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The present paper carries on the raise first to the camptotheca acuminata roots of the hair, then in the use chloroform extraction camptotheca acuminata culture medium the camptotheca acuminata alkali and so on effective component, and examines the law with HPLC to examine its effective component, the examination discovers the camptotheca acuminata secretion in the culture medium secondary metabolite besides the camptotheca acuminata alkali, the hydroxyl camptotheca acuminata alkali, the white birch fatty acid, but also includes other effective components.

本论文先对喜树发根进行培养，然后使用氯仿提取喜树培养基中喜树碱等有效成分，并用HPLC检测法检测其有效成分，检测发现喜树分泌在培养基中的次生代谢产物除了喜树碱、羟基喜树碱、白桦脂酸外，还含有其他有效成分。

Thepresent paper carries on the raise first to the camptothecaacuminata roots of the hair, then in the use chloroform extractioncamptotheca acuminata culture medium the camptotheca acuminataalkali and so on effective component, and examines the law withHPLC to examine its effective component, the examination discoversthe camptotheca acuminata secretion in the culture medium secondarymetabolite besides the camptotheca acuminata alkali, the hydroxylcamptotheca acuminata alkali, the white birch fatty acid, but alsoincludes other effective components.

本论文先对喜树发根进行培养，然后使用氯仿提取喜树培养基中喜树碱等有效成分，并用HPLC检测法检测其有效成分，检测发现喜树分泌在培养基中的次生代谢产物除了喜树碱、羟基喜树碱、白桦脂酸外，还含有其他有效成分。

The medium used is a modified artificial medium for growing Volvox globartor, devised by Provasoli and Pintner (1959). The process of culture is divided into three steps:(1) preliminary culture;(2) pure culture and (3) expand culture.

我们采取过渡性的初步培养、纯化培养和扩大培养三步法，利用光强度800—1200勒克斯，每日照光10—12小时，一般室温在15—22℃(冬季不低于9℃，夏季不高于28℃)下，细胞已完全能正常的生长、繁殖而被培养成功。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

The composition of the lactic acid bacteria growth medium,centrifuging condition of the bacterium cells and selection of cryogenic medium were studied .

对乳酸菌生长培养基的组成、菌体细胞的离心条件和冷冻干燥介质选择等工艺条件进行了研究。

Routine method,medium diluting method,centrifuging and centrifuging medium diluting method were for the verification of methodology on five traditional chinese medicine.

随着药品剂型的日益发展和药品标准的不断提高，及时有效地进行药品微生物限度检查是摆在药检工作人员面前的重大课题。

In the study of isospin-dependent nucleon-nucleon elastic crosssections in nuclear medium we find the medium correction of nucleon-nucleon scatteringcross sections is also isospin dependent,〓 depends on the baryon density weakly and 〓 depends on the baryon density significantly.

用QHD-Ⅱ模型研究了介质两体截面的同位旋相关性，研究结果表明截面的介质修正也是同位旋依赖的：中子-质子介质截面对核密度的依赖很弱，而中子-中子的介质截面对核密度的依赖很强。

The longest lifetime component in PALS results is ascribed to the annihilation of positronium in cavities of PS sample. It is found that 80% of the positrons implanted in PS film have formed positronium atoms. PALS results show that the lifetime of ortho-Positronium has small value when the sample was in oxygen gas medium compared with those in other medium.

正电子湮没寿命谱测试结果表明，样品中存在长达40ns的电子偶素湮没成分，并且进入多孔硅膜层的正电子约有80%形成电子偶素，具有非常高的电子偶素产额；在氧气气氛下，由于气体导致o-Ps发生自旋转化猝灭是使多孔硅样品中电子偶素寿命缩短的主要原因。

After purification, cells were cultured in Ham's F12 medium or Ham's F12 hormonally defined medium for different time respectively, and the specific expression of cytokeratin 18 (CK18) and alpha-1-fetoprotein in the primary cultures was characterized by indirect immunofluorescence method. Cell growth and proliferation was checked by MTT method.

观察培养细胞的形态特征，免疫荧光法检测胚胎肝细胞角蛋白18 (Cytokeratin 18， CK18)和甲胎蛋白(alpha-1-fetoprotein， AFP)表达， MTT法检测细胞活力和增殖能力。

At the initial observation of leakage, judge on the leaking part of the foundation, and then manually set car observation, if the leakage were no significant changes in static and dynamic sealing ring has questions; such as disk when leakage can be concluded that there is significant change are moving , static friction ring problem exist; such as leakage of medium along the jet axis, the actin ring seal problem exist mostly, leakage to the surrounding medium or from the water spray cooling holes in the leakage, the many for the static seal ring failure.

在初步观察泄漏量、判断泄漏部位的基础上，再手动盘车观察，若泄漏量无明显变化则静、动环密封圈有问题；如盘车时泄漏量有明显变化则可断定是动、静环摩擦副存在问题；如泄漏介质沿轴向喷射，则动环密封圈存在问题居多，泄漏介质向四周喷射或从水冷却孔中漏出，则多为静环密封圈失效。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。