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In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏,匀浆、研磨,经高速离心,超速离心和蔗糖密度梯度离心,分离肝脏质膜,得到肝脏质膜的电镜切片:其次,用去垢剂TritonX-100增溶膜蛋白,利用放射受体分析法,测定细胞膜及膜蛋白与~(125)I-hGH的结合活性,均得到理想的饱和曲线;再次,将两点结合试验法与凝胶层析法结合检测到特异结合物,然后,经SDS-PAGE电泳分析,纯化后的样品呈现一条带,与标准分子量比较,得出分子量为119KD。

First, this paper presents three girders model with double cable planes which main girder and side girder is connected by rigid arms, analyzes the dynamic characteristic of Yonghe cable-stayed bridge and obtains its natural vibration modes (i.e., frequency, damping values and the mode shapes ) by ANLSIS structural analysis program and uses these parameters as dynamic dactylogram of the intact case of cable-stayed bridge. Using the passage of random as the ambient vibration ,the dynamic properties of Yonghe Bridge is measured and the modal parameter of its 15 years after is identified and used as dynamic dactylogram of the damaged case. By the finite element analysis and locale vibration test, damaged and undamaged modal parameter is analyzed and curvature mode shape, which is sensitive to damage state, is introduced as possible candidate for identifying and locating damage in the cable-stayed bridge. Selecting the branch of part measured point from modal parameter, this paper deduces the damaged index which can detect the possible damaged location of the cable-stayed bridge deck and identifies the damaged region and degree for Yonghe Bridge. For the purpose of structural condition monitoring in in-situ , from another viewpoint, this paper introduces the method called the best optimization vector to detect damaged location and degree. The accuracy and reliability of the method presented in this paper are demonstrated by static analysis and test and the healthy state of Yonghe Bridge can be evaluated.

建立了双索面通过刚臂与主梁及边梁相连的三梁式力学模型,采用ANSYS结构分析程序对永和斜拉桥进行动力分析,对其振动模态参数进行识别,以此作为斜拉桥完好状态下的动力&指纹&;采用自由交通流作为环境振源,对永和斜拉桥动力特性进行测试,识别出永和斜拉桥使用15年后的模态参数,确立了破损状态下的动力&指纹&;利用有限元分析、现场振动测试,对破损前后的模态参数进行分析,提取对破损状况较敏感的模态曲率作为斜拉桥破损诊断的参数;并从模态参数中选取部分点的分量,导出可以指示斜拉桥桥面大概损伤位置的Index损伤指标,识别出斜拉桥发生破损的区段及破损程度;考虑大型斜拉桥在线监测要求识别破损的具体位置,从另一角度提出采用最优矢量法具体确定破损发生的位置及程度;为验证本文研究成果,对永和斜拉桥进行静力分析及静力测试,对其健康状况提出可靠的评价。

And he smote Moab, and measured them with a line, casting them down to the ground; even with two lines measured he to put to death, and with one full line to keep alive.

8:2 又攻打摩押人,使他们躺卧在地上,用绳量一量,量二绳的杀了,量一绳的存留。

And he smote Moab, measured them with a line, casting them down to the ground; even with two lines measured he to put to death, with one full line to keep alive.

8:2 又攻打摩押人,使他们躺卧在地上,用绳量一量,量二绳的杀了,量一绳的存留。

Measured the basic physical parameters of unsaturated soils in Three Gorges Reservoir area; 2 measured the initial sample matric suction using the unsaturated triaxial testing system, which can be used to evaluate the unsaturated soil strength and stress; 3 the drying and moisture absorption experiments were executed using the Tempe apparatus, and got the Soil-Water Characteristic Curve, permeability coefficients of every stress level were simulated according to the parameters from the SWCC; 4 the unsaturated soil strength tests were carried out using the GDS triaxial testing system, then got the Mohr circle and shear strength parameters; the results from different stress paths were compared; meanwhile, the axial strain-stress ratio-volume strain curve of different matric suction and different radial pressure was got, as well as their relationship; The research, which provided the necessary data and reference for Three Gorges Reservoir area geologic disaster, is the basic component of Three Gorges Reservoir geologic disaster prevention and research, and experimental results will be used directly in the project.

1量测了三峡库区非饱和土的基本物性参数; 2用非饱和土三轴试验仪量测了试样的初始基质吸力,为评估非饱和土的强度和应力水平提供参考; 3用Tempe仪和体积压力板仪进行了非饱和土的干燥和吸湿试验,得到了土-水特征曲线,并根据其参数拟合出各级吸力状态下对应的渗透系数; 4用GDS三轴仪进行了强度试验,得到了Mohr圆和抗剪强度参数;并比较分析了不同应力路径下试验结果;同时得到不同吸力和不同围压下轴向应变-应力比-体应变的关系曲线和其相互关系。该研究是三峡库区地质灾害防治研究工作的基础性工作,其结果将直接应用到工程实际中,为三峡库区地质灾害提供必要的数据和参考。

A differential demodulation system of Laser Doppler is designed. Phase grating is adopt to obtain same intensity measured beams with big separative angle in the system,and by focusing the waists of two Gauss beams on the measured object with extender and collimating lens group, the smallest speckle and the superposition of plane waves are obtained.

设计了一种差动检波系统,采用相位光栅获得同光强、大夹角的测量光束,用扩束准直透镜组使两束高斯光束的束腰聚焦在被测物体上,得到最小光斑和平面波的叠加。

METHODS: Using tissue culture technique, the maximal non-toxic concentration of luteolin in swine testicle cell culture was measured, and the inhibitory effect of luteolin on TGEV was measured by cytophathogenic effect observation and crystal violet measurement. In addition, the degrees of inhibition of the luteolin on CPE of virus in cell culture were observed by changing sequence of adding the vires and the drag. RESULTS : The maximal non-toxic concentration of luteolin was 873.39 μmol/L.

采用细胞培养法,通过结晶紫摄入量测定结合细胞病变作用,观察木犀草素在不同的加药方式下,对感染TGEV的猪睾丸细胞的保护作用、结果:TGEV病毒先吸附ST细胞再加入药液,先与TGEV病毒孵育再接种于卯细胞,或药液先与细胞孵育再用病毒感染等不同方式下,木犀草素在0.84-54.50μmaol/L浓度范围内,均能明显地提高细胞的存活数。

Under different structural parameters of exit tube,including the inner diameter and the structure shape,the separation efficiency and pressure drop of the guide vane cyclone tube were measured by comparative experiments and the flow field in the guide vane cyclone tube was measured by an intelligent five-hole pilot tube.

通过对比实验测定了不同排气结构下导叶式旋风管的分离效率及压降,同时用智能型五孔球探针测试仪测定了不同排气结构的旋风管内流场分布,结合性能实验与流场测量的结果,研究了排气芯管内径及其结构型式对分离性能的影响,为旋风管结构优化和开发及其分离机理的研究提供了一定的实验基础。

When we begin to the medicine, urine protein was measured in urine volume of 24 hour the every two day. 4. Specimen collections and laboratory check: In the 21 days of pregnant rats, infants were taken which registered weight and height after aesthesia. Cr and BUN in the blood were measured.

标本收集和实验室检查:于妊娠第21天在戊巴比妥钠(50mg/kg)麻醉下,剖宫取胎,记录胎仔体重,身长;同时抽取腹腔静脉血,分离血清以待测定血尿素氮,血肌酐;取左肾部分置于10%甲醛液浸泡固定,待用HE染色、PAS染色及免疫组织化学染色;另取部分肾脏剪成体积约1cm〓大小组织块,生理盐水冲洗至发白,-70℃保存。

The trend of intranasal pressure drop at different airflows measured by CFD model was the same as that measured by rhinomanometry, while the transnasal pressure obtained by CFD model was lower than that recorded by rhinomanometry.

CFD模型计算各流量下鼻腔压降变化与鼻阻力计测量得到的压力-流量分布曲线的变化趋势一致,但压降值前者小于后者。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。