查询词典 methods

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

The paper summarizes the earthquake damage, the characteristics of earthquake response and the antiseismic analysis method on underground structure. several methods of anti-seismic research of culvert structure are listed, and are simply compared between. finally, the methods that different design methods should be select at different design stage are suggested.

现针对受地震影响较大的浅埋涵管结构，概述了涵管结构的震害、地震反应特点、抗震分析方法，然后从地下结构的众多抗震分析实用方法中，列出了几种适合浅埋涵管结构抗震分析的实用方法，并对几种方法进行简单的分析比较，提出在不同的设计阶段应该采用不同的设计方法。

The paper summarizes the earthquake damage, the characteristics of earthquake response and the antiseismic analysis method on underground structure. Several methods of anti-seismic research of culvert structure are listed, and are simply compared between. Finally, the methods that different design methods should be select at different design stage are suggested.

本文针对受地震影响较大的浅埋涵管结构，概述了涵管结构的震害、地震反应特点、抗震分析方法，然后从地下结构的众多抗震分析实用方法中，列出了几种适合浅埋涵管结构抗震分析的实用方法，并对几种方法进行简单的分析比较，提出在不同的设计阶段应该采用不同的设计方法。

This essay explores using micosatellite DNAs to study the genetic diversity, and its major contents are as follows:The contrast among different methods of feces preservation: The methods are that feces was respectively stored in the field during collection at a low temperature after drying at 60 ℃, stored in 75% ethanol, stored in 100% ethanol and directly stored at a low temperature(in the attemperator added the biological ice bag),The result showed that DNA could be extracted successfully from the feces in a short storing period with any of these methods.

本文应用微卫星标记来研究普氏原羚遗传多样性，主要研究内容如下：一、不同普氏原羚粪便保存方法比较：通过将普氏原羚的粪便在野外分别采取60℃干燥后低温保存，75％乙醇保存，100％乙醇保存，直接低温保存。

Conclusions】 Fibrin educe have effect on micro column gel method, It can be resolve by CaCl2 centrifugalization methods for fresh blood samples and EDTA-Na2 methods for anticoagulation or non-anticoagulation blood samples, we recommend to use these methods in clinical.

用CaCl2离心法对新鲜抗凝血和用EDTA-Na2法对抗凝和非抗凝血均能很好地解决纤维蛋白析出对微柱凝胶实验的影响，值得在临床推广应用。

Based on analyzing the methods and defects of traditional instruction evaluation, this paper explained the principles , evaluation methods and process of information-based instruction evaluation, and then compared them in seven aspects, such as purpose of evaluation, the constituter of the evaluation criterion, the concern about the education resource, the methods of evaluation , the ability that students obtain, the integrate of evaluation and instructional course, the concern about the environment etc.

本文从分析传统教学评价方法及缺陷入手，阐述了信息化教学评价的原则、方法及过程，并从评价目的，评价标准的制定者，对学习资源的关注，评价的方法，学生所获得的能力，评价与教学过程的整合性和对情境的关注性等七个方面对二者其进行了比较。

Through the discussion of the study history of memory, the research problems and the significance of this study were brought forward, the concept of memory was clarified, the latest research of brain science was reviewed; On this basis, memory types and its measurement, memory process and its rules were studied documentarily and generalized systematically; This research analysed emphatically and concluded general and special methods of memory, the functions, usage means and applied scopes of many kinds of scientific memory methods were explored deeply with some typical psychology experimental results; Then affects of objective factors included external environment and material characteristics, subjective factors such as purpose, attention, emotion, knowledge, confidence and so on , were examined deeply; At last, it studied and analysed the cultivation and training of memory, practical usage of scientific memory methods, and put forward educational suggestions and training measures.

首先从记忆的研究历史切入本研究的问题背景与研究意义，阐明了记忆的概念、综述了记忆脑科学方面的最新进展；在此基础上，对记忆的分类与测量、基本过程与规律进行了文献研究与系统概括；着重分析并归结出了记忆的一般方法与特殊方法，结合代表性的心理学实验成果深入分析了各种科学记忆方法的功用、具体操作方法及应用范围；深入剖析了外部情境、材料特征等客观因素及目的、注意、情绪、知识、信心等主观因素对记忆的影响；最后在先前诸研究的基础上，对记忆的培养与训练及记忆术的实际应用加以分析和研究，提出了记忆培养训练的具体措施与教育建议，并实例说明了记忆术的具体应用。

This paper summarized the clinical research development of acupuncture and moxibustion for poliomyelitis by plentiful therapy methods, which including not only the traditional methods of simple——acupuncture、 electropuncture、 acupoint injecting, but also some new therapy methods and effective acupoint.

综述了针灸治疗脊髓灰质炎的临床研究进展，其治疗方法丰富多样，除采用单纯的针刺、电针、穴位注射等传统方法外，还探索出一些新的治法及新的有效经穴。

Methods The method review analyzed 58 example patients who accepted the concha nasalis inferior partial excision methods and under the nose endoscope the plasma iatrotechnics.28 example were concha nasalis inferior partial excision methods,and 30 were routine nose endoscopes plasma iatrotechnics.

回顾性分析58例患者接受下鼻甲部分切除术和鼻内镜下等离子治疗术的结果。其中28例下鼻甲部分切除术，30例行鼻内镜下等离子治疗。

According to the physiological and psychological characteristics of primary and secondary school students, they have a fresh image of the curious things, easy to arouse strong interest, so that teachers can use multimedia teaching methods to stimulate the enthusiasm of students, teachers, classroom teaching through multimedia teaching methods and teaching content and teaching methods be combined so that students interested in boring things, so that the knowledge students involuted easy to understand, so that Discovery is the improvement of students problem-solving skills, this is the means of the use of multimedia teaching the key.

根据小学生生理和心理特点，他们对于新鲜形象的事物有好奇心，易引起浓厚的兴趣，因此，教师可以采用多媒体教学手段以激发学生的学习积极性，教师可以通过课堂教学把多媒体教学手段与教学内容、教学方法有机结合起来，让学生对乏味的东西感兴趣，让学生对繁难的知识易于理解，让学生很愉的提高解决问题的能力，这是使用多媒体教学手段的关键所在。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。