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A F2 population derived from a cross between apetalous line' APT02 'and normal petalled cultivar 'ZS NO.4' was used for molecular markers and mapping of the gene controlling petal-loss trait in Brassica napus.20 pairs of AFLP primers and 170 pairs of SRAP primers were selected between two parents.

在无花瓣品系APT02和正常有花瓣品种中双4号构建的的F2分离群体中，运用AFLP和SRAP两种标记技术对甘蓝型油菜花瓣缺失基因进行分子标记和图谱定位。

The genetic diversity of Leizhou Peninsula five Avicennia marina mangrove populations used of ISSR markers analysis.

利用ISSR标记技术研究了雷州半岛5个红树林白骨壤种群遗传多样性和遗传分化。

Two RAPD molecular markers located on different arms of 2Ai-2 chromosome from Thinopyrum intermedium were selected and the generations of the hybrid generation derived from Zhong 4 Awnless and wheat were analyzed. Two chromosome translocation lines were screened for wheat breeding program to control BYDV.

本研究采用RAPD方法，对具2Ai-2端体和完整染色体异附加或异代换系进行分析，从随机引物中，选出两个分别位于2Ai-2染色体两臂上的分子标记，然后用这两个标记对中间偃麦草和普通小麦的杂交后代进行筛选，选出小麦抗黄矮病易位系新材料。

Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager 2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating populations.

利用SSR分子标记结合&拟测交&策略，以荷包红鲤抗寒品系和柏氏鲤远缘杂交所产生F1代为亲本及其F2代为作图群体，应用Windows Map Manager 2.0软件的标记回归法进行乳酸脱氢酶(Lactate dehydrogenase， LDH)活性数量性状基因座单标记定位分析。

Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating popula-tions.

利用SSR分子标记结合&拟测交&策略，以荷包红鲤抗寒品系和柏氏鲤远缘杂交所产生F1代为亲本及其F2代为作图群体，应用Windows Map Manager 2.0软件的标记回归法进行乳酸脱氢酶(Lactate dehydrogenase， LDH)活性数量性状基因座单标记定位分析。

Genetic variation was evaluated using phenotypic traits and RAPD markers in 8 natural populations of Betula luminifera from Fujian Province, China. The relationship between genetic variation and environmental factors was also examined.

采用表型性状测定结合RAPD标记，研究福建境内8个光皮桦野生种群的遗传变异，探讨遗传变异与环境因子间的相关性。

The set of SSR loci would potentially be useful molecular markers for the germplasm identification, genetic diversity analysis, and gene mapping of Bupleurum.

从文库资料中挖掘的具有潜在标记价值的SSR位点将为柴胡种质鉴定，遗传多样性分析及基因定位提供有效分子标记。

Methods: 12 fresh frozen adult leg-foot specimens were mounted in a coordinated system, a Steinmann pin was perforated across the calcaneus, cuboidcum, talus, and naviculare as markers. The displacements of the marked points in the coordinated system were recorded. Through matrix translation and Euler equation, the rotational angle of the calcaneus, cuboideum, talus, naviculare were calculated, which represented the motion of the calcaneocuboid, talonavicular and ankle joint in different condition respectively, while statistical analysis were also taken between different conditions.

取新鲜足标本12例，通过加载使足产生某种形式的运动，用三维数字化坐标仪测量距下关节组成骨在某种运动状态下的相对三维坐标位移，通过矩阵转换和求解非线性函数方程计算其三维旋转角度，确定距下关节的运动范围、规律和在足整体运动中的作用。

Morphological change of cells was observed by microscope,α-MHC and β-MHC, used as the markers of cardiogenesis expression of genes during the differentiation, were determined by RT-PCR.

结果表明，P19细胞经诱导后形态发生明显变化；α-MHC在诱导分化6d时开始表达，β-MHC在诱导分化8d时开始表达，表明心肌分化成功。

Random amplified polymorphic DNA markers were used to measure genetic diversity and genetic structure of 3 natural populations of Carya dabieshanensis 238 loci were identified by 20 RAPD primers, out of which 162 loci were polymorphic and accounted for 68.1% of the total genetic diversity at species level.

利用RAPD分子标记技术检测了大别山山核桃3个天然居群的遗传多样性和遗传结构。20条10bp随机引物共检测到238个扩增位点，其中多态性位点162个，多态位点百分率为68.1%。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。