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On the other hand,there has been so far no mapping method and software that can be used to map dominant-recessive markers obtained from F2 population of lepidopteran species.To resolve this problem,two-point and three-point maximum likelihood methods for mapping dominant-recessive markers using F2 population of this type of species were developed in this paper.At,the same time:we also gave a general method for constructing the gene linkage map of this type of species using F2 population.

另外,目前还没有任何方法和软件可以用杂交F2群体来作图鳞翅目类昆虫,如家蚕等连锁图谱,为了解决这一问题,本文发展了用F2群体作图这类昆虫连锁图谱的两点和三点极大似然法,同时还给出了大群体作图形态基因连锁图谱的一般方法。

Compared with other myeloid linage markers, MPO was the most sensitive and specific markers in the diagnosis of acute myeloid leukemia.

结论流式细胞仪检测MPO的标准推荐方法:应用FACS破膜剂15 min,标本必须当天完成检测,尽量采用PE标记的MPO抗体。

The results revealed that nine of the fourteen Y-specific markers cannot be used for investigating the swamp buffalo Y chromosome genetic diversity because of the reasons specified below: three microsatellites markers (INRA008, UMN0103 and UMN0504) were single allele and monomorphism, three (UMN1113, UMN0304 and BC1.2) indicated three alleles respectively but mornomorphism, and the other three (UMN0920, UMN0307 and UMN3008) showed irregular ladder-like bands. Only the remaining five microsatellites (INRA124, INRA189, BM861, PBR1F1 and UMN2001) indicated polymorphisms, and thus can be applied to study the swamp buffalo Y chromosome genetic diversity.

结果表明,3个标记(INRA008,UMN0103和UMN0504)只有1个等位基因,表现为单态;3个标记(UMN1113,UMN0304和BC1.2)均为3个等位基因,但呈单态;3个标记(UMN0920,UMN0307和UMN3008)呈现无规律的梯状条带,所以这9个标记都不适用于水牛的Y染色体遗传多样性研究;只有5个标记(INRA124,INRA189,BM861,PBR1F1和UMN2001)具有多态性,表明适用于水牛的Y染色体遗传多样性研究。

The research advances in genetic relationships of peach germplasm, including the morphologic, cytology, palynology, biochemistry and DNA molecular markers, were reviewed in this paper. The actuality, prospect and urgent problems of peach germplasm resources were also discussed. To further study the genetic relationships of wildness, sib wildness and cultivar groups of peach, we may resort on genetic markers and DNA sequence analysis in addition to morphological characters.

本文从形态学、细胞学、孢粉学、生物化学及DNA分子标记等几个方面综述了桃种质资源亲缘关系的研究进展,探讨了桃种质资源亲缘关系的研究现状、前景及尚需解决的问题,并就进一步开展桃种质资源亲缘关系的研究进行了分析,提出桃种质野生种、近缘野生种厦不同栽培品种群间的分子系统学关系是今后研究的重点。

Methods Microsatellite markers of mice and rat were adopted to develop markers and methods suitable for genetic monitoring for SPF golden hamster population and its parent conventional population from Chengdu Institute of Biological Products by fluorescence-based semi-automated genotyping method. Genetic diversity and differences of the 2 golden hamster populations were analyzed based on the result of microsatellite alleles.

应用小鼠和大鼠的微卫星标记筛选适于金黄地鼠遗传检测的微卫星标记,并结合微卫星荧光标记-半自动基因分型技术,对成都生物制品研究所的SPF级金黄地鼠及其来源的普通级金黄地鼠进行遗传检测,计算其群体遗传学参数。

These markers have had a revolutionary impact on the study of genetic structure and variation of pearl oyster, With genetic markers, they have allowed rapid progress in better strains selection and effective protection.

运用各种遗传标记对海洋珍珠贝的遗传结构及多样性进行研究,有利于提出有效的保护和复壮方案,加速良种的选育。

The knitting went pretty smoothly for this one, except on the third day. I was watching CSI, and somehow added one stitch to the right of the leaf lace panel. All the increases are supposed to be along the raglan sleeve lines where stitch markers were placed. There are also two stitch markers for the leaf lace panel to mark the beginning and the end. I was really into what was on TV and didn't look to see which stitch marker I was at, I did a M1 before the lace panel marker...

编织过程其实很顺利,只有在第三天时因为太专心看CSI,在叶子蕾丝右侧记号圈前加了一针,它是应该延著拉克兰袖线记号圈处加针,蕾丝两侧也放记号圈,但只是用来标示蕾丝,啊我顾看电视碰到记号圈也没看清楚就给它加针。。。

Collagenase and 25 m filtration web, and characterized in terms of the expression of different phenotypic markers of osteoclasts and their ability to resorb bone matrix. The phenotypic markers of DCs include the expression of tartrate-resistant acid phosphatase, acid phosphatase, resorption pit assay, reaction to calcitonin and identification of F-actin.

于37oC,5%CO。培养箱中培养1-2小时后,弃培养液:lxPBS清洗数次;0.25%胰酶-EOTA 37℃消化5分钟,加入含川%FBS的a-MEM,经小25tim过滤网过滤;IX PBS清洗末过滤细胞,即得到纯化的破骨样细胞,并进行细胞形态学和分于生物学鉴定。

Objective To study the change of bone formation markers and bone resorption markers in women of different ages.

目的 探讨骨吸收与形成指标在不同年龄组妇女中的变化趋势。

The genetic relationship of 27 sapodilla cultivars and 4 mamey sapoae cultivars were analyzed by using fluorescent-labeled AFLP markers. A total of 1131 AFLP markers between 75 to 500 base pairs were generated with 9 primer combinations, of which 1096 (96.9%) were polymorphic.

利用荧光AFLP分子标记技术对27个人心果品种及4个蔓妹人心果品种资源的亲缘关系进行研究。9对引物共扩增得到1131条带(75~500 bp),其中多态性带1096条(96.90%)。28个品种具特征性条带,特征带大小为350~500 bp。

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