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Results:UU positive rates from the lung and spleen in the newborn mice by UU infection(3.5×10~4 CCU/ml,5×10~5 CCU/ml,and 10~6 CCU/ml) were 0 and 0,2.7 and 1.4,and 74.4 and 30.3,respectively.Conclusions:The UU vertical transmission animal model during the course of cyesis was constructed successfaly through genital infection with UU in the pregnant mice.

目前关于妊娠期UU感染对围生期疾病的研究仅限于临床病例的调查分析,因此建立一个妊娠期UU感染垂直传播的动物模型,对于进一步研究妊娠期UU感染与不良妊娠结局之间相关性并阐明其发病机制十分必要。

The research aimed to study the effect of Cynanchum komanovii alkaloid on cellular immune function of mice 32 mice were divided into three experimental groups given medicament with daily dose of 12,24,48 mg/kg.bw and a control group infused with 0.5 ml/d distilled water in 30 days.

研究牛心朴子生物碱对小鼠细胞免疫功能的影响。取32只小鼠,按灌药剂量分为12、24、48mg/kg.bw3个试验组和1个蒸馏水对照组(0.5ml/d),连续给药30d,分别观察牛心朴子生物碱对小鼠体重、脏器指数、腹腔巨噬细胞吞噬作用及外周血T淋巴细胞数量的影响。

The mice were killed 3, 7 and 14 days after operation (8 mice per group at a time), the tissue of cystiform was harvested to receive gross, histology and immunohistochemistry observation, as well as RT-PCR and Western blot detection.

术后观察动物一般情况,于给药后3、7 及14 d,每组取8 只小鼠背部囊壁组织行大体、组织学观察及免疫组织化学、RT-PCR、Western blot 检测。

Objective To investigate the protective effect of ligustrazine on brain injury.Methods The effects of ligustrazine on breath time of head decollated mice and survival time of hypoxic mice induced by sodium nitrite were observed and neurological score and infarct area in the middle cerebral artery occlusion rats were measured.

目的 研究川芎嗪对脑损伤的保护作用方法采用小鼠断头法、亚硝酸钠(NaNO2)致死法、鼠大脑中动脉栓塞法,通过观察断头喘息时间、亚硝酸钠致死时间、脑缺血行评分、脑缺血面积,明确川芎嗪对脑损伤的保护作用。

Methods effects of ligustrazine on breath time of head decollated mice and survival time of hypoxic mice induced by sodium nitrite were observed and neurological score and infarct area in the middle cerebral artery occlusion rats were measured.

采用小鼠断头法、亚硝酸钠(NaNO2)致死法、大鼠大脑中动脉栓塞法,通过观察断头喘息时间、亚硝酸钠致死时间、脑缺血行为评分、脑缺血面积,明确川芎嗪对脑损伤的保护作用。

Methods The effects of ligustrazine on breath time of head decollated mice and survival time of hypoxic mice induced by sodium nitrite were observed and neurological score and infarct area in the middle cerebral artery occlusion rats were measured.

采用小鼠断头法、亚硝酸钠(NaNO2)致死法、大鼠大脑中动脉栓塞法,通过观察断头喘息时间、亚硝酸钠致死时间、脑缺血行为评分、脑缺血面积,明确川芎嗪对脑损伤的保护作用。

Results Ligustrazine (20mg/kg,50mg/kg) obviously prolonged breath time of decollated mice and survival time of hypoxic mice induced by sodium nitrite; ligustrazine (10mg/kg,20mg/kg) markedly ameliorated rat neurological deficit, reduced infarct area in MCAO rat.

结果 川芎嗪20mg/kg、50mg/kg可明显延长断头小鼠的喘息时间及亚硝酸钠致死小鼠的生存时间;10mg/kg、20mg/kg可显著改善中动脉栓塞致脑缺血大鼠的行为障碍,降低脑缺血梗死面积。

The time of cornea diabrosis, metastases into cervical lymph nodes and lung, survival time and ratio of CD4+ and CD8+ T cells in peripheral blood were observed.RESULTS: The time of cornea diabrosis in GTVC group or GTV group was later than that in PBS group, but no statistical difference between GTVC group and GTV group. Metastatic incidence in cervical lymph nodes of GTVC group mice or GTV group mice was lower than that in PBS group 18 days after the model had been established. There was no statistical difference in metastatic incidence between GTV group and GTVC group.

将荷瘤模型鼠分为磷酸盐缓冲液(phosphatebuffered saline,PBS)治疗组,IL12基因修饰肿瘤细胞疫苗(gene modified tumor cell vaccine, GTV)治疗组及GTV 联合CpGODN治疗组;鼠荷瘤模型建立后的第3d及第10d分别在各组鼠结膜下注射30μL PBS,30μL(3.0×106个细胞)灭活的GTV及含20μg CpGODN灭活的GTV,进行角膜溃破时间、淋巴结和肺部转移瘤发生率、生存时间及外周血CD4+和CD8+T细胞比例的观测。

This experiment adopted organic solvent extraction step by step,following deproteinization,dialyzation,alcohol-precipitation and layer-separation of dextran,to reveal the unknown effective ingredients in pumpkin,which have influence to blood sugar in normal mice or model mice of diabetes mellitus.

为探寻南瓜中对正常及糖尿病模型小鼠血糖有影响的未知有效成分,本实验依次采用有机溶剂分步萃取、除蛋白、透析、乙醇沉淀及葡聚糖柱层析等分离手段,以小白鼠血糖值作为筛选活性成分的指标,在分离的每一阶段对分离所得各个组分进行活性定量评估,并追踪活性最强的部分,最后分离纯化出降血糖活性最强的组分G———南瓜多糖。

U. S. EPA' s long carcinogenic test reported that dichloroacetic acid could induce hepatic cancer in rat and mice, and trichloroacetic acid could induce hepatic cancer in mice.

美国环境保护局长期致癌试验研究报告,二氯乙酸(dichloroacetic acid, DCA)可分别导致大鼠和小鼠肝癌,三氯乙酸(Trichloroacetic acid, TCA)可导致大鼠肝癌。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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