查询词典 mice

Superovulated C57BL/6J female mice were sacrificed at 0.5 dpc and injected purified BAC into male pronuclei.

将由超排卵作用受精后0.5天的C57B/6J母鼠牺牲，并注射纯化后的重组MLL基因至精核中。

Then, the two recombinant genes were microinjected into the male pronuclei of fertillized mouse eggs for generating transgenic mice harbouring the introduced genes, respectively.

进而将它们分别导入小鼠受精卵雄原核，作成相应的转基因小鼠，则发现它们都失去了表达特性。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

And the mouse prions were able to change the hamster proteins into a new kind of prion that infected both healthy hamsters and mice.

老鼠朊蛋白能把仓鼠蛋白变为一种新的朊蛋白，这种新的朊蛋白能感染健康的仓鼠和老鼠。

Methods This study was carried out in 2 groups of preterm mice.

将受孕SD大鼠随机分为实验和对照组。

After selecting a highly potent strain of poxvirus that was able to traffic to tumors when administered intravenously to mice the authors engineered the virus such that it would target only specific cancer cells -- those with increased expression of a protein known as E2F and/or activation of signaling downstream of a protein known as EGFR.

在选择高度特异性痘状病毒后才能有效的到达肿瘤生长地方，然后通过静脉途径给老鼠。这些病毒都是研究人员设计好的以至于只能到达特异性肿瘤细胞，像那些增加表达蛋白E2F和信号下游活化蛋白EGFR。

After selecting a highly potent strain of poxvirus that was able to traffic to tumors when administered intravenously to mice the authors engineered the virus such that it would target only specific cancer cells -- those with increased expression of a protein known as E2F and/or activation of signaling downstream of a protein known as EGFR.

以毒攻毒：设计病毒，攻击肿瘤一种新的治疗癌症的方法正在开发中：用病毒感染肿瘤杀死癌细胞留下正常细胞不被伤害。这些病毒以病毒疗法而著称。在一项新的研究当中，David Kirn 和他在旧金山Jennerex生物疗法同事已经描述出利用新病毒疗法后老鼠和兔子抗肿瘤效应的发展。在选择高度特异性痘状病毒后才能有效的到达肿瘤生长地方，然后通过静脉途径给老鼠。

For instance, the recent outbreak of monkeypox in the U.S., the Centers for Disease Control, banned ALL personal and commercial imports of rodents, such as squirrels,rabbits, rats, porcupines, and mice.

因为最近在美国爆发的流行疫病&猴盒&，美国疾病控制中心禁止了所有来自非洲的个人和商业的啮齿类的进入，比如非洲的松鼠、兔子、老鼠、仓鼠、荷兰猪等。

The results from RNA differentiation analysis showed that, after feeding YN1, YN2, YN3, YN4 and YN5 to mice, the band of c73 was in the range of 100 to 200 bp, and only existed in at 3, 4, 5 and 7, 8, 9, 10, and not in 1, 2 and 6. Accordingly, we concluded that neutral polysome of YN3, YN4 and YN5 could cause different effects on mouse's gene.

RNA之分化展示结果发现，c73此条band 代表小鼠经分别各予破布子之中性多糖YN1、YN2、YN3、YN4、YN5灌胃给药后，其RNA分化表现之不同，并发现c73此条分化展示的band ，其大小介於100~200 bp 之间，且可看出c73只出现在3、4、5与7、8、9、10，而不出现在1、2及6，因此推测破布子的中性多糖YN3、YN4、YN5对小鼠之基因表现有不同影响。

Wenxing consistently adheres to the principle of "trict Management,All Member Participation,Quality First,Users Foremost" and follows the business philosophy of excellent quality,highly active and pursue excellence,thus outdoing its counterparts in terms of technology,production scale and administration.

文兴一贯秉承&严格管理、全员参与、质量第一、用户至上&的企业方针，遵循优质、高效和追求卓越的经营理念，从而实现在技术上、规模上、管理上处于同行领先地位。

The left atrium of the guinea pigs was innervated by the two afferent system.

豚鼠左心房受心脏的两个传入系统的双重支配。