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METHODS: Femur and tibia were obtained from Wistar rats for primary culture of BMSCs. MSCs at passage 15-20 were firstly incubated in preinduction medium, for 24 hours, and then incubated in induction medium containing α-MEM, 2% dimethyl sulfoxide and 200 μmol/L butylated hydroxyanisole.

取Wistar大鼠股骨和胫骨，进行骨髓间充质干细胞原代培养，传至第15~20代融合状态的间充质干细胞置于预诱导培养基中培养24 h后，换用诱导培养基。

It was found the freshly prepared meat extract medium was the best medium for cultivation of Propionibacterium sp.

经由实验结果得知，新鲜猪肉汁培养基是丙酸杆菌最佳的培养基。

The results indicated that 67-V medium supplied 30g/L sucrose, 200 g/L potato extract, 100 mg/L 〓 and 800 mg/L 〓 wasbest for the growth of protocorm when 100 ml liquid medium was put in 250 ml flaskand inoculum was more than 6 g/flask.

结果表明，有利于原球茎生长的最佳培养基组合为：67-V基本培养基+30g／L蔗糖+200 g／L马铃薯提取汁+100 mg／L〓+800 mg／L〓，采用250 ml三角瓶加入100 ml培养液，接种量大于6 g／flask，可获得较大的生物量。

With the protocorms of C. grandiflorium as inoculated materials and MS as basic medium, different combinations of growth regulating substances with different concentrations and different hormone proportions were designed to discuss the effects of different medium on the protocorm propagation, bud induction and rooting of C. grandiflorium and the effects of different hormone matching on the formation of roots.

方法］以大花蕙兰的原球茎为接种材料，以MS为基本培养基，设计不同生长调节物质浓度的组合、不同激素配比，探讨了不同培养基对大花蕙兰原球茎增殖、诱导芽及生根的影响和不同激素配比对形成根的影响。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者，具有显著降低污染率的效果分别为60%及50%；克服内生菌污染而建立初代无菌培养之腋芽培植体，则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者，芽体再生率最高70%-75%，惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个；另在醣种类及浓度与抗生素试验，显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基，显著降低污染率至20%；但未污染之培植体，形态发生潜能低至40%以内，可反应生长之培植体芽体形成数也仅达1-1.2个之间，再生芽体之生长也显现抑制现象。

On the base of practical transformer substation main connection, electric components and system circuit model are first formed in the paper; analyze vacuum arc characteristic and medium resume are analyzed, then, according to vacuum chamber physical process of switching AC arc, resume process are divided into three stages: sheath development, neutral particles diffusion and static characteristic, with that, calculating model of medium resume strength is erected and applied in the computing of vacuum circuit breaker interrupting transform overvoltage; In the paper, Saber software is used to simulate a whole switching process; As follows, overvoltage characteristic of vacuum circuit breaker switching no load and load transformers, kinds of influence factors are analyzed in detail; At last, vacuum circuit breaker switching capacitance group overvoltage formative causes and electrode refining for lessening reignition are discuss.

本文针对中低压变电站的实际主接线建立各电气元件及系统的电路模型；在真空电弧的特点及间隙的介质恢复特性上，依据真空断路器灭弧室分断电弧的物理过程，将真空电弧熄灭后的恢复过程分为鞘层发展、中性粒子扩散和静态特性三个阶段，建立了相应的介质恢复强度的计算模型，应用到切除变压器操作过电压的计算中；采用Saber仿真软件模拟计算真空断路器切除变压器操作过电压；并分别对真空断路器切除空载及负载变压器操作过电压的特点与影响操作过电压的各种因素做了详细的分析；最后简要分析了真空断路器开断电容器组过电压的形成原因及为减少重燃的电极老炼措施。

On the base of practical transformer substation main connection,electriccomponents and system circuit model are first formed in the paper;analyze vacuum arccharacteristic and medium resume are analyzed,then,according to vacuum chamberphysical process of switching AC arc,resume process are divided into three stages:sheathdevelopment,neutral particles diffusion and static characteristic,with that,calculatingmodel of medium resume strength is erected and applied in the computing of vacuumcircuit breaker interrupting transform overvoltage;In the paper,Saber software is used tosimulate a whole switching process;As follows,overvoltage characteristic of vacuumcircuit breaker switching no load and load transformers,kinds of influence factors areanalyzed in detail;At last,vacuum circuit breaker switching capacitance groupovervoltage formative causes and electrode refining for lessening reignition are discuss.

本文针对中低压变电站的实际主接线建立各电气元件及系统的电路模型；在真空电弧的特点及间隙的介质恢复特性上，依据真空断路器灭弧室分断电弧的物理过程，将真空电弧熄灭后的恢复过程分为鞘层发展、中性粒子扩散和静态特性三个阶段，建立了相应的介质恢复强度的计算模型，应用到切除变压器作过电压的计算中；采用Saber仿真软件模拟计算真空断路器切除变压器作过电压；并分别对真空断路器切除空载及负载变压器作过电压的特点与影响作过电压的各种因素做了详细的分析；最后简要分析了真空断路器开断电容器组过电压的形成原因及为减少重燃的电极老炼措施。

On the base of practical transformer substation main connection,electriccomponents and system circuit model are first formed in the paper;analyze vacuum arccharacteristic and medium resume are analyzed,then,according to vacuum chamberphysical process of switching AC arc,resume process are divided into three stages:sheathdevelopment,neutral particles diffusion and static characteristic,with that,calculatingmodel of medium resume strength is erected and applied in the computing of vacuumcircuit breaker interrupting transform overvoltage;In the paper,Saber software is used tosimulate a whole switching process;As follows,overvoltage characteristic of vacuumcircuit breaker switching no load and load transformers,kinds of influence factors areanalyzed in detail;At last,vacuum circuit breaker switching capacitance groupovervoltage formative causes and electrode refining for lessening reignition are discuss.

本文针对中低压变电站的实际主接线建立各电气元件及系统的电路模型；在真空电弧的特点及间隙的介质恢复特性上，依据真空断路器灭弧室分断电弧的物理过程，将真空电弧熄灭后的恢复过程分为鞘层发展、中性粒子扩散和静态特性三个阶段，建立了相应的介质恢复强度的计算模型，应用到切除变压器操作过电压的计算中；采用Saber仿真软件模拟计算真空断路器切除变压器操作过电压；并分别对真空断路器切除空载及负载变压器操作过电压的特点与影响操作过电压的各种因素做了详细的分析；最后简要分析了真空断路器开断电容器组过电压的形成原因及为减少重燃的电极老炼措施。

What small and medium-sized enterprises need is economic and effective management,so we can't reoccupy large enterprise management mechanism to set in the management of small and medium-sized enterprises.

中小企业需要的是经济而又有效的管理，不能再用大企业的管理机制来套在中小企业的管理上。

I was preparing liquid bacteria medium, after the short-time CoQ10 group conversation, I was feeling a little fine, but in the afternoom as the fermentation liquid media was cooled after 121 degree centigrade's heated, when the pH electrode is press out in the force of high pressure, that means I should reprepare the bacteria medium again.

我还是象往常一样配制培养基，本来心情还是很好的，可是当发酵液快降温的时候，pH电极被压力压了出来，晕！

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。