查询词典 maturation period

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

The invention relates to a method for synchronizing at least one node of a bus system, which is operated with a predeterminable system clock period, whereby a local clock period and a reference clock period are given for the at least one node, and the reference clock period is synchronized with the system clock period. A local clock period, which is synchronized with the system clock period, of the at least one node is produced whereby linking the local clock period with a splitting factor, whereby the splitting factor represents a ratio of the reference clock period to the local clock period. The splitting factor for synchronizing the local clock period with the system clock period is adapted by adding or subtracting a adaptation value.

用于同步至少一个总线用户的方法，所述的总线系统用可预定的系统周期运行，由此为该至少一个用户给定局域时钟脉冲周期和基准时钟脉冲周期并且把局域时钟脉冲周期与系统时钟脉冲周期同步，其中通过把局域时钟脉冲周期与分配系数相关联的方式产生同步到系统时钟脉冲周期的、至少一个用户的局域时钟脉冲周期，其中，分配系数说明基准时钟脉冲周期与局域时钟脉冲周期的比例，其特征在于，通过加或者减一个适配值或者说补偿值，对用于把局域时钟脉冲周期与系统周期同步的分配系数进行匹配。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分别利用在海参中有诱导作用的海星神经提取液和二硫苏糖醇对从成熟卵巢解剖出的刺参卵母细胞进行诱导成熟试验，结果显示，刺参的卵母细胞在海水中不会发生自发成熟，海星神经提取液以及神经提取液加滤泡悬浮液对卵母细胞没有诱导成熟的作用，而二硫苏糖醇处理可以诱导卵母细胞生发泡破裂，当DTT溶液的浓度处于10-1 mol/L到10-3 mol/L之间时，对刺参卵母细胞的促熟作用较为明显，在10-2 mol/L左右时，卵母细胞的诱导成熟率可以达到90%以上。

In respect of the nature of guarantee period, there are a few views in the theory and practice field including period of prescription, period of exclusion, and period of divesting of right. Guarantee period does not conform to the features of period of prescription and period of exclusion, guarantee period should be "the period of divesting of right clause" promised by the guarantee contracting parties.

关于保证期间的性质，理论界与实务界有种种观点，包括诉讼时效说、除斥期间说、失权期间说，保证期间不符合诉讼时效与除斥期间的特征，保证期间实质上为保证合同中当事人约定的&失权条款&上的期间。

In compliance with the objective route of historical development, and based on time and its contents, forest tourism culture in Zhejiang are divided into four periods: formation period, development period, prosperity period, and approaching modern times period; based on its representation of characteristics, it can be divided into origin and transition period, classifying and humanizing landscape and fusing forest period, enjoying and appreciating landscape and the climax of forest period, sight-seeing, exploring landscape and the science of forest period.

本论文应用历史研究的方法，从深入挖掘、系统整理史迹材料着眼，分析研究浙江森林旅游文化的渊源和流变，遵从历史发展的客观线索，按时间和内容将其分为形成期、发展期、繁荣期、走向近代的浙江森林旅游文化等四个时期；按其特征表现分为起源和过度期，归宿、"人化"山水和森林融合期，游览、欣赏山水和森林高潮期，观赏、探索山水和森林科学期等。

Layered described Cambrian - the Triassic formation, with the period of tectonic events on the sedimentary layers and structural features several important period of formation is not integrated interface, and Tajikistan low uplift tectonic evolution into paleouplift a period ( As early as the late Ordovician), paleouplift stereotypes period, the uplift of ancient period (Silurian Period to the Devonian), partial adjustment period in four phases.

分层描述寒武系—三叠系地层，结合各期构造事件研究了各层沉积构造特征及几期重要的地层不整合界面，并将塔中低凸起构造演化分为古隆起形成期、古隆起定型期、古隆起改造期、局部调整期四个阶段。

Offer for the grant of option The board is pleased to advise you that pursuant to a resolution passed at a meeting of the board held on 8th March 2007,the board has resolved to grant you the "Offer"(which is still subject to listing of committee of the stock exchange approving the granting of the listing of and permission to deal in the shares which may fall to be issued pursuant to the exercise of the options under the Pre-IPO Share Option Scheme) at a consideration of HK$1.00 an option pursuant to the Pre-IPO Share Option Scheme to subscribe for 40000 shares at an exercise price of 85% of final HK dollar price per share at which the shares are to be subscribed for /purchased and issued/ sold pursuant to the global offering of the shares in the initial public offering in or around March 2007 at any time during the six-year period commence from the expiry of twelve months from the listing dateprovided that the maximum number of shares in which you are entitled to subscribe for by exercising the option shall not exceed 25%of the total number of option shares during the period from expiry of the first anniversary of the listing date to the date immediately before the second anniversary of the listing date :and 25%of the total number of option shares during the period from expiry of the second anniversary of the listing date to the date immediately before the third anniversary of the listing date ;and 25%of the total number of option shares during the period from expiry of the third anniversary of the listing date to the date immediately before the fourth anniversary of the listing date ;and 25%of the total number of option shares during the period from expiry of the fourth anniversary of the listing date to the date immediately before the fifth anniversary of the listing date save that any of the outstanding and unexercised option at the end of each of the aforesaid vesting periods may be rolled over to the next vesting period and exercisable during the option period ,and the option shall,in any case ,lapse where you cease to be employed by the company or its subsidiaries for whatere reason or otherwise pursuant to the terms of the Pre-IPO Share Option Scheme.

登录后回答可以获得经验值奖励，并可以查看和管理所有的回答。登录|1.Offer的期权该委员会很高兴地通知您，根据是在对2007年3月8日举行的董事会会议上通过一项决议，该委员会决定授予您"要约"（依然受在联交所上市委员会批准给予上市，并准许其在交易的股票可能下跌发出依据根据首次公开招股前购股权计划之购股权获行使）在审议为港币1.00元的选择根据首次公开招股前购股权计划认购40000股为85港元，最终每股％美元的价格行使价格，股票要认购/购买并发行/出售依照全球发售的股份在最初在2007年3月左右上市期间的任何六年期开始从十二个月上市日期提供的届满时间，在你有权认购股份的最大数量行使在紧接上市日期2周年选项不得超过（在从上市日期一周年期间届满的日期一）25的选择权股份总数％：及在从该上市日期2周年期间届满之日起的25选择股份总数％，紧接上市三周年之日；25的选择权股份总数％，与从上市日期四周年期间届满的日期紧接上市日期五周年，并保存该悬而未决的和未执行的选择任何在最后上述的归属期间每年可结转到下一个归属期期间内行使的期权，而选择应在任何情况下，失误，你不再是由公司或whatere或其他原因及其附属公司雇用根据该条款前首次公开售股购股权Scheme。

Zantedeschia aethiopica constructed wetland system has high COD and N removal rate in dominant period than in vigorous period, caused by the high temperature in dominant period. Low TP removal rate in dominant period than in vigorous period caused by the following reasons, first the decay of litter in the dominant period is obvious, and the high inflow rate in the dominant period.

马蹄莲人工湿地系统在休眠期（6月和7月）和在旺盛生长期相比，由于温度高而具有高的COD，和N去除率，由于枯枝落叶的腐烂溶出以及进水负荷高而具有低的TP去除率。

Objective To study the effect of chitosan in the prevention of fibrous scar formation in the epidural space after laminectomy.

目的 观察选择性脊神经后根切除术椎板切除后硬膜外几种防瘢痕粘连物质的作用并探讨其机制。

On his way to the lift, he looked back to us or was it me his eyes pointing?

当他要进电梯时，他回头看我们（或者说是我，他的眼光？