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The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Antimonate calcium precipitates in cell wall of hyphae grown for 5h in pH 4. 0 medium appeared less than in pH 6. 8 medium. And it was proved that pH in medium gradually reduced with the cultivation time during cultivation of hyphae.

在pH 4.0介质中培养5h后的菌丝，顶端细胞壁的焦锑酸钙沉淀比pH 6.8介质中培养的菌丝明显减少，并证实菌丝在生长过程中培养介质pH随培养时间的延长而逐渐下降。

In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.

研究以凤仙花无菌苗胚芽段作为外植体，经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花，在60d左右实现了凤仙花离体培养再生植株并在试管内开花。

Have the skill of relevant work way and grade letter, if CAD is three-dimensional software, medium / advanced numerical control, medium / advanced electrician, medium / advanced benchwork preferential

具备相关工作方向的技能及等级证书，如CAD三维软件、中/高级数控、中/高级电工、中/高级钳工等优先

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

The optimal determining conditions were obtained: adding quantity of a monolayer medium 15ml, the suspension concentration of Micrococcus luteus 109 cfu/ml, the adding quantity of Oxford Plate 100μl, agar concentration 1%, the concentration of Na2HPO4 1％, and the pH of the medium adjusted to 7.0. The medium was optimized by single factor and the orthogonal experiments on the basis of the centrifugate of distiller's grains. The optimal composition was obtained as follows: sucrose 0.6, peptone 0.1, yeast extract 0.2, K2HPO4?

以管碟法为基础，对影响Nisin效价测定的各种主要因素进行了分析，并得出了Nisin效价测定的最佳条件：采用单层培养基（每个平板加量为15ml），检测培养基中Na2HPO4·12H2O浓度改为1％，菌悬液浓度109cfu/ml，不加吐温20，琼脂浓度1％，培养基pH7.0，牛津杯加液量100μl ，30℃培养24小时。

Import and extension the main body of a book is superintended in order to decide a judiciary , independence of the judicature brand-new visual angle boundary and medium, the depth discusses an independence of the judicature and the medium superintends the contradiction under legal rules and system currently in effect, and by bringing forward the train of thought resolving the independence of the judicature and the medium superintending contradictious new to contradictious analysis.

本文以全新的视角界定司法、司法独立和媒体监督的含义和外延，深刻论述司法独立与媒体监督在法律规定和现行体制下的矛盾，并通过对矛盾的分析，提出解决司法独立与媒体监督矛盾的新思路。

Import andextension the main body of a book is superintended in order todecide a judiciary , independence of the judicature brand-newvisual angle boundary and medium, the depth discusses anindependence of the judicature and the medium superintends thecontradiction under legal rules and system currently in effect, andby bringing forward the train of thought resolving the independenceof the judicature and the medium superintending contradictious newto contradictious analysis.

本文以全新的视角界定司法、司法独立和媒体监督的含义和外延，深刻论述司法独立与媒体监督在法律规定和现行体制下的矛盾，并通过对矛盾的分析，提出解决司法独立与媒体监督矛盾的新思路。

An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后，分化标记物K10弱阳性表达， PAI-3呈阳性表达；培养48 h后， K10强阳性表达，而PAI-3的阳性表达明显增加， 72 h后，表达开始降低，在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质，同时， PAI-3存在终末分化KCs角质壳中。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。