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To explain relationship between the effect of CLD on senile Demention and neuron in hippocampal area. The change of natural aging mice's neuron in hippocampal area were studied by applying microscope and transilluminating electric microscope. Results: Comparing with the young control, the old control mice's hippocampus had less small pyramid cells, the nerve cells denaturalizing and their branches reducing in the microscope. Hippocampal nerve cells were degenemiceing, with nucleus membrance crinkling like wave shape were not clear, and chromatin condensing with high electron density, heterochromatin accruing, part cell organs inclosing nucleus, nucleus condensing in the EM.

为了阐明菖龙丹抗老年性痴呆作用与海马区神经元结构的关系，运用光镜和透射电镜观察了菖龙丹对自然衰老小鼠海马区形态的影响，结果显示老年对照组小鼠光镜下海马锥体细胞较青年对照组数量减少，细胞体积变小，神经元变性，分枝减少；电镜下海马神经细胞发生退化改变，核膜皱缩，呈波浪状，膜结构不清晰，核染色质浓缩，电子密度高，异染色质较多，部分胞浆细胞器向细胞核聚集，有的可见细胞核固缩。

Methods Balb/c mice were immunized subcutaneously three times （0,2,4 weeks） with rSj 32 emulsified with adjuvant. Each mouse was infected with （40±1） curacies of Sj （6 weeks）. After 42 days of infection, the mice were killed and the worm number was counted.

用rSj 32×103加佐剂免疫小鼠3次，分别在0、2、4周进行，第6周每鼠经腹部感染（40±1）条日本血吸虫尾蚴，感染后42 d剖杀，计算减虫率。

LA795 cells were inoculated subcutaneously into the dorsa of T739 mice, and the mice were randomized into 3 groups.

将接种LA795肺腺癌细胞的T739小鼠随机分成3组，分别给予rhES，hAS或等体积PBS皮下注射，1次／日，共14d。

METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C，在证实其可以在真核细胞中表达之后，将其用脂质转染剂包裹，形成LipofectAMINE-pcDNAHCV-C脂质混合物，将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌，以空载体pcDNA3做对照，ELISA法检测血清中抗体产生水平。

Methods:Endometria of mice in the early phase of pregnancy and estrus cycle were collected and divided into 8 study groups,and every group included 10 mice.

收集动情周期各期和早孕2 d、3 d、4 d、5 d小鼠子宫内膜，共8组，每组10只小鼠。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

METHODS: By intraperitoneal injection of galactose, the hypergalactosemia mice models were induced. The lens of the mice were enucleated and were observed by light microscopy and electron microscopy in the early stage of the disease.

应用Wistar大鼠幼鼠采用腹腔注射半乳糖的方法诱发高半乳糖血症，在发病早期阶段取晶状体，分别应用光学显微镜和电子显微镜观察晶状体结构的改变。

Ten mice were exposed to 75% oxygen for 5 days,followed by 5 days room air recovery, the other ten mice of the same age were kept in room air as colltrol.All animals were killed on day 17 of life and both eyes were enucleated,and embedded in paraffin.

将高氧组10只幼鼠置于氧浓度约为75%的密闭环境中连续生活5d后回到正常环境下继续饲养5天，建立高氧诱导的视网膜新生血管的动物模型。

Objective: Design and synthesize the oligodeoxynucleotides containing unm- ethylated CpG dinucleotides to screen out the optimal CpG-ODN that could markedly enhance the immune function of cord blood mononuclear cells in health newborns. Moreover, observe the action of CpG-1826 (5'-TCCATGACGTTC CTGACGTT-3') as the immune adjuvant of the hepatitis B vaccine on the pregnant mice and their neonatal mice.

目的：设计并合成含非甲基化胞嘧啶和鸟嘌呤二核苷酸的寡聚脱氧核糖核苷酸，以期筛选出对新生儿脐血单个核细胞免疫刺激作用较强的CpG-ODN；观察不同剂量CPG-1826(5'-TCCATGACGTTCCTGACGTT-3')辅助乙肝疫苗免疫孕鼠及仔鼠的作用。

Methods The p21 HBsAg/HBsAg transgenic mice(2,6,12, 18, 24 months) in SPF grade and corresponding p21 +/+ mice were dissected and the liver and hepatic tumor tissues were extirpated for the examination by histological HE staining and electron microscopy.

方法分别选取2，6，12，18，24月龄的SPF级p21HBsAg/HBsAg转基因小鼠和p21+/+野生型小鼠，剖检进行大体观察，取肝脏及肝脏肿瘤组织，进行组织学HE染色及电镜超微结构观察。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。