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The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

My company main product 10KW~16000KW's medium frequency power supply for inductionheating and 10kg~50000kg's medium frequency furnace.We also product medium frequency power supply and mechanical device with power needs for consumer special needs etc. such as chain cutter bar's heat treatment device

产品/Product：公司主要生产10KW～16000KW的各种规格中频电源和相配套10kg～50000kg中频感应电炉，以及生产特殊要求的中频感应电源和相配套的机械设备等。

The solder removing device comprises the restoring chamber(37) used to store the liquid heating medium whose temperature is above the solder's melting point, the substrate conveying machine used to convey the defectively soldered substrate in and out the solder treating pond, and the brush(45) used to rub the surface of the defectively soldered substrate immerged in the heating medium in the solder treating pond and rub off the solders which are over heated by the heating medium.

焊料移除设备(31)包括用于存储温度高于焊料的熔点的液体形式的加热介质的修复腔室(37)、用于输送有缺陷地焊接的基片进出焊料处理池的基片运输机、和用于摩擦浸入焊料处理池内的加热介质的有缺陷地焊接的基片的表面由此擦去通过加热介质过度加热的焊料的刷子(45)。

This article briefly introduced medium and small types of biomass gasification power generation technology, 1000kW stalk and paddy power plant engineering and its economic benefit analysis; it illustrated that medium and small types of biomass gasification power generation has huge developing potential and marketing competence in our country; and it analyzed the importance of medium and small types of biomass gasification power station in rural electrization

本文简要介绍了中小型生物质能气化发电技术、1000kW秸秆和稻壳发电厂工程及其效益分析；指出中小型生物质能发电在我国具有很大的发展潜力和市场竞争力；分析了中小型生物质能发电厂对实现农村电气化的重要作用。

Ma Yun has those who experience global economic depression is sensitive, more no less than walks on the medium and small businesses that knows the amount is huge now Bao Bing, although we are impossible that the hope does the emancipator of medium and small businesses by Alibaba, but might as well must help his word as instantly with the early-warning that protects civilian battalion economy and medium and small businesses.

马云有感受全球经济衰退的敏感，更知道数量庞大的中小企业现在正如履薄冰，虽然我们不可能指望由阿里巴巴来做中小企业的救星，但不妨把他的话作为当下亟须帮助与保护民营经济与中小企业的预警。

When the microspores were cultured in the medium with 16% sucrose for 3 days, and then added an equal volume of medium with 13% sucrose, the embryoid induction frequency was enhanced greatly, up to 3.7 times of that cultured all time in the medium with 13% sugar.

如果在16%蔗糖培养基中培养3天后，添加等体积的13%蔗糖培养基，能够大大提高胚状体的诱导频率，为仅用13%蔗糖培养基培养的3.7倍。

A Medium Term Note is a debt note that usually matures in 5-10 years, but the term may be as short as one year or as long as 50 years. They're normally issued on a floating basis such as Euribor +/- basis points. When they are issued in a Euro-currency, they are called "Euro Medium Term Notes".The notes are usually connected to a Medium Term Note Program, which is a funding program used by issuers to receive bond debt funding on a regular and continuous basis.

中期票据实际上常常与中期票据项目联系在一起，中期票据项目是一种发行人可以在某一核准额度内自主持续发行债券的融资项目，这种项目的优点是赋予发行人更大的灵活性，一旦监管部门核准该发行人某一发行额度，该发行人可以在相当长的时间内(1-2年)自主选择发行时间和期限在债券市场上融资，尽管该债券称为"中期债券"。

You will come in May between June, between choice section shorter give birth to half lignification branch in those days, from base the ministry cuts dirty to insert spic, topmost reservation comes 3 pieces 5 flocculus, if branch is too long, yi Ke bite off carries a tip on the head, cuttage medium can use humus soil of orchid mud, high mountain, vermiculite, pearlite or Huang Xin earth to wait, thick about 15 centimeters reach 20 centimeters, the catchment layer that lower berth reachs 8 centimeters 7 centimeters; medium after in season is wet, in will inserting spic to insert matrix, deepness of the be buried that insert spic makes an appointment with the 1/2 that grows for spic, a few cuttage also can choose the family full-dress medium generation makes big flowerpot cuttage seedbed.

你可于5月至6月间，选择节间较短的当年生半木质化枝条，自基部切下作插穗，顶端保留3片至5片小叶，若枝条过长，亦可截去顶梢，扦插介质可用兰花泥。高山腐殖土。蛭石。珍珠岩或黄心土等，厚约15厘米至20厘米，下铺7厘米至8厘米的排水层；把介质喷湿后，将插穗插入基质中，插穗入土深度约为穗长的1/2，家庭少量扦插也可选用大花盆盛装介质代作扦插苗床。插后加盖塑料薄膜保湿，温度控制在25℃至30℃之间，加设荫棚遮挡阳光，或挪放于树荫下，始终保持扦插基质湿润，东鹃约过1个月即可生根，而西鹃则要60天至70天才能生根。9月后可减少遮荫时间，并追施稀薄尿素液一次，浓度约为0.2%，10月下旬即可上盆。

Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。

When the strain grew in the medium containing glucose(40 g/L) as carbon source with tomato juice(3.6 mL/L), gingili oil(1.6 mL/L), Na2CO3(6 g/L)and phosphate buffer, at 28 ℃ with illumination for 120 h, its cell biomass, and carotenoid yield and content could reach 65.57 mg wet cell per mL medium, 9.896 μg per mL medium and 150.9 μg per gram wet cell, respectively.

菌株在以葡萄糖为碳源，添加番茄汁（3.6 mL /L）、芝麻油（1.6 mL/L）、Na2CO3（6 g/L）和磷酸缓冲盐的液体培养基中28 ℃振荡培养5 d，每mL培养液细胞生物量湿重达到65.57 mg，类胡萝卜素产量达到9.896 μg，菌体每g湿重细胞胡萝卜素含量为150.9 μg。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了