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The conjugation of MMS and Ab (ready for Reagent 1, R1): Chose the 3μm diameter Carboxyl MMS and use the carbodiimide cross-link technique activate the MMS with EDC and NHS in different concentration, then linked mice anti-HBsAg-IgG and mice anti-HCcAg-IgG on them.

磁微粒的活化及其与抗体的连接:选择粒径3μm的羧基化磁微粒,采用碳二亚胺共价交联法,在活化剂EDC与NHS的不同浓度组合条件下活化磁微粒,选择最适的活化剂浓度组合。

Methods Transmission electron microscope was used to observe the apoptotic morphology of the lens epithelial cells in 4 cases with senile cataract and in 2 mice with congenital cataract. The comparisons were made between the cataractous and normal transparent lens epithelial cells in human being and mice.

用透射电镜观察4例老年性白内障患者及2例先天性白内障小鼠晶状体上皮细胞凋亡的超微结构,并分别与同类正常晶状体上皮细胞超微结构进行比较。

Methods Male mice were killed by cervical vertebrae dislocation, the caudae epididymides were removed and squeezed to HTF medium, then add oocyte-cumulus complexes, after IVF 22~24 h, wash the embryos and selected the two-cell embryos to 0.5 d pseudopregnant ICR mice.

对于需要净化的小鼠的雄鼠,采集附睾的精子,放入HTF溶液中获能,然后加入经过超排的卵团,体外受精。20~22 h后,挑选形态正常的二细胞胚胎,在净化实验室,移植给假孕的SPF级ICR母鼠,待产仔。

In addition, experiments in vivo showed that tumor in mice growed faster after treatment of mice with chemotherapeutic drugs.

在此基础上,探讨混合肽诱导的特异性免疫与其它治疗因素联合的可能性及其机制。

These blastocysts developed in the recipient mice till delivery and the offspring were chimeric mice.

这些经注射的囊胚在受体小鼠内发育至分娩,产生的后代为嵌合体小鼠。

In A549 group and controling group 0.9% NS were injected to mice by abdomen respectively. In DDP group 1000uM cisplatine were injected to nude mice by abdomen which was inefficiency to A549DDP cells.

第二章 人参单体Rh2和顺铂抑制A549DDP细胞移植瘤生长的作用研究目的:探讨人参单体Rh2和顺铂对A549DDP细胞移植瘤的抑制作用。

Methods The compound preparation was administered intragastrically in mice, whose spontaneous activity was observed along with their tolerance of the preparation after long-term administration. The hypnotic effect of the compound was assessed by investigating the changes in the pentobarbital sodium-induced sleeping. The compound was tested for its antiseizure effect in mice with pentetrazole-induced clonic and tonic convulsion. Diazepam was used as the standard control in all experiments.

利用光电计数法观察灌胃给予复方酸枣仁油栀子油对小鼠自发活动的影响,以及长期使用药物对小鼠耐受性的影响;利用延长戊巴比妥钠睡眠时间实验和戊巴比妥钠阈下催眠剂量实验,观察复方酸枣仁油栀子油的催眠作用;利用戊四氮诱发小鼠阵挛性、强直性惊厥观察复方酸枣仁油栀子油的抗惊厥作用;本实验用地西泮进行平行对照实验,并进行了组方设计和实验。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基-4-苯基1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)制备成年小鼠PD模型,通过单侧纹状体定位注射GDNF,取中脑黑质节段,做连续冠状石蜡切片,结合免疫组织化学染色方法,观察各组酪氨酸羟化酶(tyrosine hydroxylase,TH)、钙结合蛋白(calbindin D28k,CB)的表达,光镜观察并细胞计数,统计学分析。

The results were listed as follows:"Cumiansan" had certain promotion and enhancement to the thickness of foot-plate in DTH, CD4+/CD8+T cell ratio in peripheral blood of the normal and the immunosuppressive mice as well as the normal mice's blood serum TNF-a content, the ultra-fine powder remarkably surpassed the commonly cribble.

结果表明,&促免散&对正常和免疫抑制小鼠足趾变态反应、外周血CD4~+/CD8~+T淋巴细胞亚群的比例以及正常小鼠的血清TNF-α的含量均有一定的促进和提高作用,但与粉体的粒度和剂量有关,微粉低剂量即可达到与粗粉中高剂量相当的效果。

Methods: The methods of the increase of blood capillary permeability of the abdominal in mice induced by injection of acetic acid and the swelling of the ear in mice caused by crotin were used for antiinflammatory experiments, aspirin and hydrocortison were respectively used as the positive control;...

小鼠随机分组,抗炎实验用醋酸诱发小鼠腹腔毛细血管通透性增高和用巴豆油诱发小鼠耳肿胀,阳性对照分别为阿斯匹林组和氢化可的松组;镇痛实验采用醋酸诱发小鼠腹痛和用热板诱发小鼠足痛,阳性对照为盐酸吗啡组。

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The Best Laid Plans Of Mice And Marionettes
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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。