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Most importantly, we demonstrate that MBD association to methylated DNA seres to identify noel targets of epigenetic inactiation in human cancer.

更重要的是，与甲基化的DNA相关的MBD起到鉴定人类癌症后生失活新靶的作用。

To further testify the high efficiency of these new reagents in the synthesis of sterically hindered peptides, we successfully synthesized the extensively -N-methylated peptide moiety of the antineoplastic and lipophilic depsipeptide Dolastatin 15 using reagent BEP and FEP. We also synthesized the highly hindered tetrapeptide segment of Cyclosporin A using reagent BEMT and BEP, respectively.

为进一步检验这类吡啶正离子型缩合剂在有空间位阻的多肽合成中的性能，我们以BEP和FEP为缩合剂，成功地合成了具有抗肿瘤活性的亲脂性酯肽Dolastatin 15分子中高N-烷基化的肽链部分；分别以BEMT和BEP为缩合剂合成了生物活性肽Cyclosporin A中空间位阻最大的四肽片断。

The standard curve was constructed by using gene chip after the methylated and unmethylated DNA were mixed at different ratio. Then treated samples of U266 cells were dotted on gene chip, obtained results were compared with standard curve to get the quantitative results.

采用基因芯片的方法，将完全未甲基化的DNA和完全甲基化的DNA混合后制成标准曲线，将U266细胞株样本处理后点在芯片上与标准曲线进行比较后得出定量检测的结果。

The promoter and partial GAG domain of CFG1 element are unmethylated, however, the partial RT domain is highly methylated.

CFG1元件的启动子和部分组特异性抗原域处于未甲基化状态，而部分RT域则处于高度甲基化状态。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

Results HPP1 gene was methylated in 37 of 52(71.2%) colorectal cancers and 12 of 21 (57.1%) adenomas cases. In contrast, HPP1 gene was unmethylated in all of the samples from healthy controls.

结果 结直肠癌粪便标本HPP1基因甲基化阳性率为71.2%(37/52)，腺瘤粪便标本为57.1%(12/21)，而正常粪便标本均为阴性。

Dna was extracted from 12 frozen tissues and 35 paraffin embedded tissues, then treated by sodium bisulfite and pcr amplified by specific primers for methylated versus unmethylated dna.

将提取的dna经亚硫酸盐处理后，用甲基化特异性pcr（methylation specific pcr，msp）方法检测blu和arf基因启动子的甲基化状态。

The gene chip detecting methylation changes of 3 CpG in promoter region of p16 gene was constructed by designing a pair of probes which contain one methylated and one unmethylated probes. This pair of probes was used to detect 3 consecutive sites of CpG island in p16 gene.

设计1组特殊荧光标记探针以构建1种检测p16基因启动子区3个CpG位点甲基化改变的芯片，特殊荧光标记探针包括1对非甲基化探针和甲基化探针，检测相邻的3个CpG位点甲基化的程度。

For The Methylation Assay, the readout is called beta, which is calculated from the fluorescent signal ratio of Cy3 to Cy 5, where all green signal means unmethylated and all red, fully methylated.

甲基化分析的结果称作β，是以荧光信号Cy3于Cy5之比计算得出的结果，如果全部都是绿色信号，表示未甲基化，而全为红色则表示完全甲基化。

And MSAP with 24 primer was used for analysis in Yesso scallops with orange/white muscle, it was showed that there were 46 loci which had differences in the degree of methylation between the population with orange muscle and the white, among of which 18 were methylated or semi-methlated loci, which may be related to the appearance of orange muscle.

采用24对引物组合对橘红/白色闭壳肌虾夷扇贝群体进行了MSAP分析，结果表明，甲基化程度存在差异的位点46个，其中18个位点为甲基化或半甲基化位点，推测橘红色闭壳肌的发生可能与这些位点有关，它们调控了该性状的表达。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。