查询词典 medium model

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Biot's consolidation theory was used to serve as a frame working with the nonlinear elastic model or with elasto-plastic model in the program. Nonlinear elastic model includes Duncan-Zhang model, Gong Xiannan Ko consolidation model and the model proposed in chapter 2. Elasto-plastic model in the program includes Mohr-Coulomb model, Drucker-Prager model and the bounding surface model developed in chapter 3. The program can be used in cases of plane problem and axisymmtric problem.

FOR，针对软土地基的变形特点，该程序以Biot固结理论为框架，可以进行线弹性，非线性弹性和弹塑性的有限元计算，在非线性弹性模型中包括了Duncan—Chang模型、龚晓南Ko固结模型和本文建立的非线性模型，弹塑性模型中包拓了Mohr—Coulomb模型，Drucker—Prager模型以及本文中建立的边界面模型，该程序可以考虑平面问题也可以考虑轴对称问题。

This paper presents an IFMC CAD model that consits of a geometry model and a material model, in which the geometry space acts as a base space and the material space acts as a bundle space. In this CAD model, the geometry model is based on the non-manifold model. In addition, a half-face data sturucture, which is derived from the half-edge data structure with the non-manifold feature of IFMC taken into account, is adopted to represent the geometry and topology information of the component. For the material model of IFMC, this paper focuses on the FGM component representation firstly and present a simplex-subdivision based CAD data exchange format, in which the material information is represented as a (n-1) simplex and material distributing feature is represented by the interpolation on the simplex-subdivision. Based on those, a part-building orientation optimization algorithm and an adaptive slicing algorithm for FGM component are presented in the paper. For the IFMC material model, the IFMC material information representation is divided into a meso-scale and a macro-scale representation. In the meso-scale, a concept named parameterized periodic functional meso-structure is presented as a unique form to represent the FGM (the homogeneous materials are regarded as a special FGM), the composite and the functional meso-structure material. The model of PMS is a three-tuple that contains the space state informatation, the material parameter and the material meso-scale distribution feature. The macro-scale material information representation is similar to the FGM components by interpolation of the control parameter of the periodical functional meso-structure based on the simplex-subdivision. Through an example of manufacturing-oriented IFMC CAD data processing, it is proved that the IFMC CAD model and the material information representation and process method proposed in this paper can provide a reliable data support for IFMC digital concurrent design and manufacturing.

本文将理想材料零件CAD模型建立在以几何空间为底空间、以材料空间为丛空间的结构上，使用非流形几何模型作为理想材料零件几何拓扑模型的基础，并在半边数据结构基础上，针对理想材料零件的非流形特征局限内部边界上的特点，给出了一个半面数据结构来表述零件的几何拓扑信息；对于理想材料零件的材料模型，本文先从功能梯度材料零件的信息表述与CAD数据交换和处理入手，将材料信息表述为（n-1）维单纯形，然后通过对三维几何区域的单纯剖分，以插值的方式表述零件材料分布特征；在此基础上，根据功能梯度材料零件分层制造中对CAD数据处理的要求，给出了综合考虑零件几何特征与材料特征的生长方向优化算法和自适应切片算法；而对于文中所定义的理想材料零件，本文将其材料信息表述分解到细观和宏观两个尺度进行，首先给出了细观尺度上参数化的周期性功能细结构概念，以此来统一表述功能梯度材料（单质材料作为特殊的功能梯度材料看待）、复合材料和功能细结构材料；把周期性功能细结构模型化为一个包含空间状态信息、材料构成参数和材料细观分布特征参数的三元组，以表达零件的细观材料特征；对于零件宏观的材料变化特征，则同样在几何区域单纯剖分的基础上，通过对细观尺度上周期性功能细结构控制参数的插值来完成；通过理想材料零件CAD数据处理的算例，验证了本文中理想材料零件CAD模型及材料信息表述与处理方法完全可以为理想材料零件的数字化制造提供可靠的数据支持。

This paper presents an IFMC CAD model that consits of a geometry model and a material model, in which the geometry space acts as a base space and the material space acts as a bundle space. In this CAD model, the geometry model is based on the non-manifold model. In addition, a half-face data sturucture, which is derived from the half-edge data structure with the non-manifold feature of IFMC taken into account, is adopted to represent the geometry and topology information of the component.For the material model of IFMC, this paper focuses on the FGM component representation firstly and present a simplex-subdivision based CAD data exchange format, in which the material information is represented as a (n-1) simplex and material .distributing feature is represented by the interpolation on the simplex-subdivision. Based on those, a part-building orientation optimization algorithm and an adaptive slicing algorithm for FGM component are presented in the paper.For the IFMC material model, the IFMC material information representation is divided into a meso-scale and a macro-scale representation. In the meso-scale, a concept named parameterized periodic functional meso-structure is presented as a unique form to represent the FGM (the homogeneous materials are regarded as a special FGM), the composite and the functional meso-structure material. The model of PMS is a three-tuple that contains the space stateinformatation, the material parameter and the material meso-scale distribution feature. The macro-scale material information representation is similar to the FGM components by interpolation of the control parameter of the periodical functional meso-structure based on the simplex-subdivision.Through an example of manufacturing-oriented IFMC CAD data processing, it is proved that the IFMC CAD model and the material information representation and process method proposed in this paper can provide a reliable data support for IFMC digital concurrent design and manufacturing.

本文将理想材料零件CAD模型建立在以几何空间为底空间、以材料空间为丛空间的结构上，使用非流形几何模型作为理想材料零件几何拓扑模型的基础，并在半边数据结构基础上，针对理想材料零件的非流形特征局限内部边界上的特点，给出了一个半面数据结构来表述零件的几何拓扑信息；对于理想材料零件的材料模型，本文先从功能梯度材料零件的信息表述与CAD数据交换和处理入手，将材料信息表述为(n-1)维单纯形，然后通过对三维几何区域的单纯剖分，以插值的方式表述零件材料分布特征；在此基础上，根据功能梯度材料零件分层制造中对CAD数据处理的要求，给出了综合考虑零件几何特征与材料特征的生长方向优化算法和自适应切片算法；而对于文中所定义的理想材料零件，本文将其材料信息表述分解到细观和宏观两个尺度进行，首先给出了细观尺度上参数化的周期性功能细结构概念，以此来统一表述功能梯度材料(单质材料作为特殊的功能梯度材料看待)、复合材料和功能细结构材料；把周期性功能细结构模型化为一个包含空间状态信息、材料构成参数和材料细观分布特征参数的三元组，以表达零件的细观材料特征；对于零件宏观的材料变化特征，则同样在几何区域单纯剖分的基础上，通过对细观尺度上周期性功能细结构控制参数的插值来完成；通过理想材料零件CAD数据处理的算例，验证了本文中理想材料零件CAD模型及材料信息表述与处理方法完全可以为理想材料零件的数字化制造提供可靠的数据支持。

Firstly, the course reviews the basic knowledge about matrix algebra and linear model, and introduce the BLUP for mixed linear model, then, learn the approaches to contruct and solve various animal models, including single trait animal model, repeatability model, maternal model, threshold model, random regression model, non-additive model and multiple traits model, finally, train application of animal model to animal genetic evaluation by means of a software on estimation of breeding values.

本课程首先回顾有关矩阵代数和线性模型的基础知识，介绍线性混合模型的BLUP求解方法，然后根据动物性状和动物育种资料的类型和特点，具体讲授不同种类动物模型，包括单性状动物模型、单性状重复率模型、单性状母体效应模型、单性状阈模型、单性状随机回归模型、单性状非加性效应模型和多性状动物模型等的建立和求解方法。最后，结合动物育种值估计软件，进行动物模型在动物生产性能的遗传评估中的应用训练。

This paper reviews the processes in glacier hydrological model in term of runoff generation model and runoff concentration model, analyzes the runoff generation mode from two most widely ablation model (the meteorological factors statistical model and the energy balance model), and enlarges on the runoff concentration model from three sub-models: the surface routing sub-model, the englacial hydrology sub-model and the sub-glacial hydrology sub-model.

从冰川产流和汇流两个水文物理过程出发，介绍了国内外冰川水文模型的研究进展，对目前应用最广的两类消融模型－基于气象因子的统计模型和基于物理机制的能量平衡模型进行了分析和讨论；从冰川表面、内部以及下部汇流3个方面阐述了冰川汇流模型所取得的成果。

The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明：0 1mg/L2，4 D或0 1mg/L2，4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导；在0 1mg/L2，4 D的培养基上形成的愈伤组织主要以胚状体的形式再生，而在含0 1mg/L2，4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生；再生过程中，B5基本培养基上的苗子不易生根，需要附加0 1mg/L的IBA，而在MS培养基上苗子可直接形成根。

A first recording medium (1) containing a content encrypted by a content encryption key, a portable second recording medium (2), a terminal (10) to which the first recording medium and the second recording medium can be detachably attached, a communication device (20) to which the second recording medium can be detachably attached, and a server (40) connected to a communication device.

配备存储用内容密钥加密的内容的第1存储介质(1)、可搬运的第2存储介质(2)、可装卸第1存储介质和第2存储介质的终端(10)、可装卸第2存储介质的通信装置(20)、以及连接通信装置的服务器(40)，终端将用公开钥对通信钥和持有人ID加密后的第1加密数据写入第2存储介质，安装于通信装置，并送到服务器。

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。