查询词典 marker

Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.

利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明，Am6-4中的抗白粉病和抗条锈病基因均为单显性基因；以124对D基因组SSR引物进行标记分析，引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带，标记与抗白粉病基因间的遗传距离为20.42cM，并将抗白粉病基因定位于6D染色体；引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带，标记与抗条锈病基因间的遗传距离为8.0cM，并将抗条锈病基因定位于1D染色体。

The system gets two image of one object with two CCD, thus obtains depth from two CCD"s parallax. After investigating the operation spot and discussing with surgeons we use LED as markers, and get the marker"s 3-D position with a non-contact optics orientation method. After calibrating the CCD, processing the image, the system can figure out marker"s areal coordinates, get marker"s 3-D coordinate in dummy world coordinate system, thereby obtain instruments 3-D position and orientation. After the coordinate transformation and a series of orientation calculation, operation navigation comes true.

运用两个摄像机对同一目标从不同位置成像，进而从视差中恢复距离；通过对手术现场的仔细了解与手术医师的反复商榷，使用LED作为标志点，利用非接触式光学定位方法提取空间点位置信息：通过对摄像机的标定、图像处理及目标点重心的计算，求出空间点在虚拟世界坐标系下的三维坐标，从而确定了手术器械等的空间位姿，通过空间坐标转换及一系列定位计算，完成了手术导航实验系统。

Concept of culture Shenzhen is a city of speed and we can make a response with the marker's external form and composite streamline system; Shenzhen is a city of diversity and the marker's performance at different visual angles and function composition is its external manifestation; Shenzhen is a city of innovation, marker is the external form and composite function carrier of its urban wonders, a brand-new experience in behavior is triggered when a super strong attraction is generated.

文化 深圳是个速度的城市，我们通过标志物外在形式及复合流线体系予以回应；深圳是个多样的城市，标志物不同视角的演绎与功能复合是其外在体现；深圳是个创新的城市，标志物以其城市奇观外在形式与复合功能载体，产生超强行为吸引力的同时触发全新行为体验。

An RAPD marker was tightly linked to Fulvia fulva resistant gene Cf6 in tomato, with a genetic distance of 8.7 cm, The RAPD marker was converted into SCAR marker.

经F2单株分析，在后代群体中扩增出了两条长约500 bp的特异片段，该特异标记与叶霉病抗性基因Cf6紧密连锁，遗传距离为8.7 cm。

The method is simple, has safe operation, creates an approach utilizing a plant gene to safely select the marker gene for the tomato genetic transformation, can replace the disadvantages caused by the widely used heterogenous genes such as antibiotic genes, weedicide resistant genes, xyl A, pmi, GUS and so on, taken as marker genes, obtains a transgenic tomato with food safety, biological safety and ecological safety, relieves the public from anxiety of marker gene safety, and inevitably facilitates the commercialized application of the transgenic tomato.

该方法简单，操作安全，开创了利用植物基因作为番茄转化安全筛选标记基因的途径，能够代替目前广泛使用的抗生素基因、抗除草剂基因以及xylA、pmi、GUS等异源基因作为标记基因存在的弊端，获得具有食品安全性、生物安全性和生态安全性的转基因番茄，解除公众对标记基因安全性的顾虑，必将促进转基因番茄的商业化应用。

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列，结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个，而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低；CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记，同时它也能够用于雀形目鸟类的物种鉴定，但在物种鉴别方面不如细胞色素b基因稳定、准确。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Domestic and overseas entomologist has showed that different molecular marker methods for molecular identification of Trichogramma species. However, each of molecular marker methods previously has own deficiency, and molecular marker genes used in Trichogramma species identification almost is ribosomal DNA, study about mitochomadrial DNA in Trichogramma spp. is infrequent.

近年来，随着分子生物技术的发展，国内外研究者曾用不同分子标记方法来进行赤眼蜂蜂种的分子鉴别，然而每种分子标记都有自己的不足，且赤眼蜂分子鉴定中用于序列分析的标记基因基本上为核糖体核糖核酸基因，而有关线粒体DNA的研究却鲜有报道。

Resistant accessions, genetic basis, biochemical research and molecular marker are summarized in this article. We also discussed how to apply allelism test which using hybridize and molecular marker in different resistance materials in order to make a good use of resistant resources and provide useful information in the further research of the multiresistant mungbean breeding.

本文就绿豆抗豆象基因资源的发掘与利用及抗豆象特性的遗传机理与分离规律进行了总结与分析，对抗豆象的生化基础研究和分子标记进行了综述，探讨了抗豆象育种的重要性及尚需解决的问题，提出进行不同抗源的基因等位性鉴定、高效地利用抗源、培育多价抗豆象绿豆品种的重要性。

According to our present broadcasting situation,the thesis proposes using new technology to update and reform the present HFC. It also suggests broadcasting using self-advantage and arranging EPON in order to win the supermarket.

论文从我国广电的现状出发，结合实际，积极倡导采用新技术更新、改造我国现有的HFC网络；倡导广电现阶段利用自身优势，部署EPON网络来赢取市场。

A new general kinetic equation was derived in which the effect of the removal of condensation water was taken into account completely, and was applied to the polyesterification kinetic study of AA/HPHP and AA/NPG. It was found that the reaction order of self-catalyzed polyesterification was not constant. For these polyesterifications, the reaction order was 2.5 at a low temperature, while 3.0 at a high temperature.

考虑缩合水的排除对反应体系的影响，用体积浓度单位推导出一个新的聚酯反应总包动力学方程，并应用到AA/HPHP和AA/NPG两个聚酯化反应的动力学研究中，研究发现，自催化聚酯反应的反应级数并不是固定值，对于AA/HPHP和AA/NPG两个聚酯化反应而言，低温时反应级数为2.5，高温时反应级数为3.0。