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Restoration of beta cell mass through pharmaceutically induced endogenous beta cell mass expansion may reolutionise diabetes therapy.

通过药物诱导内源性β-细胞群膨胀来恢复β-细胞群功能可能是糖尿病的革命性疗法。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

MRI findings usually fall into 2 categories: 1 A soft tissue mass that is hypointense on T1-weighted images and hyperintense on T2-weighted images with diffuse homogeneous or slightly heterogeneous enhancement within the collection; 2 As the phlegmonous mass necroses, there may be a peripherally-enhancing fluid collection.

MRI表现通常被分为两类：1）软组织肿块，T1低信号，T2高信号，病灶内弥漫均匀性或轻度不均匀强化；2）当表现为蜂窝组织炎坏死时，表现为边缘强化的积液。

Visual carbon stars whose mid-infrared spectra are mainly dominated by photospheric continuum and show large excesses at 60um, represent a transition phase which follows an interruption of mass loss from the oxygen-rich phase and begins a new phase of the mass loss of carbon-rich material. Excesses at 60um in these stars are the emission from the residual of oxygen-rich material.

光学碳星的中红外能量主要来自光球，在60μm处存在超量发射，人们认为其演化阶段处在抛射富氧物质终止和新的富碳拱星壳层形成之间，其60μm超量发射就是富氧物质的残余效应。

In obese children, leptin concentration decreased during 4 mo of physical training and increased during a subsequent 4-mo period without physical training, fat mass was highly correlated with baseline leptin, and greater reductions in leptin during 4 mo of physical training were seen in children with higher pretraining leptin and in those whose total mass increased least.

在肥胖小孩中，4mol 周期体力运动使瘦素浓度降低，在接下来不运动的4-mo 周期中，瘦素水平提高。在那些体重增长最慢、运动前瘦素水平高的肥胖小孩中，4-mo 体力运动周期使其瘦素水平降低程度非常明显。第二篇翻译原文全文。pdf (43.99k)杂志，年，卷，期，页

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Methods and results LVH was assessed by echocardiographic LV mass index in g/m(2.7 and excess of LV mass (eLVM, in % of the observed value) relative to the amount predicted by sex, stroke work, and height, using a prognostically validated equation in 2078 participants of Cardiovascular Health Study without prevalent MI and normal systolic function.

方法和结果：通过超声心动图左心室质量指数（g/m(2.7)）和左心室剩余质量测定LVH，两者均与性别，心脏搏功，身高因素有关。

The supplement of oxygen,which was used during remineralization, was due to the mix of different water mass in vertical direction, and the gradient variation of nutrients resulted from the remineralization of organic matter in hyposurface and intermediate water mass.

6根据垂向不同水团的表观耗氧量与营养要素比值推断：水团混合为有机物矿化提供氧的补充，同时次表层和中层水团中营养盐垂直梯度变化主要是由于有机质再矿化的结果。

The ionic conductivity of polymer electrolytes equaled 3.56×10-4 S·cm-1 when the mass fraction of silicotungstate lithium was 8.5% in polymer film. The lithium ions transference number, which was measured by the method of combination of AC impedance with DC polarization, decreased with increase of the mass fraction of Li4SiW12O40 in the porous polymer film.

为了提高聚合物电解质的Li+离子迁移数，人们设法增大阴离子的体积[6~8]使其难以迁移而达到提高Li+离子迁移数的目的，很少应用含有多个Li+离子的锂盐做聚合物电解质的Li+离子载体。

The results showed that NaCl stress decreased the seedlings fresh mass and dry mass significantly. Under NaCl stress, the relative contents of Na(superscript +) and Cl(superscript -) in cells increased significantly, but the increment somewhat differed with different tissues. In root and stem, epidermal cells had the greatest increment, followed by cortex cells, and stelar cells; while in leaf, epidermal cells had the greatest increment, followed by cortex cells, spongy tissue cells, and palisade tissue cells.

结果表明：NaCl能显著降低长春花幼苗的鲜质量和干质量；对根、茎和叶片横切面X射线微区分析表明，NaCl胁迫导致长春花体内各组织细胞中Na和Cl相对含量显著增加，但在各器官、组织中分布稍有不同：与对照相比，根和茎中都是表皮细胞中增加幅度最大，中柱细胞次之，皮层细胞最低；在叶片中亦是表皮细胞增加幅度最大，依次是皮层细胞、海绵组织细胞及栅栏组织细胞。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！