查询词典 methylation

Objective: To investigate the role of p16 gene aberrant methylation in the malignant progression of human bladder cancer.

目的：研究膀胱移行细胞癌中p16基因甲基化失活及其在肿瘤发生中的作用。

Aberrant DNA methylation is one of the major characteristics of tumor cells in addition to genetic and other epigenetic alterations.

DNA甲基化的异常是肿瘤细胞的主要特征之一，此外还有遗传的和其他表观上的改变。

Consistent with these important roles, a growing number of human diseases have been found to be associated with aberrant DNA methylation.

同这些重要功能相符合的是，越来越多的人类疾病被发现同DNA甲基化的异常有关。

Transcriptionally active genes have ___ level of DNA methylation when compared to methylation of inactive genes.

C IPTG 与 lacI 基因的产物结合，抑制它的活性E IPTG 作为β-半乳糖苷酶的别构效应物，直接刺激它的活性

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

DNA methylating inhibitor makes the transfer of the methyl group to adenine or cytosine impossible,thus the reaction of DNA methylation is inhibited,the genomic methylation level is decreased.

DNA甲基化抑制剂使甲基基团不能转移到腺嘌呤或胞嘧啶，导致DNA甲基化反应受阻，从而使基因组甲基化水平降低。

In this thesis, based on a recent genome-scale dataset of DNA methylation in human brain tissues, we developed a classifier for predicting methylation status of CpG islands using a Support Vector Machine. Nucleotide sequence contents, transcription factor binding sties and Alu repeats are used as features for the classification. The method achieves accuracy of ~85% on the brain data.

本文的主要工作就是基于人脑测得的DNA甲基化数据，挖掘DNA序列特征，用支持向量机的方法实现了预测CpG岛甲基化的目标，得到了85%的预测正确率，同时还验证了所选的三类特征对于CpG岛甲基化的指导作用，它们分别是DNA序列组合、转录因子结合位点TFBS和短重复序列Alu。

He also said, methylation has to be kept in balance; human body becomes ill due to too much or too less methylation.

甲基化指的是基因体上的化学反应，过与不及都会对人体造成不利影响。

The influences of environmental factors on the abiotic methylation were studied. The biggest production of methylmercury was found after 2 days of incubation in presence of FA. The rate of production of methylmercury depends upon temperature. In presence of FA from sediment, the biggest production of methylmercury was found under 40℃. In presence of FA from soil, production rate increases with temperature. The biggest production of methylmercury was found when solution pH was 2 and 4 in presence of FA from sediment and soil, respectively. Increase in concentrations of inorganic mercury and FA solution causes an increase in the production of methylmercury. Under irradiation with ultraviolet ray, blacklight lamp as well as natural light, methylation is stimulated, especial for irradiation of ultraviolet light.

环境条件对汞甲基化作用的影响研究得出，在底泥和土壤FA体系中甲基汞产量均在反应2天后达到最大值；在底泥FA体系中汞的甲基化在反应温度为40℃时产量最大，而在土壤FA体系中，甲基汞产量在10～60℃之间随反应温度升高而增加；底泥和土壤FA体系中汞的甲基化分别在pH值为2和4时最大；甲基汞产量均随无机汞浓度和腐殖质浓度的增加而增加；紫外线、黑光灯和自然光照射都可促进甲基汞的生成，紫外线的作用尤其明显。

The effect of low temperature treatment on DNA methylation level decrease revealed some variety differences. The DNA methylation level in stem apices of the spring radish cultivar'Duanye 13'was somewhat easy to be decreased by low temperature treatment, but that of the winter radish cultivar'Yidianhong'did not decrease when the germinated seeds were treated at 10℃ for 10 days.

萌动种子低温处理降低DNA甲基化水平的效果在品种间存在一定差异，春性品种'短叶13'其DNA甲基化水平比较容易被降低，而冬性品种'一点红'的萌动种子在10℃下处理10d尚不能降低其DNA甲基化水平。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。