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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.
本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。
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Combining traditional DTH hammer with pneumatic rock drill,by reference to the rotational mechanism of rock drill,studies the rotational mechanism of DTH hammer and introduces a new type of DTH hammer that uses backstroke energy of piston to drive drill to rotate.
将传统的潜孔锤与凿岩机相结合,研究了潜孔锤孔底回转机构,介绍了一种新型的潜孔锤。
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Then injection machine is chosen, as well as the mold concrete design, including several important parts: the number of the cavity, the dividing face, designing of the injecting system, cooling system, air supplement and platoon overflows system, the outing mechanism, the mechanism for taking out the core and so on.
然后是注射机的选择,接下来是模具的具体设计,其中包括几个重要的部分,如型腔数的确定,分型面的确定,浇注系统,冷却系统,引气排溢系统,脱模机构,抽芯机构等部分的设计。
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First, CLBAC, a central load balance mechanism based on AC is proposed in this paper to increase security. Second, a distributed load balance mechanism is proposed. In DLB, each user can pre-estimate the actual load of each cell by means of virtual distributed coordination function, and then access the cell whose load is the lightest.
本文首先提出基于接入控制器的集中式负载均衡策略以提高网络安全性,然后提出分布式负载均衡策略:各用户在接入网络的时侯,首先采用虚拟的分布式协调功能预测各小区的实时负载,然后接入负载最轻的小区。
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Aimed at a changing power mechanism,a ratchet mechanism with double electric pawls,we introduce the operating principle,establish its model and optimize the important geometry parameters in its structure design in this paper.
针对一种动力转换机构———弹性双棘爪棘轮机构,介绍了它的工作原理,对其结构设计中的重要几何参数建立了模型,进行了优化,为下一步的动力学优化设计打下了基础。
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Aimed at a changing power mechanism, a ratchet mechanism with double electric pawls, we introduce the operating principle, establish its model and optimize the important geometry parameters in its structure design in this paper. This founds a basis of next step for optimum design of its structural dynamics.
针对一种动力转换机构-弹性双棘爪棘轮机构,介绍了它的工作原理,对其结构设计中的重要几何参数建立了模型,进行了优化,为下一步的动力学优化设计打下了基础。
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Group burst incidents ; early-warning mechanism ; pressure-reducing mechanism
群体性突发事件;预警机制;减压机制
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During the four years study on the project, we develop a dynamical forecast model that describing the subtropical ridge surface. A new theory on the role of the land-sea thermal difference on the formation of the Subtropical High is advanced. It is concluded that in the summer subtropics over each continent and its adjacent ocean LO, SE, CO, and a double-dominant heating from west to east compose a LOSECOD heating quadruplet. The influence of the Eurasia continent on SH varies according to the seasonal variation. From the ideal numerical simulation it is shown the important role of the Eaiuan continent and the huge topography on the mechanism of formation and mantanence of the SH during the summer. It is verified by the numerical simulation that the extension of the subtropical continent into the tropics greatly enhances the " East Asian monsoon". Through the Rossby energy dissipation theory it is verified that the deep latent convective heating induces the onset of the South China Sea monsoon over the Bay of Bengal. The relationship and mechanism between the precipitaiton over the East Asia and the Subtropicla high is illustrated by the data. It is also found the close phase lock between the east-west shift of the SH and the disturbence of the east wind belt over the tropical troposphere. The interaction between the structure and activity feature of the SH and the Meiyu over the Yangtze-Huaihe river valley is revealed from the data. The numerical simulation verified the impact of the different SH pattern on the path of the typhoon.
本项目的研究发展了描述副热带高压脊面变化的动力模型,提出了夏季副热带地区四叶型非绝热加热分布型及相应环流拼图的创新理论;明确了大陆尺度的海陆热力差异在副热带高压形成中的作用;指出欧亚地形对副热带高压和气候的影响随季节变化而变化,通过数值试验证明春季青藏高原的感热加热对亚洲夏季风的爆发地点起了&锚定&的作用,进一步证实了副热带高压断裂和亚洲夏季风的最早爆发地点发生在孟加拉湾东岸;利用Rossby波能量频散理论证明了孟加拉湾深对流潜热释放诱导南海季风爆发的机制;揭示了东亚季风降水的季节内变化与西太副高的关系及机理;揭示了西太副高与东西风带中扰动之间的相互作用过程,指出西太副高的东西活动与热带对流层上空东风带扰动和中纬度西风带扰动的移动和锁相密切相关;研究了夏季淮河流域梅雨期间副高结构和活动特征及其与江淮暴雨的相互作用;验证了不同副热带高压形态对台风路径的不同影响;通过数值试验证明了欧亚大陆和青藏高原等大地形对副热带高压形成和维持的重要作用。
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This article analyzes the price formation mechanism from three aspects in the electricity market: the interests balance among the enterprises, the optimal resources allocation, the price flexibility and the external characteristics of the industrial economy. So, we have reached a core conclusion, that is, the integrated value of the electricity is the basis of the price formation. We can negotiate the benefit distribution among enterprises gradually and vertically by introducing the flexibility cost of the power enterprises, the use of the positive and the negative externalities of electric powers, the property rights decomposition of the power resources and the value re-estimation into the mechanism of the price formation.
本文从各企业利益平衡、资源配置的最优化、弹性价格及电力产业经济外部性特征等三个方面分析电力市场价格形成机制,分析得出一个核心的结论,电力资源的综合价值是电力价格形成的理论基础,将发电企业的弹性成本,电力正负外部性,电力资源的产权分解与电力价值再估算不断地引入价格形成机制之中才能逐步地协调电力市场纵向结构间企业之间的利益分配关系。
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This thesis first re-explain the application ofgovernmental accounting according to the subject postulates of the four basis postulates, and suggest that the viewpoint of differentiating accounting recording subject and reporting subject and makes some suggestions how to establish the reporting subject in the Chinese government . Secondly, this thesis puts forward a viewpoint that we should take basis of accounting as a basic postulates of government accounting, and an analysis on the initializing mechanism and realization mechanism of governmental accounting worldwide, and last this thesis makes some suggestions on the Choice base of Chinese governmental accounting.
首先对现有四个基本假设中的主体假设在政府会计中的应用进行重新解释,提出区分记账主体和报告主体的观点以及对如何确立中国政府会计的报告主体提出建议:其次,主张将会计基础作为政府会计的一项基本假设,并结合国际社会政府会计实践对政府会计运用权责发生制和收付实现制进行了分析,提出中国政府会计基础选择的若干建议。
- 相关中文对照歌词
- Self Attack Mechanism
- Zero Mechanism
- 推荐网络例句
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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.
另一方面,更重要的是由于城市住房是一种异质性产品。
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Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.
气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。
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You rap, you know we are not so good at rapping, huh?
你唱吧,你也知道我们并不那么擅长说唱,对吧?