查询词典 methylation

Trends of DNA methylation frequency and half DNA methylation frequency are ruleless under soil Naphthalin pollution stress in corn whole growth stage.

从玉米整个生长周期看，土壤萘污染胁迫对玉米叶片中DNA全甲级化和半甲基化频率影响均无明显规律；三个时期分别来看，土壤萘污染胁迫对玉米幼苗期和灌浆期DNA全甲级化和半甲基化频率影响较大。

Furthermore, besides considering changes in DNA methylation as mechanisms of disease, the role of epigenetics in general and DNA methylation in particular in transgenerational carcinogenesis, in memory formation and behavior establishment are brought about as mechanisms based on the cellular memory of gene expression patterns.

而且，除了考虑DNA甲基化改变作为疾病的机制外，表观遗传学一般的及DNA甲基化特别的在隔代致癌中的作用，在记忆形成和行为确立被引发作为基于基因表达模式的细胞记忆的机制。

Veratrum aldehyde synthesis of many drugs intermediates, methyl aldehyde method for raw materials in Champlain, after dimethyl sulfate methylation available to 20% sodium hydroxide as the solvent, 10-11 in the PH value of the under the conditions of synthesis; microwave irradiation is the use of microwave radiation heating to replace the traditional approach to synthesis of a product of methylation reactions, this method does not require solvents, and less by-products, but still need to use 15% sodium hydroxide to adjust pH.

藜芦醛是许多药物合成的中间体，甲基化法以香兰醛为原料，经过硫酸二甲酯甲基化可得，以20%氢氧化钠为溶剂，在PH值为10-11的条件下合成；微波辐射法是利用微波辐射代替传统加热方式进行甲基化反应合成产物，这种方法不需要溶剂，并且副产物少，但仍需要用15%氢氧化钠来调节酸碱度。

Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测，同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

Results The DNA was obtained from PPJ through a nasopancreatic tube. Aberrant p16 methylation and K- ras gene mutation were detected in the same samples of PPJ. Sensitivity,specificity,positive predictive values,negative predictive values and accuracy of HE staining for pancreatic cancer were 40%,100%,100%,45.4% and 60.0%,respectively. Of the 20 cases of pancreatic cancer,K- ras gene mutation was detected in 14 (70%) and the p16 gene was shown to be methylated in 7 (35%). Of the 8 cases of chronic pancreatitis,K-ras gene mutation was detected in 2 (25%). Of the 2 cases of mucinous cystoadenoma of pancreas,K-ras gene mutation was detected in 1 (50%). Aberrant methylation of p16 was not detected in pancreatic juice samples from patients with chronic pancreatitis and mucinous cystoadenoma of pancreas.

结果 所有胰液标本均成功抽提出DNA，30例胰腺疾病病人胰液标本同时进行了K-ras基因突变和p16基因启动子区5′CpG岛甲基化检测，其中20例胰腺癌病人胰液中K-ras基因突变率为70%（14/20），p16基因甲基化率为35%（7/20），8例慢性胰腺炎中K-ras基因突变率为25%（2/8），2例胰腺囊腺瘤病人中K-ras基因突变率为50%（1/2），慢性胰腺炎和胰腺囊腺瘤病人胰液中无p16基因甲基化。

Subsequently,enol methylation of P in basic condition completed the synthesis of malyngamide O.Applied this route to the synthesis of malyngamides Q and R,the chiral unsaturated acid was connected with the vinyl chloride part by amidation,then the resulting product was converted into the corresponding aldehyde,followed by aldol condensation of the aldehyde with the chlorotitanium enolate of pyrrolidone to complete the skeleton of malyngamides Q and R,then the synthesis of them was completed via enol methylation in acid condition.

该合成路线拓展应用到malyngamides Q和R的合成时，首先用酰胺键将手性不饱和脂肪酸和烯氯片段连接，再转化成相应的醛，与乙酰基吡咯酮进行TiCl_4催化的aldol反应即得到malyngamides Q和R的骨架结构，再经过酸催化的烯醇甲基化反应完成了Q和R的全合成。

In addition, DNA methylation profiles segregated patients with CEBPA aberrations from other subtypes of leukemia, defined four epigenetically distinct forms of AML with NPM1 mutations, and showed that established AML1-ETO, CBFb-MYH11, and PML-RARA leukemia entities are associated with specific methylation profiles.

此外，根据DNA甲基化的不同可将CEBPA基因畸变与其他亚型的白血病区分开来，并定义出四个不同表型的伴NPM1基因突变的急性髓系白血病，并且表明AML1-ETO， CBFb-MYH11，和 PML-RARA 三种白血病类型与特定的甲基化有关。

Takeshi Nagasaka, M.D., of the Department of Gastroenterological Surgery and Surgical Oncology at the Okayama University of Graduate School of Medicine, and colleagues analyzed methylation of the RAS腾讯QQ2 and 腾讯QQRP2 gene promoter腾讯QQrom 788 primary gastric and colorectal tissue specimens to determine whether methylation patterns could act as stage-dependent biomarkers of gastrointestinal tumorigenesis.

Takeshi Nagasaka医学博士来自冈山大学医学研究所胃肠外科和肿瘤外科部门，他和同事一起检测了788例早期胃癌和结直肠癌病人的组织样本中的RAS腾讯QQ2和腾讯QQRP2两个基因启动因子的甲基化现象。以确定甲基化模式可否作为胃肠道肿瘤发生的阶段性生物标记物。

Influence on corn DNA methylation frequency and half DNA methylation frequency is stronger under soil Naphthalin pollution in seedling and grouting stage, this is other mechanism of siol Naphthalin pollution stress.

这可能是土壤萘污染胁迫影响玉米生长发育的一种机制。

Seven of them have not been reported.Their structures were confirmed by IR,~1HNMR,MS,and element analysis. 4.Meanwhile,the green methylation procedure of imides was developed by using dimethyl carbonate as methylation reagent.A series of imides,for example,phthalimide,3,4-dibromo-maleimide and succincide were used for substrates.The effects of DABCO(1,4-diazabicyclo [2.2.2]octane)and DBU(1,8-diazabicyclo[5.4.0]undec-7-ene)as a catalyst in this kind of reactions were investigated.2-Bromo-3-(1-methylindolyl-3-yl)-1-methyl-1H-pyrrole-2,5-dione was prepared by using dimethyl carbonate,which further expanded the use of dimethyl carbonate,as a green agent in organic synthesis.

在以硫酸二甲酯为甲基化试剂对3，4-二溴马来酰亚胺进行N-甲基化反应合成3，4-二溴-N-甲基马来酰亚胺的同时，以碳酸二甲酯为甲基化试剂，对3，4-二溴马来酰亚胺、邻苯二甲酰亚胺、琥珀酰亚胺等酰亚胺类化合物的N-甲基化反应进行了研究；考察了1，4-二氮杂双环[2.2.2]辛烷，1，8-二氮杂双环[5.4.0]十一碳-7-烯、碳酸钾和四丁基溴化铵等作为催化剂对碳酸二甲酯N-甲基化反应的反应时间和收率等的影响，并应用碳酸二甲酯为甲基化试剂，合成了N-甲基-3-溴-4-(1-甲基-吲哚-3)-马来酰亚胺。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。