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Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化；RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化；抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达，包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases， ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor， Rho-GDI alpha)，研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

Developments in the fields of immunology, biochemistry, molecular biology, cell engineering and euphenics have resulted in the determination of many tumor markers with clinical value.

近年来随着免疫学、生物化学、分子生物学、细胞工程学和遗传工程学及其相应新技术的发展，发现了许多具有一定临床价值的肿瘤标志物。

Clinical data and Methods: Laparoscopic left colectomies carried out in the Department of General Surgery, Nanfang Hospital from 2003 to 2004, on 15 left colonic cancer patients were observed and analyzed on the anatomical natures of fascias posterolateral, middle to the left colon, posterolateral to the rectum, as well as the laparoscopic location markers.

临床资料和方法：2003~2004年南方医院普通外科施行的腹腔镜左半结肠切除术15例，病例均为左半结肠癌。对腹腔镜下左半结肠后外侧、中线侧和直肠后外侧相关筋膜的解剖特点和镜下定位标志进行观察和描述。

Using ISSR molecule markers, 6 populations in 4 species of Dipelta, including D. floribunda, D. elegans, D. yunnanensis, D. wenxianensis were adopted as materials to research the genetic diversity.

本文运用ISSR分子标记技术，以采自陇南和陕南的双盾木属4个种的6个居群为材料，对双盾木属植物在分子水平的遗传多样性进行了研究。

Methods: In all the SA, blood test, serum ferritin, folacin and vitamin B12 levels and bone marrow cytology examination were done. Then gastroscopy, colonoscopy, liver and kidney function test, hemolytic laboratory indicators, serum thyroid studies and serological markers of viral hepatitis were done as needed.

对140例重度贫血患者均行血常规、铁蛋白、叶酸与维生素B12测定及骨髓细胞学检查，然后根据病情选择性行胃肠镜、肝肾功能、溶血方面实验室检查及甲状腺功能、肝炎标志物、出凝血常规检测等。

Also notice with these I went with a slightly larger 25 and 26 foot line markers to represent the later boats draft scheme.

通知还与这些我到一个稍大于25日和26日徒步路线标记代表稍后船草案计划。

RAPD and AFLP markers were used to construct the primary linkage map in a backcross population for the Pacific oyster Crassostrea gigas.

利用RAPD和AFLP标记，以一回交家系为作图群体，构建了太平洋牡蛎的遗传连锁图谱。

Methods A series of markers were used by LSAB method on 28 cases of GISTs.

应用LSAB法观察CD34等系列标记物在28例GISTs中的表达。

Genetic map of sorghum × sudan grass (314A X ZKSD) were constructed based on AFLP and RAPD markers with F2:3 population .

本文以高丹草(314A×ZKSD)的 F2：3家系作为构图群体，构建了高丹草 AFLP和 RAPD 遗传图谱。

The mapping population derived from Sorghum×Sudan grass(314A ZKSD)F2:3 family,248 indivduals were sampled,and with AFLP and RAPD markers to construct which covered 10 linkage groups with 836 cM distance.

在以高粱413A和棕壳苏丹草杂交获得的248个F2：3家系构建的作图群体中，应用AFLP和RAPD两种标记技术构建了高丹草(Sorghum×Sudan grass)的遗传连锁图谱。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。