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Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果:SPG-Rg3 IC50为(155.70±0.71) mg·L-1,当SPG-Rg3浓度在37.5~600.0mg·L-1时,MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大,与对照组比较,细胞增殖受到明显抑制(P.05);MCF-7细胞的生长周期也发生变化,S期细胞数增加,明显高于对照组(P.01,G2/M期细胞数则明显少于对照组(P.01;并且在G1峰前出现明显的凋亡峰,凋亡细胞数增加。

Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2,〔53+54=(31.3±24.6)ng/mg vs. S1+S2=(5.7±4.8) ng/mg, P.01〕. Cells immunoreactive for chymase were seen throughout portal areas and intralobular sinusoidal walls, largely colocalizing with fibrosis. Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.

慢性肝炎纤维化分期重的S3和S4患者,其肝组织中Chymase浓度〔S3+S4=(31.3±24.6)ng/mg〕明显高于纤维化轻的S1和S2患者〔S1+S2=(5.7±4.8)ng/mg〕,P.01;免疫组化染色结果,Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁,其分布与纤维化部位相一致,且肝组织中Chymase浓度高的患者,其肝组织内Chymase标记的肥大细胞分布增多推测肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。

Methods 200 patients attending for colonoscopy were randomly chosen and divided into control group(n=100)and mac group(n=100).control group was given normal colonoscopy.mac group was given fentanyl (1μg /kg) and propofol (1~2 mg/s) intravenously.if the patients reached classⅴ of ramsay score,inserted the colonoscope,and maintained with propofol 5~10 mg/min.

200例行肠镜检查者随机分为mac组(n=100)和对照组(n=100),对照组行常规肠镜检查。mac组以芬太尼1 μg/kg静脉注射,继以异丙酚1~2 mg/s速度推注,ramsay镇静分级5级后行肠镜检查,术中5 ~10 mg/min速度维持mac。

The results were showed that somatic embryos can be directly re-induced from cotyledonary, which was cultured on MS medium supplemented with ZTO.5-2 mg/L, BA 1mg/L and NAA 0.2mg/L, and the induction rate was over 80%.

结果表明,MS+ZT 0.5~2mg/L+BA 1mg/L+IAA 0.2mg/L培养基可直接诱导体胚发生,诱导率超过80%;MS+BA 2mg/L+NAA 0.2 mg/L或MS+TDZ 0.01 mg/L+NAA 0.2 mg/L培养基可直接分化不定芽,出芽诱导率分别为88%、100%。

The average biomass removal rate of TN and TP of Canna indica and Cyperus alternifolius was 21.69 and 7.97 mg/, 12.31 and 5.95 mg/, respectively.

对污水中TN、TP的去除率,美人蕉达到84%、80%,伞草达到77%、72%;对TN、TP的平均生物量去除速率,美人蕉为21.69、7.97 mg/,伞草为12.31、5.95 mg/。

Experimental results show that the water velocity is that when the BV ammonia concentration of 50 mg, L - 1 simulation wastewater and 98 mg, L - 1 point of industrial wastewater, penetrate respectively C/C0 = 0.3 and 0.15, water column of natural zeolite could handle respectively; 126 BV, 43 BV Modified zeolite column can handle respectively 157 BV, water, and with 46 BV hydraulic load increases, zeolite column can reduce water treatment, The experiment was found to leak after 8 NaCl solution with zeolite desorption regeneration effect is good, low cost, regeneration of zeolite adsorption capacity decrescent, Test through dynamics analysis found that zeolite column of ammonia nitrogen adsorption fast.

实验结果表明,进水速度为2.72 BV时,通入氨氮浓度为50 mg·L-1的模拟废水和98 mg·L-1的工业废水,此时穿透点分别为C/C0=0.3和0.15时,得到天然沸石柱可处理水量分别为126 BV、43 BV;改性后沸石柱可处理水量分别为157 BV、46 BV,且随水力负荷增大,沸石柱可处理水量减小;试验还发现对泄露后沸石用8%NaCl溶液解吸再生效果好,成本低,再生后的沸石吸附容量变小;试验通过动力学分析发现沸石柱吸附氨氮速度较快。

First 27 rabbits of the experimental group were destabilizated by operated on L6、7.Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法] 取48只6个月龄日本大耳白兔,雌雄不限,体重为(2.5±0.2) kg,随机进行分组,分为对照组和实验组,对照组为21只;实验组为27只;先将实验组兔腰背部皮毛剪除,用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射麻醉后,俯卧固定于手术台上,用1%碘伏消毒手术区域,以髂嵴平对椎间隙(即L6、7)为中心,从正中取一长约4 cm纵行切口,切开皮肤及皮下组织,锐性分离,暴露棘突、椎板及上下关节突,将附着于棘突、椎板及小关节的肌肉全部分离开,然后依次切除L6、7棘上及棘间韧带,咬除第6腰椎两侧下关节突,造成椎间失稳,用无菌生理盐水冲洗切口,依次缝合各层组织;术后动物在笼中自由活动。

Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法] 取48只6个月龄日本大耳白兔,雌雄不限,体重为(2.5±0.2) kg,随机进行分组,分为对照组和实验组,对照组为21只;实验组为27只;先将实验组兔腰背部皮毛剪除,用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射####后,俯卧固定于手术台上,用1%碘伏消毒手术区域,以髂嵴平对椎间隙(即L6、7)为中心,从正中取一长约4 cm纵行切口,切开皮肤及皮下组织,锐性分离,暴露棘突、椎板及上下关节突,将附着于棘突、椎板及小关节的肌肉全部分离开,然后依次切除L6、7棘上及棘间韧带,咬除第6腰椎两侧下关节突,造成椎间失稳,用无菌生理盐水冲洗切口,依次缝合各层组织;术后动物在笼中自由活动。

Research for the antiseptic and fresh-keeping technique indicates that, the 500-fold antiseptic of 75%Tuobujing can effectively inhibit Dothiorella ribis from growing and the 200 mg.L-1 antibiotic of Qingdameisu and 160 mg.L-1 strepotmycin can effectively inhibit Pesudomonas vesicularis from growing.

防腐保鲜措施的研究表明,杀菌剂以75%托布津500倍液抑制多主小穴壳菌生长的效果最好,抗生素以庆大霉素2 000 mg/L和链霉素160 mg/L抑制泡囊假单胞杆菌生长效果最好。

One hundred and fifty 28-day-old crossbred piglets (Duroc×Landrace×Yorkshire) were randomly allocated to 5 groups (3 replicates in each group and 10 piglets per replicate) and fed with basal diets supplemented with 0, 50, 100, 200 mg/kg Zn as Zn-Gly or 3 000 mg/kg Zn as zinc oxide.

选用150头28日龄的杜×长×大三元杂交断奶仔猪,分成5组(每组3个重复,每个重复10头),对照组饲喂基础日粮,试验组饲喂分别在基础日粮中添加50、100和200 mg/kg甘氨酸锌和3 000 mg/kg氧化锌的日粮。

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