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The six groups classified by cluster analysis of SSR markers is consistent with the available pedigree and the result of combining ability. So it is feasible to analyze the genetic diversity of maize population by SSR markers and to distinguish heterotic group by method of mixed sampling.

6个玉米群体的聚类结果与系谱来源和田间配合力测定结果基本一致,说明,用若干单株混合取样分析玉米群体SSR的遗传多样性,进而对不同玉米群体划分杂种优势类群是可行的。

The principles of several DNA Molecular markers and their application on assessment of germplasm genetic diversity,fingerprinting construction,classification of heterotic groups and heterosis prediction for maize were reviewed.The present problems and prospective of molecular markers application in germplasm resource we...

介绍几种DNA分子标记的原理,综述了DNA分子标记在玉米种质遗传多样性鉴定、绘制指纹图谱、杂种优势类群划分和杂种优势预测等方面的应用,并探讨了在玉米种质研究中存在的问题和应用前景。

Designed control points of plane form with homocentric arcs and ball markers.By the moment invariants and least-squared procedure of ellipse fit, the markers wererecognized and located automatically with high precision.

设计了平面型多重圆弧编码控制点及立体控制点,结合边缘矩的不变性和椭圆最小二乘拟合技术实现控制点的精确定位。

Then the 3D data from each stereo visionmeasuring unit can be translated into a unique frame of refrence.2. Designed control points of plane form with homocentric arcs and ball markers.By the moment invariants and least-squared procedure of ellipse fit, the markers wererecognized and located automatically with high precision.3. Studied the technology of camera self-calibration, according to the camerawith wide field of view, set up the distorted projection camera model.

通过被测物表面的控制点,将局部测量数据统一到全局坐标系中。2、设计了平面型多重圆弧编码控制点及立体控制点,结合边缘矩的不变性和椭圆最小二乘拟合技术实现控制点的精确定位。3、研究了相机的自标定技术,针对大视场相机的特点,建立了非线性相机成像模型,采用优化算法完成了相机的内、外部参数标定。

Our results demonstrate that (1) the lipogenic tumor markers CDK4 and MDM2 can be used as surrogate immunohistochemical markers for the diagnosis of malignant lipomatous tumors with high sensitivity;(2) approximately 26% of retroperitoneal/thigh UHGPS cases that were positive for PPAR-γ, CDK4, or MDM2 by immunohistochemistry showed characteristic CDK4 and MDM2 gene amplification, suggesting that a subset of UHGPS cases represent DDL despite lacking histologic evidence of lipoblasts.

我们的结果表明:(1)脂肪源性肿瘤标记CDK4和MDM2可以作为诊断恶性脂肪肿瘤的免疫标记物,且敏感性高;(2)腹膜后/大腿UHGPS免疫表达PPAR-γ, CDK4或MDM2的病例中,约有26%例显示特征性的CDK4和MDM2基因扩增,这表明部分UHGPS病例表现为DDL,虽然组织学上找不到脂母细胞。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001~0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点,远多于S群体(14)和G(21)群体,而且在53个特有位点中有9个出现频率为100%的位点,这些位点可以作为区分其它2个群体的特征性标记;S– G群体特有的位点有112个,其中有4个位点出现频率为100%,可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585,遗传距离只有0.0424,在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起,说明二者的亲缘关系很近,应属于种内群体间的关系;而W与S和G的遗传相似性系数均较小(0.7939和0.7941),相对遗传距离很大而且十分相近(0.2308和0.2305),在亲缘关系树状图上单独分出一支,也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现,W的ITS序列长度在1266-1269 bp,而S和与G的ITS序列总长度分别为1520 bp和1614 bp;从ITS1和ITS2长度来看,W分别为739-741 bp和316-317 bp,S为895 bp和414 bp,G为987 bp和416 bp;而从ITS碱基组成来看,W的GC含量在62.32-62.62%之间,而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003,S与G群体间的遗传距离是0.010,说明W群体内变异很小,而S与G群体间已出现明显的遗传分化,但还均属于种内群体间的遗传变异;而W与G和S的遗传距离分别达到0.110、0.147,两个类群差异显著,已远超出种内群体间的遗传变异。

Microsatellite markers are used to analyze the genetic background of KM outbred mice from Shanxi. The two populations were bred by mating in an outbred manner for nine generations and nineteen generations (named A9 and A19 respectively). Seven SSR markers located on seven different chromosomes were utilized in this research, D3Mit22 was selected for detailed analysis.

应用微卫星分子标记对昆明小鼠封闭群相隔10代的两个群体(分别命名为A9和A19)进行遗传背景分析,共设计位于小鼠7条染色体上的7对微卫星引物用于实验研究,选取具有多态性的一个微卫星位点D3Mit22进行详细分析。

The blood tumor markers can help to prognose if there is lymph node metastasis or not in gastric cancer patients, and combining detection of multiple markers is beneficial in improving the diagnostic accuracy.

血中肿瘤标志物可以用来预测胃癌患者是否有淋巴结转移,采用多种标志物联合检测有利于提高诊断的准确性。

Compared with the HBV immunological markers, serum HBV-DNA detection have better sensitivity, but difficult to detect the infection of Hepatitis B virus in non-replication status, and can not replace serumal HBV immunological markers.

血清乙肝病毒核酸的定量与乙肝免疫标志检测相比,有较高的敏感度,但难以检出非病毒复制状态的感染,尚不能替代血清标志物的检测,在乙肝患者诊治过程中,应联合乙肝免疫标志、HBV -DNA的检测,以助于疾病的诊断、疗效观察以及预后判断。

The watch features stainless steel case, stationary stainless steel bezel, roll stainless steel band, stainless steel link bracelet secured with a fold-over clasp, 21 jewels, screwed-down crown, scratch resistant mineral crystal, slim minute markers, discrete white Arabic numerals at five-minute intervals, and a unique dial featuring an easy-to-read day subdial surrounded by bold luminous silver-bar hour markers.

该手表的功能不锈钢表壳,固定不锈钢表圈,不锈钢卷带,不锈钢手镯的折叠式表扣,21颗宝石,螺纹表冠,防刮矿物晶体,亭亭玉立分钟标记,离散白色阿拉伯数字担保每五分钟一班,和独特的拨号具有易于阅读的一天表盘大胆的发光银杆时标包围。

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