查询词典 medium

32 U/mL enzyme activity can be detected in medium after fermentation. By optimizing the zymotic medium and fermentation conditions, recombination B. subtilis can express as high as 110.02 U/mL of Alkalineβ-mannanase. Cycle of fermentation needs 5 days(4 days is needed in induced expression ), then enzyme activity maintained at the highest level, less than 5 % enzyme activity was lost in next 2 days.

为了继续对碱性β-甘露聚糖酶进行高表达研究，试验采用了芽孢杆菌发酵培养基来进行产酶研究，初步诱导酶活达到29.32 U/mL，通过对发酵条件的优化，该重组枯草芽孢杆菌的产酶水平有了大幅度的提高，最高可达110.02 U/mL。

For 480 days of decomposition, the decomposition rate was highest in soil with medium fertility and lowest in soil with low fertility among three type of fertility. The decomposition of water soluble factions and lignin were promoted in medium fertility soil. However, alcohol-benzene soluble fraction, and semi-cellulose and cellulose fraction were decayed acceleratedly in soil with high fertility. Furthermore, more humic substance was formed and energy states of decayed corn stalk was highest in soil with high fertility.

腐解480 d，3种肥力间比较发现，玉米秸秆在中等肥力田块上矿化率最高，低肥力田块上的最低；中等肥力土壤能够促进玉米秸秆中的水溶性有机组分和木质素的分解，而高肥力土壤能够促进苯–醇溶性组分和半纤维素、纤维素分解，并有利于腐殖物质的形成，而且腐解物的能态最高。

Rice saccharification medium as selective medium for Acetobacter, easy to operate and with good effect, is better than other selective mediums.

结果表明，以大米糖化汁培养基作为醋酸菌的筛选培养基，易操作，筛选效果好，优于其它的醋酸菌筛选培养基。

Result According to the result of identification, TF413 belonged to Alcaligenes faecalis. The optimum temperature for it degrading triazophos was 32℃, and the optimum primary pH value was 7.0, the optimum liquid volume in flask was 50 ml medium in 100 ml erlenmeyer flask, the optimum medium was nutrient broth, the inoculation amount had no obvious effect on degradation effect of TF413 on triazophos.

结果］经鉴定，TF413菌株为粪产碱杆菌，其降解三唑磷的最适温度为32℃、最适初始pH值为7.0，最适装液量为100m1三角瓶中装50ml培养基，最佳培养基为营养肉汤，接种量对TF413降解三唑磷的效果无明显影响。

The corrosion resistance performance of cladding material is far better than ordinary steel in most of the acidity medium, alkalescent medium and water.

研究了这种材料的耐腐蚀性能，发现它们在大多数酸性介质、碱性介质和水中的耐腐蚀性远远好于一般金属材料。

More information about their structure and function relationship will be meaningful in understanding the molecular mechanism of ion pump and conformation alteration during ion transport and sensory transduction. In this paper, we report screening strains from samples collected from Ebi lake and Altun moutain Aqik lake, Xinjiang Province, and got 60 clonies from them. We choose 8 of them for further investigation and confirmed that they all belong to the Halobacteriaceae genus and through comparasion of several minute diffrerence medium we had the optimal medium for their growth.. Through this procedure we had established a set of methods for screening clonies from wild environment.and isolation and purification of these archaerhodopsin. Furthermore we used PCR method to identify their DNA sequence.

我们从新疆艾比湖和海拔4250米的阿尔金山阿其克库勒湖中采集到水样、土样和冰样，从中分离出60个单菌落，挑选了8个单菌落进行活化和扩大培养，确证了它们属于嗜盐菌属；确定了它们的最适生长条件；建立了一套完整的从野生环境样品中筛选嗜盐菌菌株的完整方法；摸索出了提取它们中的古紫质蛋白的方法；进行了初步PCR鉴定，为后续研究奠定了良好基础。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The results showed that the red pigment belonged to anthocyanidin, it was soluble in water, stable against heat treatment, light, acid and sugar concentration, tolerant to reduction, but sensitive to alkaline, oxidation and Fe(superscript 3+). The research also showed that acid medium is favorable for extraction. The pigments have more general stability in acid medium and have high ufility value.

结果表明：该色素为水溶性多酚类花色素，在不同的pH环境下显示不同的颜色；其耐热性、耐光性、耐酸、耐糖、耐还原性较好，但耐氧化性、耐碱性较差；Fe(上标 3+)对色素有破坏性；该色素的提取宜在酸性介质中进行，酸性条件下其综合稳定性较优，有较高的利用价值。

The results showed that the red pigment belonged to anthocyanidin,it was soluble in water,stable against heat treatment,light,acid and sugar concentration,tolerant to reduction,but sensitive to alkaline,oxidation and Fe~(3+). The research also showed that acid medium is favorable for extraction.The pigments have more general stability in acid medium and have high ufility value.

结果表明：该色素为水溶性多酚类花色素，在不同的pH环境下显示不同的颜色；其耐热性、耐光性、耐酸、耐糖、耐还原性较好，但耐氧化性、耐碱性较差；Fe3+对色素有破坏性；该色素的提取宜在酸性介质中进行，酸性条件下其综合稳定性较优，有较高的利用价值。

The asymbiotic germination indicated that the medium Hyponex No.1 was optimum for PLB formation when supplemented with potato juice, and optimum for PLB differentiation when supplemented with banana juice. Fresh sprouts were induced from the explant of the shoot tip cultured on the MS medium in the presence of BA 3.0 mg/L and NAA 0.2 mg/L.

结果表明：在花宝1号附加土豆的培养基上萌芽效果最好，原球茎发生率较高；分化阶段以花宝1号附加香蕉的培养基分化效果最理想，幼嫩茎尖在MS基本培养基上附加BA 3.0 mg/L与NAA 0.2 mg/L上可诱导出新芽。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。