查询词典 microinjection

Conditional rBDNF transgenic vector was successfully constructed and strong overexpression of rBDNF and EGFP were identified in the cotransfected cell line, and establishment of this targeting vector is critical for pronuclear microinjection.

rBDNF条件性基因载体构建成功，在体外细胞系中，检测到rBDNF及EGFP强表达，为转基因显微注射打下基础。

HEPO ; Milk ; goat ; Mammary gland ; expression ; Pronuclear microinjection

hEPO ；奶山羊；乳腺表达；原核注射

Methods: Microinjection technique was used to structure the human SERT heterologious system.

显微注射方法将 hSERT 基因 cRNA 注射到非洲爪蟾卵母细胞内，建立异体表达系统。

In order to observe the influences of the descending projections from the subnucleus reticularis dorsalis on the neurons in the spinal cord and caudal spinal trigeminal nucleus,by using an immunocytochemical method,we investigated the c-fos expression within spinal cord and Vc after kainic acid microinjection into the SRD of the rat.

为了观察起自延髓网状背侧亚核的下行投射对脊髓和三叉神经脊束核尾侧亚核神经元活动的影响，将微量海人藻酸注入大鼠SRD中，致SRD神经元兴奋，在SRD下行通路的作用部位引起c-fos的表达。

Microinjection of norepinephrine (NE, 30 nmol) into the subnucleus commissuralis of nucleus tractus solitarii of rabbits evoked a decrease of mean arterial pressure and heart rate. These effects were attenuated by application of naloxone (3 nmol) on the same area,which by itself showed no effect on MAP and HR.

于杂种白兔的孤束核联合亚核内微量注射去甲肾上腺素，引起血压降低和心率减慢，将小剂量阿片受体阻断剂纳洛酮注入CNTS，本身不影响血压与心率，但减弱NE在CNTS中的降压降率幅度。

In this study, based on establishment of stable and high efficient transfergenic receptor system with supersweet maize callus, the multiple insect-resistant genes were successfully co-transformed into a elite inbred line 1132 and a variety using particle bombardment and overy microinjection, and the transgenic integration, expression and inheritance were studied by means of target character and molecular biology method.

本研究在建立高效稳定基因转化受体系统的基础上，利用基因枪和子房注射两种方法将多个抗虫基因成功转化抗性较差的超甜玉米自交系及其品种，并从分子水平和目标性状上研究外源基因的转入、整合、表达与遗传规律。

On the basis of the above studies, the effects and mechanisms of microinjection of glucocorticoid or mineralocorticoid into the rVLM on the arterial blood pressure, HR, pressor and tachycardiac responses produced by stimulation of the dorsal periaquaductal gray in the midbrain were further observed and analysed in the acute experiments on normotensive rats.

在此基础上，进一步在急性动物实验中观察分析肾上腺糖、盐皮质激素在rVLM区对血压、心率、电刺激防御反应区诱发的防御性升压和加快心率反应的作用及其机制。

Microinjection of glucocorticoid or mineralocorticoid (40 or 10 ng/0.1 ul/site) into the rVLM bilaterally caused an increase in BP, HR and the pressor response induced by the dPAG stimulation in the normotensive rat, respectively. Dose-dependence of both drugs was observed on the above effects in three directions. Nevertheless, there was no effect of both drugs on the tachycardiac response induced by the dPAG stimulation.

二、分别微量注射糖、盐皮质激素(40或10ng/0.1μl/site)至双侧rVLM，可引起正常血压大鼠的BP升高，HR加快，以及电刺激中脑中央周围灰质背侧区诱发的防御性升压反应增强，并均呈现出剂量-效应依赖性关系，而对电刺激dPAG诱发的加快心率反应无明显改变作用。

After microinjection of the antagonists of glucocorticoid RU 38486 or mineralocorticoid spironolactone (40 ng/0.1 ul/site, respectively) into the rVLM bilaterally, a decrease in BP, HR and the pressor response induced by the dPAG stimulation was observed in the normotensive rat, and there was no change on the tachycardiac response induced by the dPAG stimulation.

分别微量注射糖、盐皮质激素的拮抗剂RU 38486(40ng/0.1μl/site)或螺旋内酯(40ng/0.1μl/site)至双侧rVLM，则引起正常血压大鼠的BP下降、HR减慢，以及电刺激dPAG诱发的防御性升压反应减弱，对电刺激dPAG诱发的加快心率反应无明显改变作用。

Methods: AD rat model was established by microinjection of amyloid-beta protein (Aβ1-40) into the Meynert nucleus of rats. We acupunctured "baihui (DU 20)""taixi (KI 3)""zusanli (ST 36)" to observe the effect of electrotherapy on AD rats, the memory ability was studied by Morris water maze, and ultrastructure of neurons was observed under transmission electron microscopy.

Meynert核注射微量Aβ1-40制备动物模型，选取百会、太溪、足三里电针治疗，以Morris水迷宫评价大鼠记忆能力，并运用电镜观察AD大鼠额叶病理超微结构变化。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。