查询词典 mice

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

For confirmation of this ancient legend and found out the sexual improvement compounds the testosterone and NO in serum of mice were measured,the weight of sexual organs of mices was detected and the sex behaviour of mice was observed.The testosterone and NO concentration in serum of mice as indicator for screening some improving sexual compounds from Chinese chives was builted.1、The preliminary experiment of Chinese chives confirm sexual improvement functionThe sexual improvement function experiments of Chinese chives comparing with herba epimedii,herba cynomorii and radices morindae which were acknowledged as chinese herbals having sexual improvement function were carried out.The result was showed that the Chinese chives was confirmed having sexual improvement function,though its efficacy was less than herba epimedii,herba cynomorii and radices morindae.

为了证实古代医学认为韭菜的壮阳功能，揭示韭菜起温肾壮阳作用的化学物质基础，开发其功能性物质成分，本实验首先应用韭菜酒精初提液对小鼠睾酮、NO含量、性器官、性行为等指标的测定，验证韭菜是否具有改善性功能作用，然后以小鼠睾酮、NO含量等为指标对韭菜中的壮阳物质进行逐步分离和改善性功能作用筛选，以鉴定出几种改善性功能的物质。1、韭菜改善性功能的验证将韭菜与淫羊藿、琐阳、巴戟天三种有温肾补养功能的中草药进行比较，发现韭菜改善性功能作用不如淫羊藿、琐阳、巴戟天壮阳中草药的效果明显，但是韭菜还是具有显著的改善性功能作用的(P＜0.001)。

Results:All dosage groups of Tou-Yu-Tong Pills could release chronic nerve injury caused by formaldehyde solution; remarkedly inhibit the pain of mice in the formalin test and prolong the latencies of paw-licking. It could significantly diminish pain caused by glacial acetic acid ,reduce the number of the writhing in mice.To the model of migraine ,Tou-Yu-Tong Pills can improve the content of 5-HT in brain tissue of mice ,and ruduce 5-HTAA.

结果 头愈痛丸对福尔马林致小鼠慢性神经性疼痛反应，能明显减少动物舔后足的时间，改善其伤害性疼痛行为反应；对冰醋酸致小鼠扭体反应，能显著延长扭体的潜伏期，减少扭体反应的次数；对小鼠偏头痛模型，能显著升高小鼠脑内5-HT的含量，且降低5-HIAA的趋势。

METHODS: Human amnion membrane derived mesenchymal stem cells were sterilely isolated and cultured. The third passages of cells (1×109/L)(0.5 mL) were transplanted by tail venous pathway to mice. Mice in the control group were injected with an equal volume of saline, whereas mice in the normal group were left intact.

无菌条件下体外分离培养人羊膜间充质干细胞，传至第3代将细胞浓度调整为1×109 L-1，经尾静脉注入0.5 mL至细胞移植组小鼠体内；对照组经尾静脉注入同体积的生理盐水；正常组小鼠不给予任何干预措施。

ResultsESC(the extract of Solidago canadensis L.) markedly prolonged the writhing latent period and decreased the times of writhing of mice induced by ip acetic acid, while it had no obvious effect on the pain threshold value in hot plate test. ESC significantly prolonged the tussive latent period of mice, decreased the tussive times and increased phenolsulfonphthalein excretion quantity of trachea of mice. ESC was nontoxic according to the acute toxicity test.

结果首次对国产加拿大一枝黄花进行了体内药理作用研究，发现加拿大一枝黄花提取物能显著延长醋酸致小鼠扭体潜伏期，减少扭体次数；但未明显提高热板法实验小鼠的痛阈值；能显著抑制浓氨水所致小鼠咳嗽潜伏期和咳嗽次数；显著增加小鼠气管的酚红排泌量；急性毒性实验表明加拿大一枝黄花提取物无明显毒性，既不引起小鼠死亡也不影响小鼠体重正常增加。

objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera（p＜0.05）.there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

选择12例尿毒症肾性贫血未透析患者为试验组，另选正常健康体检者12例为正常对照组，将实验组、正常对照组血清分别稀释成不同终浓度，采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养，对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。结果在实验组中，与血清浓度为0的浓度组相比，与各浓度组血清共同培养的小鼠骨髓cfu-e和bfu-e的集落数均明显减少，且随血清浓度的增加，小鼠骨髓cfu-e和bfu-e的集落数逐渐减少，各浓度组间差异有显著性（p＜0.05）。结论尿毒症患者血清抑制小鼠骨髓红系祖细胞集落形成，随患者血清浓度的增加，抑制作用增强，具有剂量依赖性。

T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

Methods Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

Methods With the model of aging mice induced by D-gal, the learning memory ability of mice was measured by using Y-maze, the contents of SOD and MDA in the cerebrums were determined by xanthine oxidation or TBA colorimetry ,the variations of above indices of mice after treatment with NGD were observed.

建立 D-半乳糖致小鼠衰老模型，以 Y型电迷宫检测其学习记忆能力，分别采用黄嘌呤氧化酶法、TBA比色法测定大脑 SOD活性和 MDA含量，观察小鼠服用神经生长液后上述指标的变化。

First experiment, the effects of PCP on social behavior of mice at different dose and administration time: 60 DDY male mice of 6 months old were randomly divided into 5 groups after 2-week feeding with 12 mice in each group and fed in two separate coops.

实验1，苯西克定剂量及用药时间对小鼠社会行为的影响：选用6周龄的DDY种雄性小鼠，在饲养2周后，被抽签随机分成5组，每组12只，两笼饲养。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了