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Common buckwheat ( F. esculentum ) is an allogamy crop, which is heterogony. There is heterogeneity in the species. To reveal the genetic diversity within cultivar population, In order to explore the sampling strategy that can reveal genetic diversity of buckwheat resources on the molecular level. PCR with intron-splice junction primers and long random primers was employed to reveal the genetic diversity within nine common buckwheat varieties and one tartary buckwheat cultivar population among different individuals. We established the sampling strategy in its genetic diversity study. Then we used the established sampling strategy, and the same PCR markers technique,to analysis the genetic diversity of 86 common buckwheat resources, the main results are as follows

甜荞是花柱异长自交不亲和的异花授粉作物,品种内存在一定的异质性,为了准确揭示甜荞遗传资源的遗传多样性,本研究采用因子分析法对86份甜荞遗传资源农艺性状数据进行分析;采用长随机引物和内含子切接点引物的PCR分子标记技术,对9个甜荞品种和1个苦荞品种不同个体间的遗传多样性进行了分析,建立了甜荞资源遗传多样性研究的取样策略;并利用建立的取样策略,采用PCR标记技术,对这86份甜荞资源的遗传多样性进行了分析,取得如下主要结果

In RPE cell culture, Sox2 promoted the expression of retinal ganglion and amacrine markers, and suppressed the expression of genes associated with RPE properties.

在RPE细胞培养中,Sox2促进了视网膜神经节细胞和无长突细胞标记物的表达,并抑制RPE功能相关的基因的表达。

GDCA, GCDCA, GCA, and GUDCA might be used as potential markers to indicate degree of amanita verna poisoning and prognosis of patients.

GDCA、GCDCA、GCA及GUDCA可以指示白毒伞中毒程度及预后。

Flow cytometry and immunofluorescence were applied to detect the expression of surface markers of amniotic fluid stem cells.

流式细胞仪及免疫荧光法检测羊水干细胞表面标志的表达。

In this study, the author used GISH technology to analyze the material that was resistant to take-all in test, and got three chromosome arm addition lines that carried the resistance genes derived from the amphiploid of Triticum-Haynaldia. The above arm addition lines and their resistant and sensitive parents were analyzed by RAPD method and two specific molecular markers that were tightly linked to resistant gene were obtained.

本研究先用基因组原位杂交对经鉴定抗全蚀病的材料进行分析,检测出三个抗性源于簇毛麦的端体异附加系材料,再利用RAPD方法,对这三个材料和抗感病亲本进行分析,得到了两个与全蚀病抗性基因紧密相关的特异性RAPD标记。

Sequences of ITS in nrDNA is one of the widely used markers in angiosperm systematics.

核糖体rDNA ITS是被子植物系统发育研究中应用最广泛的分子标记之一。

The typical pathological characteristics of SPTs may result from gradual degenerative changes induced anoxemia in some SPT's areas. The heterogeneity of SPTs on different antibody markers showed that the SPTs may be originated from pancreatic embryonic stem cells, and result from immature differentiation of the pluripotential stem cells during pancreatic genesis.

SPT典型病理特征是由于肿瘤组织不同区域相对缺血、缺氧所造成的退行性变的结果,SPT表达多种组织来源的抗原,反映其可能来源于胰腺干细胞及与其发育密切相关的胚胎神经嵴的神经前体细胞,且在发育过程中发生分化不成熟的结果。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞,运用活细胞计数和碘化丙吮检测其增殖能力;采用流式细胞术检测其细胞表面标志的表达;用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化,并用Von Kossa染色进行鉴定;用地塞米松与胰岛素诱导其向脂肪细胞分化,并以油红O染色进行鉴定。

This paper first introduces the theory and method of vehicle automated driving based on magnetic markers,analyzes the results of vehicle automated driving based on the apery control and points out the shortcoming of it.

介绍了基于磁道钉导航的车辆自动驾驶的原理和实现方法,分析用此方法建立仿人控制模型来实际控制车辆自动驾驶的结果,指出仿人控制模型的不足之处。

Sport watch, Swiss-Made automatic movement displaying 6-hour, 30-minute and 60-second subdial, Main dial polished silver-tone hour/minute hands with luminous accents and polished chronograph-seconds sweep hand, Subdials displayed by silver-tone hands, Main dial applied and polished silver-tone luminescent baton markers, White Arabic numbers/indices mark subdials, Red/white numbers and white indices mark outer minute track, Analog date display at 3, Polished silver-tone stainless steel round bezel and case with raised design at case sides, Polished screw-in crown and steel pushers, Stainless steel screw-in 'skeleton' caseback, Sapphire crystal, 200 meters/660 feet water resistant.

运动手表,瑞士制造的自动运动显示6小时,30分钟和60秒的表盘,抛光银主拨号音小时/分钟双手发光口音和抛光计时,秒扫手,Subdials显示的银色色调手,主要应用于拨号和抛光银双色荧光指挥棒标记,白色阿拉伯数字/指数马克subdials,红/白,白指数的数字标记外分钟跟踪,模拟3日期显示,抛光银双色不锈钢圆形边框并设立案件提出了设计方面的情况,抛光螺钉在'在冠和钢铁顶推船,不锈钢螺钉骨架'表壳,蓝宝石水晶,200 meters/660英尺防水。

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It is suggested that TDS can be used as a new method for the analysis of pedogenesis and climatic change.

总之,热处理过程中磁化率的变化能非常灵敏地反映磁性矿物的转化情况,而且,这种转化往往与样品所经历的土壤化作用强度密切相关,因此,TDS可做为分析成土作用强度和古气候变化的一种新方法。

Therefore, in this chapter, I will research into the conflict and contradiction of the paternity. Also, in this novel, the father's behavior of "He who loves well chastises well." is quite suspicious, one of the reasons is that the father thought his son belonged to him. And then, this thesis quoted Lacan's theory to touch upon that someone who has accepted the concept of patriarchal society, he will be influenced for his whole life. That also reveals that human being has no choice sometimes in this society.

因此本章会先就父子关系的冲突与矛盾做深入的探讨,小说中的父亲似有对儿子爱之深责之切的行为,其原因可能在於父亲将儿子视为所有物所致;其次,论文将引用拉冈相关理论,讨论在父权社会中,不论身分为何,只要是已受父权社会观念根深蒂固影响的人物,终生都将受其所苦,此也揭示出人生存於社会上的无奈。

But these days, because of everybody's attention,"Super Marie " track begins suddenly to become eccentric, see a chess do not go out only action, be in it seems that sedulous and evasive what.

但这几天,因为大家的关注,"超级玛丽"的行踪突然开始变得古怪,只看棋不出招,似乎在刻意回避着什么。