查询词典 micrococcal

Generation of spheroplasts and chromatin hydrolysis with micrococcal nuclease.

代spheroplasts和染色质水解微球菌核酸。

So translational positioning determines which regions are more accessible (at least as judged by sensitivity to micrococcal nuclease).

在10bp的序列上移置DNA以更多的暴露连接区，但并不能改变DNA组蛋白表面保护一面以及被暴露在外的区域。

Positioning is examined by using micrococcal nuclease to examine cleavage sites relative to a restriction site at the 5 end of the gene.

最初基因会展现一个核小体的模型，它是由穿过基因一段有效距离的启动子构成的；在3ˊ端范围中定位作用遗失。

The spheroplast suspension (200 ul) was transferred into a microcentrifuge tube, supplemented with 150–350 units/ml micrococcal nuclease (Fer- mentas), and incubated at 37°e for 5 min.

暂停的原生质（ 200 UL ）的是转入微量管，补充与150-350单位/毫升微球菌核酸酶，并培养在37 ° E的5分钟。

Core particles ) Particles resulting from micrococcal nuclease digestion of nucleosomes, consisting of 140-bp DNA and the histone octamer of a nucleosome.

在真核生物中，双螺旋的 DNA 分子围绕一蛋白质八聚体进行盘绕，从而形成特殊的串珠状结构，称为核小体。

This method conformed the following conditions: spleen phosphodiesterase and micrococcal nulcease for digesting reagent and the concentration, temperature and digesting time of the two enzymes;the components of p32-post label alleviant and labeling time;the concentration of p1 nuclease; the concentration of T4 polynucleotide kinase for catalysing and labeling reagent;the components of bidirectional spreader and the spreading time ,etc.

确定了采用脾磷酸二酯酶和微球菌核酸酶消化底物以及两种酶的使用浓度、温度及消化时间；确定了P32标记缓冲液的主要成分及标记时间；确定了P1核酸酶的浓度；确定了T4多核苷酸激酶催化标记底物的浓度；确定了双向展开剂的主要成分及展开时间等。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

Micrococcal nuclease hypersensitive DNA fragments were either cloned directly, or mapped onto the genome by hybridization with oligo-nucleotide probes on a 244K Agilent array-CGH chip. The array-CGH mapping was confirmed for nuclease sensitivity by PCR after MNase digestion and by Southern blot analysis.

我们利用小球菌核酸酶超敏感的去氧核醣核酸片段直接选殖或是将其作为寡核苷酸探针於安捷伦244k寡核苷酸阵列-比较基因体杂合晶片上，进行基因体定位，并利用聚合酶链索反应和南方墨点法侦测其核酸酶敏感程度，以确认结果之可靠性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。