查询词典 microinjection

The production efficiency of tansgenic animal by the traditional method of pronucleus microinjection is very low (The efficiency of transgene is about 3～5%).

以传统的原核显微注射法生产转基因动物的效率非常低下（成功率仅为3~5%），严重地制约了转基因动物研究进展。

Theproduction efficieny of transgenic animal by the traditional method of pronucleus microinjection isvery low (The effciency of transgene is about 3～5％.), which seriously restricted the developmentof transgenic animal research.

以传统的原核显微注射法生产转基因动物的效率非常低下(成功率仅为3～5％)，严重地制约了转基因动物研究进展。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法，制备hbv转基因小鼠，用pcr，elisa，荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

Objective To evaluate the feasibility of establishing transgenic mice by means of seminiferous tubule microinjection and electroporation in viva.

目的 探讨在体睾丸生精小管注射合并体内电穿孔建立转基因小鼠的可行性。

3D control of micropositioning, the environment for human-computer interaction based on the micro stereo image displaying, and microinjection are three focuses of this dissertation.The imaging models of CMO micro stereoscope and G micro stereoscope used in microvision subsystem are deduced. According to the imaging models, 3D visual servoing model based on image for micropositioning is set up. The technology of tracking and matching on the feature of image, which are relevant in visual servoing, is studied. Two arithmetic for avoiding obstacles, rolling/surrounding and curved tracking based on the depth first search, are further discussed.

在此基础上，对三维微定位控制技术、基于显微立体图像显示的人机交互操纵环境和微量注射等三个内容进行了重点研究：为了解决3D微定位问题，推导了显微视觉分系统采用的CMO型和G型体视显微镜光学成像模型，在此基础上建立了基于图像的3D显微视觉伺服定位控制模型，对视觉伺服中涉及的实时跟踪与立体匹配技术进行了研究，并进一步探讨了卷包裹法和曲线跟踪两种避障算法。

There are significant differences between the two methods. Conclusion Clysising fimbriae tubae is a better embryo collecting method for rat microinjection.

冲洗输卵管伞端采集胚胎的方法为大鼠显微注射前胚胎采集比较好的方法。

The toxicity of cryoprotectans increased as the concentration of its increased.3, the survival rate of embryos microinjected with 6M PM was 25.07±1.57% after being coolled in -20℃ for 10 minutes by programmed cooling method, whenas ,the survival rate of controls dealt with five steps balance method was 20.88±2.84%, which indicated that the chilling sensitivity of embryos with microinjection decreased.

而任何一种抗冻剂均随着浓度的增加其毒性随之增加。3、另外，在冷敏感实验中，显微注射6M的PM的胚胎胚胎，应用程序化法处理，以2℃/min的速率降至-20℃，平衡10min后解冻处理，发现注射PM的胚胎低温处理后成活率为25.07±1.57%，而6M的PM五步平衡法同步处理的对照组胚胎成活率为20.88±2.84%，说明显微注射的胚胎冷敏感性一定降低。

After microinjection of the pEGFP-C1 vector in to fertilized zebra fish eggs, the expression rate was 80%.

在显微注射绿萤光蛋白进入斑马鱼授精卵后具有80%表现率。

Microinjection and particle gun were used to transfer pCMV-β into fertilized zebra fish eggs. After staining analysis, the data showed80% and 6% expression rates, respectively.

此研究利用显微注射与粒子枪基因转殖法转殖pCMV-β质体进入斑马鱼授精卵，经染色分析得到80%与6%之表现率。

The ventilatory responses of peripheral chemoreflex following 0.1 m l microinjection within the NTS of either PTK inhibitor genistein(10 mol/L), AMPA glutamate receptor inhibitor CNQX (10 mol/L),or inactive PTK inhibitor daidzein (10 mol/L) were recorded.

在孤束核处分别微量注射蛋白酪氨酸激酶的抑制剂genistein和其非活动性抑制剂daidzein 以及 AMPA受体阻断剂CNQX，观察这些药物对外周化学感受性反射的影响。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。