查询词典 mice

To make the transgenic mice: We purified the gene fraction nestin-hsp68-EGFP and injected it into pronucleus of mouse zygotes to generate the transgenic mice.

1。转基因小鼠的获得：纯化基因片段nestin-hsp68-EGFP，用显微注射方法把基因片段注射到受精卵的雄原核中，获取转基因小鼠。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法，将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法，制备hbv转基因小鼠，用pcr，elisa，荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

Its effects on breast milk secretion of puerperal mice and swimming time of mice were observed.

结果产后大鼠及急性失血小鼠Hb和RBC明显降低，经养血生乳口服液治疗后，动物Hb和RBC显著升高，母鼠在产后第4天和第10天的泌乳量明显升高，小鼠游泳时间明显延长。

RESULTS: The aqueous extract of Pulsatilla Chinensis Regel reduced the micronucleus rate of bone marrow PCE cell in mice,and increased SOD activity and T-AOC in mice serum.

结果： 白头翁水提物能显著降低环磷酰胺所诱发的微核率、提高小鼠血清超氧化物歧化酶活性、增强总抗氧化能力，与阳性对照组比较，差异均具有统计学意义(P.05)。

The left carotid artery was expose and placed over an ultrasonic flow probe.The brain flow volume was recorded with an ultra-sonic volume meter.The pursiness time of mice was observed to evaluate the effect of tianma xingnao capsule on cere-bral ischemia and anoxia.Methods The permeability of normal blood-cerebral barrier was investigated in mice.

目的 应用二道血流速度流量仪超声探头测定大鼠颈总动脉，以反映云南省昭通制药有限公司提供的天麻醒脑胶囊对脑血流量的影响；方法记录小鼠断头后张口喘气时间，观察天麻醒脑胶囊的抗脑缺血缺氧作用；小鼠尾静脉注射Evans溶液，测定脑组织Evans的吸光度以反映药物的血脑通透性。

Results of the study showed that both aqueous extract of whole plant or root of M. coromandelianum Gurcke at doses of 10, 20 mg/kg, po equivalent to the crude plant could lower rabbit fever (P ＜0.05, P ＜0.01), decrease mice writhing (P ＜0.01), inhibit ear edema (P ＜0.05), and decrease the permeability of the blood capillary in abdominal cavity of mice (P ＜0.01). These results suggested that M. coromandelianum L.

结果表明：赛葵全草10、20 mg/kg ig给药能降低发热家兔的体温，延长小鼠的痛反应时间，减少扭体鼠数和扭体次数，20 mg/kg抑制鼠耳的肿胀及抑制小鼠腹腔毛细血管通透性；赛葵根10 mg/kg ig给药能降低发热家兔的体温，抑制鼠耳的肿胀，降低小鼠腹腔毛细血管通透性。

Methods:A total of 84 rds mice were randomly divided into 2 groups, with 42 mice in each group.

rds新生小鼠 84只，随机分为实验组和对照组，每组42只小鼠。

Result: In total, 3264 fertilizated eggs were injected,1779 of which developedinto 2-cell embryos after in vitro cultured.The survival rate was 54.50%.1397survived embryos were reimplanted into the oviducts of psudopregnant kunbaifemale mice.After reimplantation,16 female mice were pregnant and 85 pups wereborn. PCR analysis indicated 11 out of 85 pups weretransgenic.SouthernBlotting detection showed 1 out of 11 was transgenic.

结果：共注射了3264个受精卵，继续培养，有1779个胚胎发育至2-细胞，存活率为54.50%，将1397个存活的胚胎移植到71只假孕昆白母鼠的输卵管内，共有16只移植母鼠怀孕，得到85只仔鼠，PCR检测表明11只为转基因阳性小鼠，对其中的部分小鼠进一步进行Southern Blotting检测显示，1只为转基因阳性鼠。

HISI treatment prolong the survival time of mice in toxic condition induced by sodium nitrite and duration of electrocardiograph in trachea closed mice .HISI could obviously improve the ischemic electrocardiogram T-wave induced by Pituitrin.ST segment drifts on electrocardiogram in acute myocardial ischemia rats induced by Isoprotereno were obviously opposed by administration of HISI. The myocardial infarction areas, releasement of lactic dehydrogenase 、 creatine phosphokinase significantly increased in myocardial ischemia dogs, HISI treatment prevented these effects .

实验结果表明：苦碟子氯化钠注射液能显著延长小鼠常压缺氧环境及亚硝酸中毒情况下的存活时间，延长小鼠气管夹闭后心电消失时间；可显著改善垂体后叶素诱发的大鼠急性心肌缺血心电图T波异常；明显对抗异丙肾上腺素诱导的急性心肌缺血大鼠心电图ST段的偏移；显著改善冠脉结扎所致急性心肌缺血犬的心外膜心电图缩小其梗死心肌面积，降低心肌耗氧量，减少心肌坏死导致的心肌酶如乳酸脱氢酶、肌酸磷酸激酶的漏出。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。