among which the effect of ε-polylysine on inactivation of microorganism in fresh raw milk is more effective than that of Nisin with the same concentration of 100mg/L; 3 the whole proteins in milk treated by the different types of sterilization have been detected by SDS-PAGE electrophoresis, and comparing with the whole proteins' electrophoretogram of fresh raw milk, we found that the first spectrum band near the negative pole was disappeared and the colours of the second spectrum band was tint in UHT, pasteurism and 500MPa treated milks, which was suggested that some proteins' subunits were dissociated, but the 400MPa treated milk showed the similar eletrophoretogram with the fresh raw milk; 4 the loss of threonine, VA and VC in high pressure treated milk was more less than those in pasteurism treated milk, in particular threonine and VA were almost retained, and the contents of VB1 and VB2 in the former are also more than that in the latter; 5 high pressure treated milk had longer shelf life than pasteurism treated milk, extending the sale semidiameter of milk.

压力越高,保压时间越长,交变次数越多,杀菌效果越好;2)采用栅栏技术对牛乳中的微生物具有更有效的杀菌作用,其中相同浓度的(终浓度100mg/L)ε-聚赖氨酸较Nisin具有更强的杀菌效果;3)利用SDS-PAGE电泳研究了不同杀菌方式对牛乳总蛋白的影响,发现经UHT、巴氏和500MPa交变处理的牛乳与生鲜牛乳相比其总蛋白电泳图谱靠近负极方向第一条谱带消失,第二条谱带颜色变浅,由此推测经上述处理后牛乳中有一些蛋白质的亚基发生了解离,而经400MPa交变处理的牛乳与生鲜牛乳的总蛋白电泳图谱相似;4)超高压牛乳营养成分与生鲜牛乳相比,苏氨酸、VA、VC在超高压处理中受到的破坏明显比巴氏杀菌小得多,特别是苏氨酸和VA几乎完全被保留下来,而VC含量也要比巴氏奶高出25%以上,对于VB1、VB2,检测数据也表明,超高压灭菌对其破坏要比巴氏灭菌小,其含量分别比巴氏杀菌牛乳高出9.5%和2.6%;5)与巴氏杀菌乳相比,超高压杀菌有助于延长牛乳的保质期,扩大牛乳的销售半径。

PCR-SSCP (single-strand conformation polymorphism) was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed:(1) The pronucleus microorganism's community was similar and also had the polymorphism in each simple of various fermentation stage;(2) Different microflora had complex ecology effects of coordination and the restriction;(3) The diversity indexes of different stage of Daqu microorganisms were around 1.69~2.01, and the composition of them was stable relatively;(4) The similarity indexes were 0.67~1.00, and much higher in the approaching stages.

采用PCR-SSCP技术对浓香型大曲发酵过程中原核微生物群落结构的变化情况进行了研究,结果表明:(1)发酵各时期曲样的群落结构相似,同时也具有多态性;(2)不同微生物群落具有协同和制约的复杂生态学效应;(3)各时期曲样微生物多样性指数均在1.69~2.01之间,群落结构相对较稳定;(4)各曲样微生物群落相似性位于0.67~1.00之间,邻近时期样品间的相似性程度较高。

In order to understand the adaptation of the microorganisms under the extreme cold environment conditions, the physiological characteristics of the 5 bacterial isolates from the Muztag Am ice core under the different temperatures were investigated. The csp gene was also amplified by PCR technique and the specific primer targeted for the cold shock protein. The observation results showed that there were three phenotypes of microorganisms: psychrophiles growing from 0~23°С(the optimum temperature at 15°C), psychrotolerant with growth range at 0~40°C (the optimum temperature at 23°С), and the thermophile with growth range at 0~45°C(the optimum temperature at 30°C).

为了解冰川微生物的生理生长特征及其在极端寒冷环境条件下的冷适应特性,从慕士塔格冰芯分离菌株中选取了5个代表菌株,分别对这些菌株的生长温度和酸度范围进行了观测,并利用微生物冷诱导蛋白的保守氨基酸序列,设计了1对冷诱导基因csp的特异引物,并对其进行了PCR扩增。

Culturable microorganisms provide very limited information on soil microbial diversity,because only 0.1%-10% of soil microorganisms can be cultured under conventional laboratory conditions.

传统的基于培养的研究方法只能反映土壤中少数(0.1%~10%)微生物的信息,而大部分微生物目前还不能培养,因而这部分微生物资源尚难以被有效地开发利用。

Fermentative microorganisms and mixed functional microorganisms are two important microorganisms,which can be used in the production of bioorganic fertilizer extensively.

发酵微生物菌群和复合功能微生物菌群是两个重要的微生物功能菌群,在制备生物有机肥中有着广泛的用途。

Form、structure、characterization and function of prokaryotic、eukaryotic and molecular organisms;②nutrition and cultivation techniques of microorganisms;③physiological and metabolizable production of microorganisms;③theory of genetic variation and microbic breeding technique of microorganisms;⑤ecology and environmental protection of microorganisms;⑥Infection、 Immunity and biologics of microorganisms;⑦classification and identification of microorganisms and the international Code of Nomenclature of microorganisms.and microbial high-techs developing on these as the fundament of life sciences.

主要讲授:①原核微生物、真核微生物及分子生物的形态、结构、特点和功能;②微生物的营养和培养技术;③微生物生理及代谢产物;④微生物遗传变异理论和育种技术;⑤微生物生态和环境保护;⑥微生物传染、免疫和生物制品;⑦微生物分类鉴定和国际命名法则,以及在此基础理论上发展起来的微生物高新技术,是当代生命科学研究的基础。

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