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Fifty-three primer combinations were found to amplify nuclear DNA of Chinese cabbage, properly. They amplified the fragments up to 4 381 with an average of the 83. Among them, 9 markers derived from 9 primer combinations were linked to the clubroot resistance gene.

筛选出的适宜53对引物组合共扩增出4381条带,占总扩增片断的33.50%,平均每对扩增出83条,其中9个组合扩增出与抗根肿病基因连锁标记。

The result indicated that 237 combinations amplified a total of 13 079 AFLP fragments with an average of 55 fragments, the average of 1.30 markers per 1 000 AFLP fragments were found to be linked to clubroot resistant gene; the amplified polymorphic fragments were 3 167 between the two parents, account for 24 percent of polymorphic frequency; the number of amplified fragments and the number of polymorphic fragments was in positive linear correlation; GC content of AFLP primer combinations significantly affects the number of amplified fragments and the number of polymorphic fragments, indicating negative linear correlation between them.

结果表明:平均每个组合检测到55条带,共扩增13079条带,平均每1000条带检测到1.30个根肿病抗性基因连锁标记;多态性带数为3167条,双亲间多态性频率约为0.24;扩增带数与多态性带数呈线性正相关,引物组合中的不同GC含量与扩增带数和多态性带数存在显著性差异,并表现出线性负相关。

objective: to investigate the variation of coagulation and fibrinolytic molecular markers in tumor patients.

目的:探讨肿瘤患者凝血及纤溶分子标志物的变化情况。

Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.

方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平,并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素,全自动血液凝固仪检测凝血酶原活动度,同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。

Secondly, quantifying markers: Because genes polymorphism or the codominant molecular marker electropherogram in a gene locus present 3 states, we can quantify them with -1,0 and 1 respectively.

将标记的在电泳时表现的三种状态:隐性纯合、杂合及显性纯合分别量化为"-1"、"0"和"1"。

A statistical method was described for interval mapping of quantitative trait loci using codominant flanking molecular markers such as RFLPs.

本文系统地描述了应用两个相邻共显性分子标记进行数量性状基因座位区间作图的方法。

Both of them, to be codominant markers, can identify false hybrids not only from selfed seeds of male and female but also from foreign pollen and mechnical contamination.

两引物均能在父母本中扩出特异带,为共显性标一记,不仅能够鉴别杂交种中混杂的母本自交的种子和父本的种子,还可鉴别部分由于外来花粉或机械混杂等造成的假杂种。

It is suggested that it is better to indicate the variability within breeds by average heterozygosity based on microsatellite polymorphisms, which showed high polymorphisms and codominant inheritance of the markers, where as differences between populations and even specific characteristics with in a population are reffected clearly and directly by RAPD.

由于微卫星标记多态性程度高且等显性遗传,因而通过它计算杂合度可以较好地反映群体内的变异;RAPD则在反映群体间的区别甚至某一群体的特征时可提供明确而直接的信息。

Based on analysis of Wx of D4063-1, indica and japonica and according to the special sites of the three species, primers as markers-AUT4063-I were designed to distinguish D4063-1 from other rice. Combining with primer pair F5, dominant and codominant ways were established for discriminating them, and rapid and correct identification of D4063-1 from other rice could be done.

本研究还根据D4063-1和籼稻、粳稻的序列差异及D4063-1在该片段上的特征序列位点设计了用于识别D4063-1的寡核苷酸片段,并作为PCR反应的引物命名为AUT4063-1,将该引物与作者设计的扩增普通籼稻、粳稻Wx基因的引物F5配合使用,建立了识别D4063-1的显性和共显性两种检测方式的分子标记,为快速、准确鉴别低直链淀粉含量突变体D4063-1创造了条件。

Since the SSR DNA markers have codominant inheritance, it makes study genetic recombination of the RIL individuals become possible.

由於本研究所采用之SSR分子标志具有共显性,故可以SSR分子标志探讨水稻各RIL之遗传重组情形。

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