查询词典 microinjection

Objective To investigate the effects of microinjection of exogenous ghrelin into the hypothalamic paraventricular nucleus on gastric motility and electrical activity in ambulant conscious rats and their peripheral neural mechanisms.

目的 探讨下丘脑室旁核(paraventricular nucleus，PVN)内微量注射ghrelin对清醒大鼠胃运动及胃电活动的影响及其自主神经机制。

One of the cannulae was used for microinjection, and the other was used for microinjection or perfusion.

这些结果提示，脑内与吗啡镇痛有关的上述四个核团很可能通过神经纤维联成环状的回路。

On the normotensive rat, pretreatment with verapamil (0.25 ug/0.1 ul/site), the blocker of calcium channels, by microinjection into the rVLM bilaterally, not only caused a complete block effect on the elevation of BP, HR and the pressor response to defense stimulation by the subsequent microinjection of physostigmine, glucocorticoid or mineralocorticoid (the same dosage as mentioned above) into the same sites, but also led to a slight reduction on the basal cardiovascular activities to a certain extent.

四、在正常血压大鼠上预先用钙通道阻断剂维拉帕米(0.25μg/0.1μl/site)微量注射至双侧rVLM，不仅能阻断随后在同部位微量注射毒扁豆碱(0.4μg/0.1μl/site)、肾上腺糖、盐皮质激素(分别为40ng/0.1μl/site)所致的升压、加快心率、以及电刺激dPAG诱发的防御性升压反应，而且能引起上述指标不同程度的较弱的降低。

This experiment is for the purpose of unifying the porine ovocyte in vitro mature raise and the ROSI correlation operation, establishes in the ovicell nature the round sperm cell microinjection fertilization technology procedure, the exploration porine round sperm cell micro fertilization possibility, the research affects this kind of special cell microinjection fertilization effect the factor, for further studies the porine ROSI technology to provide the basic data.

本试验旨在结合猪卵母细胞体外成熟培养及ROSI相关操作，建立卵胞质内圆形精细胞显微注射受精技术程序，探索猪圆形精细胞显微受精的可能性，研究影响这种特殊细胞显微注射受精效果的因素，为进一步研究猪ROSI技术提供基础资料。

In this study, a new horizontal microinjection system has been established to solve these problems. Because the average time per pronuclear embryo by microinjection has been significantly shortened with the horizontal system, the cleavage rate and 16-cell rate of those microinjected embryos increased markedly.

为了解决这些问题，本研究有针对性地设计了新型的水平微注射系统，利用此系统，可明显缩短常规注射原核胚的平均时间，注射后原核胚的分裂率、8-16细胞胚胎发育率也明显提高。

On the normotensive rat, bilateral microinjection of L-arginine (16 n mol/0.1 ul/site), a precursor of nitric oxide into the rVLM, caused a decrease in BP, HR and the pressor response induced by the dPAG stimulation, while microinjection of NG-methyl-Larginine (L-NMMA, 16 n mol/0.1 ul/site), a blockade for NO to form, into the rVLM bilaterally, caused an increase in BP, HR and the pressor response induced by the dPAG stimulation. There was no effect of both drugs mentioned above on the tachycardiac response induced by the dPAG stimulation.

五、在正常血压大鼠，于双侧rVLM微量注射一氧化氮前体(16nmol/0.1μl/site)可引起BP降低、HR减慢、以及电刺激dPAG诱发的防御性升压反应减弱；而于同区微量注射生成一氧化氮的拮抗剂N〓-甲基-L-精氨酸(16nmol/0.1μl/site)则可引起BP升高、HR加快、以及电刺激dPAG诱发的防御性升压反应增强；而上述两药对电刺激dPAG诱发的防御性加快心率反应均无明显作用。

It is tentative investigated the transferring of mitochondria by microinjection. It is found that the key of the success of transferring of mitochondria by microinjection is not the microinjected from pollen grain trema, but the cultivated of the microinjected pollen grain.

对显微注射转移植物线粒体的技术进行了探索性研究，结果表明，该技术的难点不在通过花粉粒萌发孔进行mtDNA的显微注射，而在注射后花粉粒的培养。

Sixty-two artificially respired rabbits were anesthetized with urethae.Intranuclear microinjection was adopted.Microinjection of TRH into Lc-Sc induced a marked increase in respiratory frequency and significant elevation of blood pressure.The above mentioned effects of TRH into Lc-Sc on RF could be blocked by preinjection of α2 or β receptor antagonist into RVLM respectively.

实验选用62只行乌拉坦静脉麻醉和人工呼吸的家兔，采用脑内核团微量注射的方法，将TRH微量注入蓝斑复合核，使呼吸频率明显增快，平均动脉血压升高；在延髓头端腹外侧区分别预注射α2或β受体阻断剂，使TRH在蓝斑复合核的呼吸增频效应明显减弱，其升压效应可被延髓头端腹外侧区分别预注射α或β受体阻断剂拮抗。

Analysis of the relation between serotonin and its receptor in OFC and stress depression(n=8):(1) Microinjection of 5-HT into OFC significantly shortened immobility time, dejections have no great changes; though locomotor、 grooming activitives have little increased, they have no significant difference; rearing scores greatly reduced;(2)microinjection of pCPA into OFC significantly prolong immobility time, dejections have no great changes; locomotor、rearing activities are greatly reduced, but have no significant effect on grooming activity receding;(3)compared with the effect of 5-HT alone, microinjection of spiperone combined with 5-HT into OFC, significantly prolong immobility time and greatly reduced dejections; locomotor、 rearing、 grooming activities are all reduced, but have no significant differences.

眶额叶区5-羟色胺(5-hydroxytryptamine，5-HT)及其受体与应激性抑郁症的关系(n=8)：(1)注射5-HT后，大鼠FST不动时间明显降低(P＜0.05)，粪便数量变化不显著(p＞0.05)，OFT水平运动、修饰得分虽均有升高，但无显著性差异(P＞0.05)，垂直活动显著降低(P＜0.05)；(2)注射色氨酸羟化酶抑制剂-对氯苯丙氨酸后，FST不动时间明显增加(P＜0.01)，粪便数量无显著性变化(p＞0.05)，OFT水平、垂直运动显著减少(P＜0.05)，修饰降低但无显著差异(P＞0.05)；(3)与5-HT组比较，先注射5-HT_(1A)受体拮抗剂后再注射5-HT，FST中的不动时间明显增加(P＜0.01)，粪便数量显著减少(p＜0.01)，但OFT水平、垂直、修饰活动均有所减少，却无显著性差异(P＞0.05)。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。