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mannosan相关的网络例句

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与 mannosan 相关的网络例句 [注:此内容来源于网络,仅供参考]

By the analysis of SDS-PAGE electrophoresis, CXJZ95-198 secrets exocellular proteins bands in media of glucose, mannose ,xylan, mannosan and pectin respective were 32 bands, 33 bands,35 bands, 36 bands and 21 bands respectively .

在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中的发酵液在SDS-PAGE电泳中分别分泌出32条,33条,35条,36条和21条蛋白带;多数可比较的蛋白质电泳谱带的颜色深浅呈魔芋粉>甘露糖>木聚糖>葡萄糖>果胶的趋势。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点,发酵液经离心,丙酮沉淀,Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品,比活力较原酶液提高了9.8倍,回收率达到了32.98%,用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

本论文对CXJZ95-198菌株在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中胞外酶蛋白质总量、还原糖及果胶酶活性的影响进行了研究;对果胶酶、木聚糖酶及β-甘露聚糖酶在甘露糖培养基中的酶活进行了比较;对CXJZ95-198菌株在不同碳源培养基中的发酵液在SDS-PAGE电泳中分泌出的蛋白带作了比较;对CXJZ95-198菌株所产生的胞外酶系中果胶酶进行了分离纯化。

A pectinase from CXJZ95-198 was purified. Results have made know:(1) The changes of protein content secreted by CXJZ95-198 in the five media glucose mannose xylan mannosan and pectin were 1.28mg/ml, 1.17mg/ml, 0.94mg/ml, 0.75mg/ml and 0.73mg/ml.The pectinase activity was maximum in the mannose (79.39U/ml) with the order of glucose(73.33 U/ml) mannosan(24.25 U/ml) xylan(17.45 U/ml) and pectin(15.98 U/ml).

在本研究条件下,得到如下结论:(1) CXJZ95-198菌株在以葡萄糖作为碳源的培养基中蛋白质含量变化最大,达到了1.28mg/ml,其次依次为甘露糖、木聚糖、魔芋粉和果胶培养基,分别为1.17 mg/ml,0.94 mg/ml,0.75 mg/ml,0.73 mg/ml;在这五种培养基中,以甘露糖培养基中果胶酶活性最高,达到了79.39 U/ml,其次依次为葡萄糖培养基,魔芋粉培养基,木聚糖培养基,最后为果胶培养基,分别为73.33 U/ml,24.25 U/ml,17.45 U/ml,15.98 U/ml。

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