查询词典 microinjection

The ventilatory responses of the peripheral chemoreflex following microinjection within the NA of either P2X receptor agonist adenosine triphosphate or antagonist pyridoxal-5′-phosphate-6-azo-phenyl-2, 4-disulfonate were observed.

结果 吸入低氧混合气后，动物呼吸加深加快；在NA处微量注射ATP增强了急性低氧呼吸频率，微量注射PPADS部分阻断了ATP对低氧通气的兴奋效应。

Nuclear transfer is a new cloning technology developed in recent years. NT methods consist of electrofusion,NT mediated by polyethylene glycol and microinjection.

核移植技术是近年发展起来的细胞克隆技术，核移植的方法有电融合法、PEG介导法和微注射法。

Microinjection of histamine into the third cerebral ventricle can produce excitory effect on respiratory activities of guinea pigs possibly via H1 receptors.

第三脑室微量注射组胺有兴奋呼吸的作用，该作用可能是由组胺H1受体所介导的。

Microinjection of glutamate into the vMNF caused inhibitory response of the myoelectric activities of genioglossus.

结果表明：vMNF的兴奋能降低颏舌肌的肌电活动，从而提示其具有增强上呼吸道阻力的作用。

The results are as follows.(1) Long train electrical stimulation at the dMNF facilitated myoelectric activities of genioglossus and phrenic muscle.(2) Short train electrical stimulation of dMNF, when delivered in the inspiratory phase, caused prolongation of myoelectric activities of genioglossus and phrenic muscle; but when delivered in the expiratory phase, myoelectric activities of genioglossus and phrenic muscle were shortened.(3) Long train electrical stimulation at the vMNF inhibited myoelectric activities of genioglossus and phrenic muscle.(4) Short train electrical stimulation of vMNF caused shortening of myoelectric activities of genioglossus and phrenic muscle, when delivered in the inspiratory phase, but a prolongation when delivered in the expiratory phase.(5) The effects of microinjection of glutamate to dMNF and vMNF were similar to those of electrical stimulation.

结果如下：(1)长串电脉冲刺激dMNF引起颏舌肌和膈肌肌电活动明显增强；(2)短串电脉冲刺激dMNF，当刺激落位于吸气相时，引起颏舌肌和膈肌在吸气相的肌电活动延长，当刺激落位于呼气相时，引起颏舌肌和膈肌在呼气相的肌电活动提前终止；(3)长串电脉冲刺激vMNF使颏舌肌和膈肌肌电活动明显被抑制；(4)短串电脉冲刺激vMNF，当刺激落位于吸气相时，引起颏舌肌和膈肌在吸气相的肌电活动提前终止，当刺激落位于呼气相时，引起颏舌肌和膈肌在呼气相的肌电活动延长；(5)在dMNF和vMNF分别微量注射谷氨酸钠，其效应与电刺激结果基本一致。

Results Microinjection of L-glutamate into lateral habenula decreased wake time ; while increased REM sleep time.

结果 用L-谷氨酸兴奋LHb时，觉醒减少，快眼动睡眠增加；而电损毁LHb时，觉醒增加，REM睡眠减少。

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中，经Southern blot法检测有4头羊含有hEPO基因，其中3头为母羊，1头公羊于出生后20d死亡；3头转基因母羊hEPO基因的拷贝数分别为1、10、2；Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa；MTT法检测结果表明，在泌乳10d的3只转基因羊乳汁中，每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

However, pronuclear microinjection has been proven to be inefficient in producing transgenics and in inserting a piece of DNA into a specific site in the host genome.

但该方法存在整合效率太低及不能定点整合的问题。

Furthermore, with only a half -dose of DNA in the current system, by the horizontal system under×100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外，利用该系统还可在低倍镜下×100）将DNA常规注射量减半注射，提高了原核胚注射后的成活率，其注射胚的外源基因整合率亦明显提高。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。