查询词典 medium

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育，并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响，构建了L.caseiYBQH2-14的代谢网络模型，分析了分批发酵不同阶段的代谢通量，同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in Brunfelsia latifolia , an experimentation was established in which stem segments and a series of different medium were employed.

以茎段为材料，采用不同培养基，研究确定适合二色茉莉组织培养的培养基类型，植株再生的可能途径，以及适宜的培养条件。

Kefir manufactured by goat milk powder had significantly higher medium chain fatty acid than by fresh goat milk. This could be explained by the higher fat content in goat milk powder. Addition of growth promoters decreased the level of medium chain fatty acids. By comparing both growth promoters, adding peptide had significantly lower caproic acid and caprylic acid than isomaltooligosaccharide did.

羊乳克弗尔中链脂肪酸之研究结果显示，羊乳粉制作的羊乳克弗尔的羊油酸、羊脂酸及葵酸含量均比鲜羊乳的含量高：克弗尔的中链肪酸随著添加物之不同而有所改变，整体来说，以不添加任何生长促进物的对照组其C6、C8和C10脂肪酸含量为高，添加肽类组最低。

The isolate grew well in R2 liquid medium and could pass through 0.22 μm and 0.45 μm filtrate membranes. The colony was grain-like in solid medium. Through electron microscopy, the isolate YY0801 exhibited helicity during their exponential growth phase. The isolate YY0801 was able to ferment glucose and D-fructose and to catabolize arginine, but did not to hydrolyse urea. The isolate was resistant to ampicillin (2000 U/mL).

分离菌株在R2液体培养基中生长良好，能通过孔径为0.22 μm、0.45 μm的微孔滤膜；在R2固体培养基上呈颗粒状菌落；在对数期呈典型的螺旋状；能利用葡萄糖、D-果糖作为碳源；能强烈代谢精氨酸；不能利用尿素，在含氨苄青霉素钠(2000 U/mL)的R2液体培养基中生长良好。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

METHODS: The Chelerythrine was used as the experimental group with concentrations ranging from 24.4μg/ml to 390.6μg/ml prepared with BHI broth medium with contained 2% glucose, and BHI culture medium was used as the control group.

采用二倍稀释法，用含2%蔗糖的脑心浸萃液肉汤琼脂液体培养基，将白屈菜红碱稀释为从390.6μg/ml到24。

Chiral medium is a new absorbing material, which is one kind of biisotropic medium.

手征媒质是一种新型的吸波材料，它是双各向同性媒质的一种。

The fact that wastewater has higher chromaticity removal efficiency in acid medium than in basic medium indicates that H+ ions play an important role in chromaticity removal ,possibly by a mechanism of deoxidation in which H+ ionsreact with the organic compound of pollutants in wastewater and cause the chain rupture of chromophoric or anxochromic groups from the organic compound molecules.

事实已经废水色度去除效率高，比在酸性介质中显示基本氢离子色度去除起到了重要作用，可能的机制脱氧其中氢离子的反应有机化合物废水和污染物链条断裂造成anxochromic色或团体从有机化合物分子。

In light of Fresnel formula the author analyses the features of linearly polarized light reflecting on the surface of apparent medium,discusses the relationship between the circumrotation in the plane of vibration,changes of the light intensity and the angle of incidence,the orientation of the plane of vibration,and addresses the relationship between the swing,phase and the incident angle and medium.

自然光在分界面上反射的一些特点在许多光学教材[1，2]上都进行了介绍，本文根据菲涅耳公式和菲涅耳定律，分析线偏振光在透明介质分界面上反射时的一些特点，讨论反射光的振动面所发生的旋转及光强度的变化与入射角、振动面的方位之间的关系；进一步讨论在全反射情况下，反射光的振

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。