查询词典 method of synthesis

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes. An Amorpha fruticosa cDNA clone encoding UDP-glucose pyrophosphorylase, a key enzyme producing UDP-glucose in the synthesis of sucrose and cellulose, was cloned by using this method.

本论文利用此方法首次从紫穗槐中克隆了与纤维素合成前体----尿苷二磷酸葡萄糖的代谢有关的尿苷二磷酸葡萄糖焦磷酸化酶基因和与木质素合成相关的4-香豆酸：CoA连接酶(4CL)基因的全长cDNA序列，并由此推测出相应的氨基酸序列。

In the next place, comparing each appraisable method,and combining the practical instance of railway acceleration, the thesis finally selects fuzzy synthesis evaluating method that could be much more compatible.

其次，通过对各种评价方法的比较，并结合铁路提速这样一个模糊系统的实际情况，本文选用了更为合适的模糊综合评价法。

This thesis makes up the direction for Cu-Zn-Al-O catalyst, explores the perparation method and technological conditions、the reduction method and technological conditions of Cu-Zn-Al-O catalyst, investigates Cu-Zn-Al-O catalyst activity, and studies the kinetics of γButyrolactone synthesis reaction by the liquid phase dehydrocyclization from 1, 4-Butanediol on Cu-Zn-Al-O catalyst in the batch reactor.

研究了Cu-Zn-Al-O催化剂的配方、制备方法和工艺条件、活化方式和工艺条件，考察了催化剂的活性，并在全混釜反应器中研究了1，4—丁二醇在Cu-Zn-Al-O催化剂上液相脱氢环化合成γ—丁内酯的反应动力学特性。

Two methods (Bondi Mueller method and Cortese method )which need not ethyl chloroformate as material or reagent for the synthesis of sodium taurocholate are selected for comparative studies,and the technical conditions,purity and yield of products related to the two methods are compared.

选择无需用氯甲酸乙酯作原料的邦迪缪勒法和柯特斯法进行牛磺胆酸钠的合成研究。

Adopt Fmoc solid-phase synthesis method to synthesize the linear peptide,and use DCC-HOBT method to cyclize the antamananide the result of study shows: the resulting that the linear peptide is 64%,the purity is 95%;the content of Antamananide is above 25% in the products.

采用Fmoc固相合成法合成直链多肽，并用DCC-HOBT环化法合成了抗鹅膏肽，结果表明：所得的直链多肽收率为64%，纯度为95%；所得的环化产品中抗鹅膏肽的含量在25%以上

Chemical synthesis and fermentive method is the major industrial way to produce DHS, and microbial rational modification based on the development of metabolic engineering made the fermentive method more widely used.

工业上一般采用化学合成法和发酵法来生产3-脱氢莽草酸，随着代谢工程的兴起，使得更加理性改造菌株成为可能，这更加促进了发酵法的广泛应用。

Thirdly, by means of distinguishing its features, in accordance with thesetup rule, with the aboved feature of society, economy, environment, logistics industry andsystem and network, and so on, in order to achieve sustainable development as target, throughfour angles as society, economy, environment, logistics industry, select 8 index to establish it,moreover, use the AHP law obtain their weight, besides, by analyzing incompatible problembetween index and method, existed in traditional methods, in accordance with principle andfeature of extension synthesis assessment method, evaluate and optimate logistics centerplanning schemes and get an accurate result in the practical example.

再次，通过分析研究物流中心规划方案的特点，根据建立评价指标体系的原则和要求，结合前述的社会、经济、环境、行业、系统、网络等特征，以促进可持续发展为总目标，从社会、经济、环境、物流行业四个角度考虑，有选择性的选取了8个评价指标，建立了物流中心规划方案的评价体系，并应用层次分析法得出了各指标权重。另外，在分析比较传统方法存在指标体系与评价方法存在不相容等问题的基础上，根据可拓综合评价法的原理及特点，将其引入到物流中心规划方案评价的研究中，并通过廊坊物流中心规划方案评价进行了实证研究，取得了合理的评价结果。

Based on the GIS theory, using the the overlayer algorithm of hierarchical maps in GIS method, through the corresponding constraint condition, Fuzzy Similarity Nearness degree matter-element model is applied to the flood disaster moves the plan to carry on the synthesis judgment the method.

本文试图利用模糊综合评判方法，结合GIS在洪灾避迁决策的应用，利用GIS图层叠加与空间分析的方法，通过相应的约束条件，筛选出满足约束条件的约束集，利用模糊相似贴近度物元模型进行方案的评价决策。

In this thesis, the tripeptide GHK has been synthesized by liquid-phase method and octpeptide Eptifibatide by solid-phase method. Coupling agents and purification problem have been studied in the process of peptide synthesis.

本论文分别采用液相法和固相法合成了具有药用价值的三肽GHK及八肽Eptifibatide，研究了多肽合成过程中缩合剂及纯化等问题。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！