查询词典 mice

Supported by: the Youth Foundation of National Natural Science Foundation of China, No. BACKGROUND: Bone marrow mesenchymal stem cells of mice are different from human and rats. The difficulties of culture, sustaining impermanency activity of BMSCs after passage and short-term effect of stem cells tracking limited the study of mice.

摘要背景：小鼠骨髓间充质干细胞培养不同于人及大鼠，培养扩展难度高，传代后干细胞活性保持时间短；又鉴于干细胞本身特点，使现有干细胞示踪方法作用时间短，这些因素均影响小鼠骨髓间充质干细胞的实验研究。

Objective To expiore the pathogenic mechanism of hemorrhagic fever with renal syndrome.Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

目的 探讨肾综合征出血热的发病机理方法 2～3天的近交系Balb/C乳鼠，脑内接早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。

ABSTRACT An experimental study has been carried out in mice to clarify whether or not antibody-dependent enhancement of hantavirus infection would take place.18 mice were intracerebrally injected 1 LD50 Chen strain virus.

为阐明肾痔疮综合征出血热能否产生抗体依赖感染增强现象，我们作了小鼠实验研究。

Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

2～3天的近交系Balb/C乳鼠，脑内接种早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。

On postnatal Day 5, wild-type mice and mice lacking alpha2A-adrenoceptor (alpha2A-KO) or alpha2C-adrenoceptor subtypes (alpha2C-KO) were randomly assigned to receive dexmedetomidine (3 microg/kg) or phosphate-buffered saline intraperitoneally. Thirty minutes after the intraperitoneal injection, the glutamatergic agonist ibotenate (10 microg) was intracerebrally injected, producing transcortical necrosis and white matter lesions that mimic perinatal human hypoxic-like lesions.

将出生后5天的野生型大鼠和缺乏α2A(α2A-KO)或α2C(α2C-KO)肾上腺受体亚型的大鼠腹膜内随机注射右旋美托咪啶(3 μg/kg)或磷酸钠缓冲液。30分钟后，脑内注射10μg 谷氨酸激动剂 ibotenate ，产生类似围产期缺氧性皮质坏死和白质损伤。

Methods BALB/c mice were anesthetized with ether and infected intracranially with 20 μl 4.5×108 plaque forming units of coxsackievirus B3. CVB3 RNA in mice brain was detected by reversal transcription polymerase chain reaction and the pathological changes of brain tissues were observed by HE staining.

BALB/c纯系小鼠分两组，实验组小鼠颅内直接注射滴度为4.5×108 pfu的柯萨奇病毒B3悬液20 μl，对照组颅内注射生理盐水20 μl，应用逆转录酶聚合酶链反应法测定脑组织中CVB3 RNA含量，HE染色观察脑组织病理学的改变。

The mortality of mice immunized with 56-47kDa fusion protein was lower than that of the mice immunized with another recombinant protein after challenge of Ot Karp.

用56一和47一kDa蛋白免疫血清分别对转染的细胞作间接免疫荧光染色，结果证明56一和47一kDa蛋白基因以及它们的融合基因均在相应的转染的细胞内表达了目的蛋白。

Methods BALB/c mice (n=176) were fed with lactalbumin and its hydrolysate, the models of oral tolerance were established, and control group A, lactalbumin group, partially hydrolysed lactalbumin group and extensively hydrolysed lactalbumin group (n=44) were divided. Meanwhile, BALB/c mice (n=83) were allergized and stimulated by lactalbumin to establish the models of food allergy, and control group B (n=41) and lactalbumin allergy group (n=42) were divided.

乳清蛋白及其水解物喂养BALB/c小鼠（n=176），建立口服耐受模型，实验分为对照A组、乳清蛋白组、部分水解乳清蛋白组、深度水解乳清蛋白组（n=44）；同时，用乳清蛋白致敏、激发BALB/c小鼠（n=83）建立食物过敏模型，实验分为对照B组（n=41）和乳清蛋白致敏组（n=42）。

METHODS In convulsive mice caused by strychnine to observe the latent period of convulsion,dying time,mortality in mice.

采用士的宁致小鼠惊厥法，观察酸枣仁生物碱对小鼠惊厥潜伏期、死亡时间和死亡率的影响。

Methods The Diaphragmatic muscle, latissimus dorsi and gastrocnemius muscles of the C57BL/10ScSn-Dmd/J and C57BL/6J mice were collected at the age of 2, 3, and 4 weeks, respectively. Each group selected 3 mice for experiments. Dmd gene were amplified and analyzed by RT-PCR.

选取2、3和4周龄C57BL/10ScSn-Dmd上标(上标 mdx/J模型小鼠及C57BL/6J小鼠各3只，分别采集膈肌、背阔肌和腓肠肌的肌肉组织，利用RT-PCR方法扩增分析Dmd基因的结构特点；采用Western bolt方法分析β-dystroglycan的表达差异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。