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Methods To investigate the ability of proliferation of the three lung cancer cell lines:the cell line L9981 of human high metastasis lung cancer,the cell line L9981-nm-H1 of transfected nm23-H1 gene,and the cell line L9981-pLXSN of transfected vector using MTT methods,and to examine the ability of extro-invasion of the three cell lines above using improved B...

方法应用放免法检测人高转移大细胞肺癌原代细胞株L9981,转基因细胞株L9981-nm23-H1和空载体转染细胞株L9981-pLXSN的PKA的活性,应用MTT法检测L9981,L9981-nm23-H1和L9981-pLXSN细胞株的体外增殖,改良的Boyden小实法检测L9981,L9981-pLXSN,L9981-nm23-H1这3个细胞株细胞体外侵袭力。

It is shown that preventing welding hot cracking by mechanical methods can avoid the limitation of metallurgical methods and is very effective both for solidification cracking and for liquation cracking, thus has more freedom in choice of the parent metal and filler metal.

研究表明,采用力学方法防止焊接热裂纹可以避免冶金措施的局限性,对两类热裂纹(即焊缝凝固裂纹和近缝区液化裂纹)同样有效,因此,对焊丝成分和母材的选取有较大的自由度,是一种解决焊接热裂纹问题的重要的新途径。

However, there are problems with the traditional methods such as power meter, voltage measurement of high pressure and side-current, Q-V Lissajous figure trace method. It is difficult for the traditional methods to reflect the power of the discharge device, because they can not be very accurate, convenient and practical in measuring the discharge power.

然而,传统的用于测量介质阻挡放电功率的功率表法、高压侧电流电压测量法、Q-V轨迹Lissajous图形法等在准确性、方便性、实用性等方面存在许多问题,很难真实的反映放电装置消耗的功率。

According to the features of amino acids in tea, those tea pretreatment methods mainly including extraction and lixiviation, and those popular determining methods mainly including automatic amino acid analyzer, high performance liquid chromatography, capillary electrophoresis, etc.

摘 要:在参阅1907年以来国内外关于茶叶氨基酸分析的180余篇文献的基础上,对茶叶中氨基酸的分析方法做了较系统的介绍,并针对茶叶氨基酸的特点,就茶叶样品的前处理方法(主要包括萃取法和浸提法)及目前常用的分析方法(主要包括氨基酸自动分析仪法、高效液相色谱法、毛细管电泳法等)进行了评述。

According to the features of amino acids in tea,those tea pretreatment methods mainly including extraction and lixiviation,and those popular determining methods mainly including automatic amino acid analyzer,high performance liquid chromatography,capillary electrophoresis,etc.were described too.

在参阅1907年以来国内外关于茶叶氨基酸分析的180余篇文献的基础上,对茶叶中氨基酸的分析方法做了较系统的介绍,并针对茶叶氨基酸的特点,就茶叶样品的前处理方法(主要包括萃取法和浸提法)及目前常用的分析方法(主要包括氨基酸自动分析仪法、高效液相色谱法、毛细管电泳法等)进行了评述。

And then studies on the relevant theories of distribution centers to let everyone know comprehensively, including the concept of logistics and distribution, the concept and development process of distribution center, the classification and function of distribution center, as well as its role. Lately it gives detailed description on the relevant theories of location selection, including the principle and influencing factors of location selection, as well as the steps and general methods of location selection. Finally the paper sums up several methods commonly used at present.

更多配送的概念、配送中心的概念及发展过程、配送中心的分类、功能及其作用;然后研究了配送中心选址的相关理论,包括配送中心的选址原则、影响因素、选址步骤以及配送中心选址的一般方法等部分;最后总结了目前几种常用的选址方法,并在这些理论的基础上,建立了非线性混合0-1规划类型的多配送中心选址模型。

The main works are as follows:The dissertation gives a discussion and generalization all kinds of location methods fundamental principle then compares these methods performance, realizable complexity and applied circumstance.

本文在深入研究了无线蜂窝通信系统的定位技术基础上,阐述了作者在其中几个问题上所做的工作详细讨论和总结了各种定位方法的基本原理,并且对这些方法的性能、实现复杂度和适用环境进行讨论比较。

Methods: On the basis of epidemiology of navy's injury and disease of locomotor system in our country, a series of methods were designed and their effects were observed,respectively.

根据我国海军舰员运动系统伤病的发生特点,设计一种有针对性的系列锻炼方法,并在舰员中进行应用,观察对预防运动系统损伤和疾病作用。

By using the methods of systematic analysis, Extension Set and Periphery Theory, game theory and other interrelated methods such as synthesis, comparison, logical analysis, this paper has carried the research of how to upgrade the athletic competitiveness of Chinese tennis women's double in a management vision.

本论文运用系统分析方法、可拓学理论方法、博弈论思想方法及其它相关实证研究和综合、比较、逻辑等分析方法,从管理学的视角对提高我国网球女子双打竞技水平问题进行了研究。

The different circumstances of "metaphysics" in Logical Positivism and Marxism was the result of their using different philosophical methods in order to surpass the traditional thinking methods.

"形而上学"在逻辑实证主义哲学和马克思主义哲学中的不同境遇是它们各自运用新的不同方法以图超越传统思辨方法的结果。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

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