查询词典 methanol

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The effect of various solvents such as benzene, methanol and water on the reaction pathways was assessed by the polarizable continuum model.

采用极化连续介质模型研究了各途径在苯、甲醇和水溶液中的溶剂化效应。

The solvent effect of methanol on the reaction process was investigated with the CPCM method of polarizable continuum model and the supermolecular handling, respectively.

本文先后采用连续介质模型下的CPCM方法和超分子模型考察了CH3OH溶剂对反应进程的影响。

Treated with methanol and potassium hydroxide, also known as lye, the vegetable oil molecules break down.

植物油分子经过甲醇和钾的氢氧化物、也被称作灰汁的处理后就被分解。

Result: The methanol extracts and BuOH fraction of Potentilla bifurca showed strong tyrosinase inhibitory activities.

结果：光叉叶委陵菜的甲醇提取物及其正丁醇萃取物显示较强的酪氨酸酶抑制作用。

The methanol extract conditions of Potentilla anserina and their antioxidant activities werestudied simultaneously.

研究了用甲醇提取蕨麻中类黄酮的工艺条件，并对其进行了抗氧化性能的研究。

A calculation model based on artifical neural networks technology was proposed and used in the calculation of activity coefficients of methanol propanol water system.

提出了一种基于人工神经元网络的活度系数计算模型，并成功地应用于甲醇丙醇水系统的活度系数计算。

The linear relationship to detect methanol was not effected by a few amount of ethanol, acetone and propanol etc.

乙醇、丙酮和丙醇等干扰物不影响测定的线性关系，特别对水的交叉敏感相当低，可以在真实的大气条件下测定甲醇的含量。

The resonant peak shifted towards longer wavelength with the increasing volume fraction of methanol,ethanol,propyl alcohol,acetone and formaldehyde with high sensitivities and linear relations.

结果表明，谐振波长随甲醇、乙醇、丙醇等醇类化合物，以及丙酮、甲醛等蒸气体积分数的上升而向长波方向移动，具有高的灵敏度，且两者基本呈线性关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。