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The asymbiotic germination indicated that the medium Hyponex No.1 was optimum for PLB formation when supplemented with potato juice, and optimum for PLB differentiation when supplemented with banana juice. Fresh sprouts were induced from the explant of the shoot tip cultured on the MS medium in the presence of BA 3.0 mg/L and NAA 0.2 mg/L.

结果表明:在花宝1号附加土豆的培养基上萌芽效果最好,原球茎发生率较高;分化阶段以花宝1号附加香蕉的培养基分化效果最理想,幼嫩茎尖在MS基本培养基上附加BA 3.0 mg/L与NAA 0.2 mg/L上可诱导出新芽。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育,并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响,构建了L.caseiYBQH2-14的代谢网络模型,分析了分批发酵不同阶段的代谢通量,同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in Brunfelsia latifolia , an experimentation was established in which stem segments and a series of different medium were employed.

以茎段为材料,采用不同培养基,研究确定适合二色茉莉组织培养的培养基类型,植株再生的可能途径,以及适宜的培养条件。

Kefir manufactured by goat milk powder had significantly higher medium chain fatty acid than by fresh goat milk. This could be explained by the higher fat content in goat milk powder. Addition of growth promoters decreased the level of medium chain fatty acids. By comparing both growth promoters, adding peptide had significantly lower caproic acid and caprylic acid than isomaltooligosaccharide did.

羊乳克弗尔中链脂肪酸之研究结果显示,羊乳粉制作的羊乳克弗尔的羊油酸、羊脂酸及葵酸含量均比鲜羊乳的含量高:克弗尔的中链肪酸随著添加物之不同而有所改变,整体来说,以不添加任何生长促进物的对照组其C6、C8和C10脂肪酸含量为高,添加肽类组最低。

The isolate grew well in R2 liquid medium and could pass through 0.22 μm and 0.45 μm filtrate membranes. The colony was grain-like in solid medium. Through electron microscopy, the isolate YY0801 exhibited helicity during their exponential growth phase. The isolate YY0801 was able to ferment glucose and D-fructose and to catabolize arginine, but did not to hydrolyse urea. The isolate was resistant to ampicillin (2000 U/mL).

分离菌株在R2液体培养基中生长良好,能通过孔径为0.22 μm、0.45 μm的微孔滤膜;在R2固体培养基上呈颗粒状菌落;在对数期呈典型的螺旋状;能利用葡萄糖、D-果糖作为碳源;能强烈代谢精氨酸;不能利用尿素,在含氨苄青霉素钠(2000 U/mL)的R2液体培养基中生长良好。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml,经percoll液分离后密度梯度离心,〓/ml密度接种培养,观察原代细胞的贴壁、增殖状况,细胞长满瓶底后进行传代培养;传至第三代细胞,重新消化后以500转/分钟轻度离心5分钟,〓/ml接种,改用化学限定培养基代替完全培养基培养,倒置显微镜观察细胞生长情况及形态变化,甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖,免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成,RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

METHODS: The Chelerythrine was used as the experimental group with concentrations ranging from 24.4μg/ml to 390.6μg/ml prepared with BHI broth medium with contained 2% glucose, and BHI culture medium was used as the control group.

采用二倍稀释法,用含2%蔗糖的脑心浸萃液肉汤琼脂液体培养基,将白屈菜红碱稀释为从390.6μg/ml到24。

Chiral medium is a new absorbing material, which is one kind of biisotropic medium.

手征媒质是一种新型的吸波材料,它是双各向同性媒质的一种。

The fact that wastewater has higher chromaticity removal efficiency in acid medium than in basic medium indicates that H+ ions play an important role in chromaticity removal ,possibly by a mechanism of deoxidation in which H+ ionsreact with the organic compound of pollutants in wastewater and cause the chain rupture of chromophoric or anxochromic groups from the organic compound molecules.

事实已经废水色度去除效率高,比在酸性介质中显示基本氢离子色度去除起到了重要作用,可能的机制脱氧其中氢离子的反应有机化合物废水和污染物链条断裂造成anxochromic色或团体从有机化合物分子。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。