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GCJ8 activate medium, preculture 2 days, 5 X Ca concentration in preculture medium and coculture medium, inoculation I mineture,4mg li1 AgN0 and 0.7% P\P, delay selection 5 days will obtain the highest transformation frequenc Using 'pingyitiancha'shoot apex, mediated by A.tum as above system, the HVAIgene, was transformed into pingyitiancha and obtained Kam resistance rerzeneration plants, it is confirmed that 6 plants of them is transformation plants by identified by PCR and dot blot

按照上述体系,以平邑甜茶茎尖分生组织为受体,将HVA1基因导入平邑甜茶中,获得了具有Kam抗性的再生植株,经PCR与点杂交鉴定,证实有6株为转化植株。

Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果]大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好;适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA,诱导出芽率可达130%;适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA,试管苗移栽后成活率为78%。

The 1/2 MS medium supplemented with 1.0 mg·L-1 BA and 0.1 mg·L-1 NAA was very beneficial to the protocorm differentiation and propagation of D.candidum .The highest protocorm propagation index was obtained from the medium containing the activated carbon.The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg·L-1 NAA.

结果与结论:添加20%马铃薯液有利于种胚的萌发,种胚萌发率为79.37%;BA 1.0 mg.L-1和NAA 0.1 mg.L-1最有利于原球茎增殖,培养基中添加2%活性碳原球茎增殖倍数高;NAA有助于试管苗的生根,不同含量NAA以0.5 mg.L-1时试管苗平均根数最多和平均根长最长。

The germination test of Sporocarps of Microlepia hancei prantl had been done, the callus can be induced from the delicate stem of Microlepia hancei prantl; The inducement and proliferation medium of callus was MS+6-BA2 mg/L+NAA0.2 mg/L;the optimal combination of hormones of buds differentiation was MS+6-BA 0.4 mg/L; the effect of MS medium in radication culture was better than 1/2MS, the roots of plants were all efficiently induced in MS medium without reference to adding IBA、NAA or IBA+NAA, the rate of radication was high; the effect of seedling cultured in the mixture of perlite and turf soil was better than perlite、garden soil and river sands.the mixture of perlite and turf soil has the excellences such as ventilate、water conservation 、nourishment and so on,the survival rate was higher and seedling substance was also hale.

试验进行了华南鳞盖蕨孢子果的萌发试验,并利用幼嫩茎段进行诱导愈伤组织;愈伤组织的诱导与增殖培养基为MS+6-BA2 mg/L+NAA0.2 mg/L;其芽分化最佳激素组合为MS+6-BA 0.4 mg/L;生根培养中MS基本培养基比1/2MS效果好,MS基本培养基不论附加生长素IBA、NAA或IBA+NAA,均能有效地诱导植株生根,生根率高;假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙,具有通气、保水、营养等优点,假植成活率高,苗势健壮。

Add the resazurin sodium solution, mix, and place the medium in suitable vessels that provide a ratio of surface to depth of medium such that not more than the upper half of the medium has undergone a color change indicative of oxygen uptake at the end of the incubation period.

加入刃天青钠溶液,混匀,并将该培养基置于适当容器中,该容器应为培养基提供特定的面积-深度比,以使在培养期末表明氧气摄入的变色部分不超过培养基的上半部分。

Secondly, referring to the present study on the connotation of the medium-income group and its measurement of dividing, the paper puts forward the basis and method of the sexenary division of the citizens and defines of the medium-income group as a group of people whose income is just lower than the millionaire and billionaire, Similar to the "middle class" popular in the western countries in economy and politics. As tar as the measurement is concerned, the paper sets up the measurement considering both the quality and quantity on the basis of income. From the point of view of quality, the medium-incomes should be able to pay for the real estate, life-lone education, medical care and luxurious goods all or mostly by their personal income, inheritance or loan form banks. In addition, they should be capable of keeping their income rising steadily.

其次,结合当前学术界对中等收入者的内涵及其划分标准的研究现状,本文提出城乡居民六等分的依据和方法,将本文研究视野中的中等收入者概括为一类仅次于亿万富翁和百万富翁的高收入者,在经济特征上,类似于西方世界流行的&中产阶级&;在其划分标准上,确立了以收入作为划分不同群体的依据,衡量标准采用质与量相结合的方法,从质上看,中等收入者的收入应能全部或绝大部分通过借贷、继承等方式支付房产、教育、医疗、高档消费等支出,并具有可持续增长的收入能力。

Maximum sporification occurred when the fungus were cultured on the oats medium at the inoculation concentration of 1×107 cfu/ml and RH 65%; on PPDA medium at the inoculation concentration of 1×106 cfu/ml and RH 85%; or on the corn powder and yeast soaking flour medium at the 1×108 cfu/ml and RH 75%.

分析了绿僵菌菌株在3种不同培养基上培养时其产孢量的2个影响因素(初始接种浓度和相对湿度),得出菌株在3种不同培养基上培养时须分别配以适宜的接种浓度和相对湿度,以获取最大产孢量(燕麦培养基:1×107cfu/mL,65%;PPDA培养基:1×106cfu/mL,85%;玉米粉酵母浸膏培养基:1×108cfu/mL,75%)。

The steady state kinetics of the lignin peroxidase catalyzed oxidation of veratryl alcohol by H2O2 in a sodium bis(2-ethylhexyl) sulfosuccinate/isooctane/toluene/water reverse micellar medium was studied and a comparison with the corresponding aqueous medium was made to understand the effect of the reverse micellar medium on the catalytic mechanism and kinetic parameters.

为了理解木素过氧化物酶在反胶束介质中的催化性能与动力学行为,本文以LiP催化过氧化氢氧化藜芦醇反应为模型反应,研究了2-乙基己基琥珀酸磺酸钠/异辛烷/甲苯/水反胶束中上述模型反应的稳态动力学并与水介质做了比较。

When the porous solid skeleton is approximately taken as a micropolar medium and the fluid in the pores a point medium, the elastic pragmatic wave equations in a saturated porous micropolar medium are obtained from the results of the micropolar theory and those of Biot's pragmatic wave theory.

利用微极理论和Biot波动理论的研究成果,把饱和土中多孔固体骨架部分近似地视为微极介质,孔隙中的流体部分视为质点介质,获得饱和多孔微极介质的弹性波动方程。

Assume that medium A has an audience of 100.000persons and medium B, 90.000, if 40.000 of the persons exposed to medium B are also members of A' s audience, then buying the two media yields an unduplicated audience of 150.000---not 190.000, duplication leads to frequency, which is the number of times the target audience is exposed to a message within a given tome period--- usually four weeks.

假定一个具有中等及中等100.000persons观众乙、90.000、如果人接触了40.000乙同属一个中型的观众然后买了两产量媒体地表观众---不150.000190.000,导致重复频率这是多少次接触对象是一个特定的讯息--通常四星期内托。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力