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Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例,在入院当天采集深部呼吸道吸引物,用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养,然后提取深部呼吸道吸引物中病原体的DNA,采用荧光定量单PCR的方法检测肺炎支原体,并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因,扩增产物用Luminex100多功能悬浮点阵仪检测。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2-4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L-1 after their number counted.

选取2~5代的小鼠胚胎成纤维细胞,待其至对数生长期倒掉或吸掉培养基,加入含10 mg/L丝裂霉素C的细胞全培养液5 mL,置37 ℃,体积分数0.05的CO2饱和湿度温箱中培养二三小时,在6孔板中加入0.1%明胶包被,放置30 min以上,倒掉或吸掉含丝裂霉素C的培养基,磷酸盐缓冲液清洗5遍,尽量除去丝裂霉素C,加入2 mL 0.05%的胰蛋白酶消化细胞,镜下观察,当细胞间出现裂隙,细胞变圆时(约2~4 min),立即加入等量的全培养液终止消化,并用吸管反复吹打,使之成为单细胞悬液,在细胞计数板中央放置专用的盖玻片,用玻璃虹吸管吸取细胞,让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液,至盖玻片下被液体充满为止。

Studied on micropropagation of Pistacia vera showed: the suitable medium that will support excellent growth of Pistacia vera was the Murashige and Skoog medium supplemented with 2. 0 mg. L〓 6-BA and 0.05mg. L〓 NAA, but the frequency of shoot-tip necrosis which reached to 36. 4 percent in 35 days is high. Instead MS medium with Driver-Kuniyuki-Walnut medium, the frequency of shoot-tip necrosis were declined from 36. 4 percent to 9. 3 percent.

组织培养试验发现:以MS为基本培养基,添加2.0mg.L〓 6-BA和0.05mg.L〓NAA可增大繁殖系数和加速生长,但茎尖干枯率高,培养至35天时干枯率达36.4%;以DKW培养基为基本培养基进行增殖培养,35天时干枯率仅为9.3%,茎尖干枯率降低了27.1%。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果:用诱导分化液作用后,未见小的、伴有突起的放射状形态的神经细胞;抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性; C2C12细胞β Tubulin Ⅲ抗体染色阴性;上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Software of management of medium and small businesses popularizes storm is not the strategy that is aimed at a competitor, it is the medium and small businesses that we are based on 90% above completely makes without the current situation of use management software strategy consideration, invest massive free plan this to will rise to urge action revolutionarily to promoting informatization of medium and small businesses, the medium and small businesses that there is more than 40% after I hope 3 years mixes him from paper liberate above the pen come out, run oneself business with software through informatization, have quickly with in the administrative informatization weapon with large euqally preeminent company participates in globalization competition.

&中小企业管理软件普及风暴不是针对竞争对手的策略,完全是我们基于90%以上的中小企业没有使用管理软件的现状而做出的战略考虑,这次投资巨大的免费计划对促进中小企业信息化将起到革命性的推动作用,我希望三年后有超过40%的中小企业把自己从纸和笔上面解放出来,通过信息化用软件管理自己的生意,迅速拥有与中大型企业一样优秀的管理信息化武器参与全球化竞争。&

objective:to investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.methods:forty-eight female sd climacteric rats were randomly divided into 4 groups with one control group and the other low,medium and high dose groups,which were given soybean isoflavones 0 mg/kg.bw,42 mg/kg.bw,83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.the levels of sod,gsh-px and mda in serum were measured,and the ability of study and memory in each group was deterimined by adopting the law of diving platform.results:the content of serum mda in medium and high dose groups was lower than that in control group.the activity of serum gsh-px im medium and high dose groups was significantly increased,so was the activity of serum sod in low,medium and high dose groups.conclusion:soybean isoflavones do have the obvious effect of antioxidation,and improve the ability of study and memory effectively in female sd climacteric rats.

目的:观察大豆异黄酮对更年期雌性sd大鼠抗氧化作用及学习记忆的影响。方法:选用健康更年期雌性sd大鼠,随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水,实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮,连续60d。第60d取血测定血清过氧化脂质丙二醛含量、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力,用跳台实验方法测定大鼠学习记忆能力。结果:与空白对照组比较,中、高剂量组大鼠血清mda含量显著降低,低、中、高剂量组血清sod活力明显升高,中、高剂量组血清gsh-px活力明显升高。结论:大豆异黄酮具有明显的抗氧化作用,并有效提高更年期雌性sd大鼠的学习记忆能力。

The growth of plantlets with the height of 1-2 cm and 3-4 leaves was compared on MS basal medium containing various organic compounds, including banana, carrot, potato, coconut water, and tryptone. The result showed that the growth of oncidium plantlets was the best on medium containing banana, potato and tryptone altogether with respect to their plant height, leave number, root number, root length, fresh weight, and dry weight. The next better media were the control and the Banana medium, followed by the Potato medium.

另外取高约1-2公分,带3-4片叶片的分化小苗,培养於含不同浓度有机添加物(香蕉、胡萝卜、马铃薯、椰子水、及tryptone)的半量MS培养基,以探讨最适合文心兰瓶苗生长的培养基,试验结果显示,在同时含香蕉、马铃薯及tryptone三种成份的培养基生长的小植株不论在株高、叶数、根数、根长、鲜重、及乾重均较其他培养基为佳;不含添加物之1/2MS培养基、及含香蕉的培养基次之,而在含马铃薯之培养基较差。

From the ideology angle of view, combined with the characteristic of the medium dissemination, this paper expounds the "authenticity" of medium dissemination from the following three aspects:(1)"Individual viewpoint does not represent that of the station";(2)"Poets" steeple outdoes garret of parties;(3)Medium function the authenticity of medium dissemination with Chinese characteristic.

该文从意识形态的视角,结合媒介传播的特性,从三个方面对媒介传播的&真实性&进行了深入的讨论。第一,&个人观点不代表本台&;第二,&诗人的尖塔高出党派的阁楼&;第三,媒介功能-中国特色的媒介传播的真实。

Last week medium board piece quantity can have not release, much emptier both sides is relatively careful, from last week 5 go in light of situation, bibcock expression relatively grab an eye, short-term have the sign with sure look forward to, among them in inspect medium last week 5 strong harden, carry brace up medium board piece; And Sai Di medium last week 5 also behave active, rise 8.48%.

上周传媒板块量能尚未释放,多空双方都较为谨慎,从上周五走势来看,龙头股表现较为抢眼,短期有企稳的迹象,其中中视传媒上周五强势涨停,提振了传媒板块;而赛迪传媒上周五也表现活跃,上涨8.48%。

The whole-space response characteristics for three dimensional conductive body located in the floor of the mine drift either in homogeneous medium or in layered medium was simulated, with using Mur absorbing boundary condition and taking a electrical dipole source in homogeneous whole space as a initial source; the diffused regularity of the transient field in homogeneous medium at 1.9 μs and 27.6 μs,and in layered medium at 1.2 μs and 0.023 ms was discussed.

利用Mur吸收边界条件,选用均匀全空间电偶极源作为初始激发源,模拟了均匀介质中巷道底板岩层内部和层状介质中三维低阻异常体的全空间响应特性,分析了瞬变电磁场在均匀介质中1.9 μs和27.6 μs两时刻的传播规律及在层状介质中1.2 μs和0.023 ms两时刻的传播规律。

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Medium
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推荐网络例句

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台,"Mays这样说道。"

F: I think the oval shape suits you well.

我觉得这副椭圆形的可能很适合你。