查询词典 methylation

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

Dna was extracted from 12 frozen tissues and 35 paraffin embedded tissues, then treated by sodium bisulfite and pcr amplified by specific primers for methylated versus unmethylated dna.

将提取的dna经亚硫酸盐处理后，用甲基化特异性pcr（methylation specific pcr，msp）方法检测blu和arf基因启动子的甲基化状态。

The gene chip detecting methylation changes of 3 CpG in promoter region of p16 gene was constructed by designing a pair of probes which contain one methylated and one unmethylated probes. This pair of probes was used to detect 3 consecutive sites of CpG island in p16 gene.

设计1组特殊荧光标记探针以构建1种检测p16基因启动子区3个CpG位点甲基化改变的芯片，特殊荧光标记探针包括1对非甲基化探针和甲基化探针，检测相邻的3个CpG位点甲基化的程度。

For The Methylation Assay, the readout is called beta, which is calculated from the fluorescent signal ratio of Cy3 to Cy 5, where all green signal means unmethylated and all red, fully methylated.

甲基化分析的结果称作β，是以荧光信号Cy3于Cy5之比计算得出的结果，如果全部都是绿色信号，表示未甲基化，而全为红色则表示完全甲基化。

ResultsThe rate of methylation of p15 gene was detected 37.8% in the tumor tissues and naive in the normal uro...

结论p15基因在膀胱移行细胞癌组织中的甲基化率较高，其异常高甲基化在膀胱癌的发生、发展中具有重要作用。

Promoter methylation of hTERT is a regular event in uveal melanoma.

葡萄膜黑色素瘤中 CpG 岛甲基化的启动子：通过启动子超甲基化使肿瘤相关基因失活是各种肿瘤发展过程中的一种普遍核外遗传现象。

Taken together, these results indicate that Hypb is required for embryonic vascular remodeling and provide a tool to study the function of H3K36 methylation in vasculogenesis/angiogenesis.

我们的这项研究结果证明HYPB基因在血管发育中具有重要作用，并为下一步研究H3K36三甲基化修饰在血管发育中的作用机制提供了一个良好的模型。

Methods The method of methylation and microwave synthesis method of microwave radiation Veratryl aldehydes, synthesis of these two methods compare the yield of the product and the fundamental nature, analyzing the advantages and disadvantages of both methods.

采用甲基化的方法和微波微波辐射的方法合成藜芦醛，比较这两种方法合成产物的产率及基本性质，分析两种方法的优劣。

And MSAP with 24 primer was used for analysis in Yesso scallops with orange/white muscle, it was showed that there were 46 loci which had differences in the degree of methylation between the population with orange muscle and the white, among of which 18 were methylated or semi-methlated loci, which may be related to the appearance of orange muscle.

采用24对引物组合对橘红/白色闭壳肌虾夷扇贝群体进行了MSAP分析，结果表明，甲基化程度存在差异的位点46个，其中18个位点为甲基化或半甲基化位点，推测橘红色闭壳肌的发生可能与这些位点有关，它们调控了该性状的表达。

The improved method of konjac glucomannan carboxy methylation was proposed in this paper according to the effects of deacetylation. Carboxymethyl KGM were synthesized by blending KGM with etherification agent first and then basifying and catalyzing in ethanol. Basifying first and then etherifying prepared traditional carboxymethyl KGM.

针对脱乙酰基的这种影响进行羧甲基化方法的改进，在乙醇介质中先混合醚化剂，再进行碱化催化反应制备羧甲基魔芋葡甘聚糖，并通过与传统的先碱化再醚化的KGM羧甲基改性方法比较，达到乙酰基脱出顺序上的不同。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。