查询词典 methods

Determination of different methods using different extraction methods and the use of melamine in samples of different volume.

不同的测定方法采用不同的三聚氰胺提取方法和使用不同的样品量。

Methods The cells were divided into 5 treatment groups（10,25,50,75 and 100 μmol·L-1 QUE）, blank control and menstruum control group. The rat C6 cells were cultivated to 1×106·mL-1 in the RPMI 1640 medium, then added into 96 holes board with various doses of QUE by 3 holes per group,and MTT assay was used to observe the proliferation of the cells treated for 24,48 and 72 h. The change of cell cycle was also observed by flow cytometry after the cells were treated with 50 and 100 μmol·L-1 QUE for 48 h. The changes of the protein P53 and Bcl-2 of C6 cells treated with 50 μmol·L-1 QUE for 48 h were detected by immunocytochemical methods.

按QUE浓度分成10、25、50、75及100 μmol·L-15个处理组和空白对照组及溶剂对照组，大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106·mL-1后，在96孔板中分别加入上述浓度的QUE继续培养，每组设3复孔，作用24、48及72 h，采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况，流式细胞术对50及100 μmol·L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析，免疫组化法检测50 μmol·L-1的QUE作用48 h 的p53和bcl-2基因产物。

By extracting the total flavonoids from Water Hyacinth with the two different methods of extraction and ultrasonic and taking Ethanol as menstruum the two extracting methods were compared;The activity of anti-oxygen of flavonoids extract was also studied.

以乙醇为溶剂，采用浸提法和超声波法提取凤眼莲总黄酮，通过单因素实验比较两种提取方法；并对黄酮提取物的抗氧化性进行了研究。

Methods (1) The device for study was determined by comparing the fluorescent images obtained by laser scanning confocal microscope and CCD fluorescent microscope respectively. The mercury-arc lamp of fluorescent microscope was studied to value its possibility to be used as the irradiation light source. Various image analyzing and processing methods were compared to determine which one was the best to be applied in this study.

(1)实验系统的建立：分别利用激光扫描共聚焦显微镜和CCD荧光显微镜采集ECV 304细胞的DCF荧光图像，并比较两者的成像质量，实验可操作性等技术指标；主要测量输出功率、功率稳定性以及光斑直径这三个指标，考察荧光显微镜的高压汞灯作为本研究中照射光源的可行性；比较并选择合适的荧光图像的分析与处理方法。

The main work of the thesis consists of summarizing the characteristics of the approximation methods,compiling the programs of approximation methods,and getting availability of every approximation method according to examples.

本文的主要工作是总结各种近似方法的特点，编写各近似方法的程序，通过算例总结出各种近似方法的适用性。

Generally speaking, the calculation methods of metaphor and metonymy can be divided into two categories: the methods based on induction

总体而言，隐喻和换喻的计算方法可以分为两类：基于约定的方法和基于推理的方法。

Two methods of Matlab and Fortran mixed programming are analyzed and introduced. The Matlab engine and the Mex files are used while the realization steps of the two methods are presented. The advantage and disadvantage are discussed with a geophysical prospecting data visualization application example.

介绍和分析了Matlab与Fortran混合编程的两种方式，即利用Mex文件和利用Matlab引擎；给出了其具体实现方法，并讨论了各自的优缺点和适用情况；给出了一个物探数据可视化的应用实例。

Methods:Automated hematologic analyzer was used for platelet count if the URI flag was not due to the effect of microcyte,these samples were also c ounted under light microscope,and the results of these 2 methods were calculated for t test.

用血液分析仪作血小板参数分析，对出现URI报警但又不是小红细胞干扰的标本作显微镜计数，把两法结果作配对t检验。

The coefficients between the sizeclasses and other quantification methods are found to vary with the particle size of the microfossil charcoal. The 202 touch pointcounts method is considered to be a better method for quantifying microfossil charcoal as it has high coefficients with other methods,smaller error and costs less counting time.

两种点接触法的微炭屑统计结果的相关系数高达0.93，反映了这两种方法所得微炭屑统计结果具有几乎相同的变化趋势。202刻点接触法由于与其他统计法的相关性都比较高，统计视域多且鉴定统计速度快，是进行微炭屑统计的最佳方法。

METHODS Microdissection technique with micromanipulator under microscope, as well as subsequent DNA extraction and polymerase chain reaction amplification methods, was employed.

通过光学显微镜直视下用显微操纵系统进行显微切割技术结合DNA提取和PCR方法，对在组织切片上获取病毒核内包涵体的来源进行分子生物学鉴定。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐